Bilobalide B nano lipidosome and preparation method thereof
A nano-liposome and ginkgolide technology, which is applied in the directions of liposome delivery, pharmaceutical formulations, and non-active ingredients medical preparations, can solve the problems of short half-life, poor water solubility, and large toxic and side effects, and achieve uniform quality. , the effect of small particle size and convenient preparation process
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Embodiment 1
[0040] Dissolve 10mg of ginkgolide B, 180mg of egg yolk lecithin, 50mg of cholesterol, 5mg of vitamin E, 65mg of cultured phosphatidylethanolamine, and 35mg of phospholipid polyethylene glycol maleimide in 20mL of absolute ethanol, and ultrasonicate for 10 minutes to form a uniform lipid The lipid solution was transferred to a 250mL round bottom flask, and the above lipid solution was distilled under reduced pressure at 35°C until no ethanol remained, and a uniform lipid film was formed on the inner wall of the round bottom flask. The prepared lipid film was hydrated with 5 mL of phosphate buffered saline (PH=6.0) at 30° C. for 1 hour, and stirred and dispersed evenly under nitrogen protection. The liposome solution was extruded multiple times with a liposome extruder, and passed through polycarbonate membranes with pore sizes of 0.8 μm, 0.4 μm, 0.2 μm, and 0.1 μm in sequence. Under the protection of nitrogen, add disodium hydrogen phosphate solution to increase the pH to 7.2,...
Embodiment 2
[0042] Dissolve 30mg ginkgolide B, 520mg dipalmitoyl lecithin, 140mg cholesterol, 16mg vitamin E, 195mg cultured phosphatidylethanolamine, 100mg phospholipid polyethylene glycol maleimide in 30mL chloroform:methanol=2:1 Mix the solution, ultrasonicate for 20 minutes to form a uniform lipid solution, transfer it to a 500mL round-bottomed flask, and under the protection of nitrogen, distill the above-mentioned lipid solution at 30°C under reduced pressure until no solvent remains, and a uniform lipid solution is formed on the inner wall of the round-bottomed flask. lipid membrane. The prepared lipid film was hydrated with 15 mL of phosphate buffered saline (PH=6.5) at 45° C. for 1 hour, and stirred and dispersed evenly under nitrogen protection. The liposome solution was extruded multiple times with a liposome extruder, and passed through polycarbonate membranes with pore sizes of 0.8 μm, 0.4 μm, 0.2 μm, and 0.1 μm in sequence. Under the protection of nitrogen, add disodium hyd...
Embodiment 3
[0044] Dissolve 50mg of ginkgolide B, 1000mg of soybean lecithin, 230mg of cholesterol, 25mg of vitamin E, 330mg of cultured phosphatidylethanolamine, 170mg of phospholipid polyethylene glycol maleimide in 50mL of dichloromethane, and ultrasonically form a uniform lipid solution , transferred to a 1000mL round bottom flask, and under the protection of nitrogen, the above lipid solution was distilled under reduced pressure at 30°C until no dichloromethane remained, and a uniform lipid film was formed on the inner wall of the round bottom flask. The prepared lipid film was hydrated with 25 mL of phosphate buffered saline (PH=6.8) at 30° C. for 1 hour, and stirred and dispersed evenly under nitrogen protection. The liposome solution was extruded multiple times with a liposome extruder, and passed through polycarbonate membranes with pore sizes of 0.8 μm, 0.4 μm, 0.2 μm, and 0.1 μm in sequence. Under the protection of nitrogen, add disodium hydrogen phosphate solution to increase ...
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