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Determination method of cerium ion based on nano-gold simulated peroxidase

A technology of peroxidase and nano-gold, which is applied in the field of analytical chemistry and nanometers, can solve the problems of being easily interfered by environmental factors and poor sensitivity, and achieves the effects of simple and fast preparation process, good selectivity and high detection sensitivity

Active Publication Date: 2021-06-25
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has poor sensitivity and is easily disturbed by environmental factors, which limits their application to a certain extent.

Method used

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  • Determination method of cerium ion based on nano-gold simulated peroxidase
  • Determination method of cerium ion based on nano-gold simulated peroxidase
  • Determination method of cerium ion based on nano-gold simulated peroxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 500 μL of 0.1 g / L chloroauric acid aqueous solution was diluted with 39.5 ml of water, and 0.8 ml of 0.1 g / L sodium borohydride aqueous solution was added under vigorous stirring (the addition time was controlled within 5 minutes), and the color of the reaction solution was From light yellow to wine red, continue to stir rapidly in the dark for 1 hour. The obtained nano-gold solution is stored at 4°C, and the nano-gold can be kept for at least two months and is relatively stable. All glassware used in the above process was soaked in aqua regia, washed thoroughly with double distilled water, and dried.

Embodiment 2

[0039] 0.2 mL of Ce at a concentration of 1 μmoL / L 3+ Add 0.05 mL of the nano-gold solution prepared in Example 1 to the solution, mix well, and then add 0.65 mL of acetate buffer solution (pH=5, 100 mmoL / L) and 0.05 mL of the solution at a concentration of 5 mol / L to the mixed solution. L of hydrogen peroxide and 0.05 mL of 3,3',5,5'-tetramethylbenzidine hydrochloride with a concentration of 8 mmoL / L were mixed and incubated at 25°C for 10 minutes. Set without Ce 3+ Blank control group. Depend on figure 1 It can be seen that adding Ce 3+ After that, the color of the catalytic reaction system solution was significantly darkened (in the figure A: nano-gold; B: nano-gold + Ce 3+ ).

Embodiment 3

[0041] 0.2 mL of Ce at a concentration of 1 μmoL / L 3+ Add 0.05 mL of the nano-gold solution prepared in Example 1 to the solution, mix well, and then add 0.65 mL of acetate buffer solution (pH=5, 100 mmoL / L) and 0.05 mL of the solution at a concentration of 5 mol / L to the mixed solution. L of hydrogen peroxide and 0.05 mL of 3,3',5,5'-tetramethylbenzidine hydrochloride with a concentration of 8 mmoL / L were mixed and incubated at 25°C for 10 minutes. Set without Ce 3+ Blank control group. Depend on figure 2 It can be seen that adding Ce 3+ After that, the absorbance value of the catalytic reaction solution at 652 nm (A 652 ) significantly increased (A in the figure: Au nanoparticles; B: Au nanoparticles + Ce 3+ ).

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Abstract

The invention discloses a method for measuring cerium ions based on nano gold simulated peroxidase. Utilizing the change of peroxidase activity simulated by the interaction between gold nanoparticles and cerium ions, gold nanoparticles catalyze hydrogen peroxide to oxidize 3,3',5,5'-tetramethylbenzidine hydrochloride for color development, thereby Shows changes in solution color and UV absorption spectral features. The linear range of absorbance value determination is 10~160 nmol / L, and the detection limit is 2.2 nmol / L. This method can be potentially applied to the determination of cerium ion content in environmental systems.

Description

technical field [0001] The invention relates to a rapid content determination method for cerium ions using nano gold as a simulated peroxidase, and belongs to the fields of analytical chemistry and nanotechnology. Background technique [0002] As the most abundant lanthanide element, cerium (Ce) has been widely used in industry, agriculture, forestry and animal husbandry in the past century. In vivo, cerium affects the function of the blood, immune system, skin, bone tissue, liver, heart and central nervous system. Cerium can be inhaled into the lungs with the air, causing pulmonary embolism and liver dysfunction. Therefore, it is of great practical significance to establish a rapid, sensitive and selective method for the determination of cerium. At present, techniques such as X-ray fluorescence, inductively coupled plasma-atomic emission spectrometry, inductively coupled plasma-mass spectrometry, neutron activation analysis, and ion-selective electrodes have been successf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N21/78
CPCG01N21/31G01N21/78
Inventor 邓豪华陈伟何少斌罗邦悦彭花萍
Owner FUJIAN MEDICAL UNIV
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