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Preparation method of hydrogel film with high adhesion to skin and function of promoting wound healing

A technology for wound healing and hydrogel film, which can be used in skin diseases, medical preparations with non-active ingredients, non-active ingredients of polymer compounds, etc. It can solve the problems of poor skin fit and poor film-forming performance, and achieve excellent Adhesion, excellent biocompatibility, effect on reducing trauma scars

Active Publication Date: 2019-04-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, these hydrogels have poor film-forming properties and can only be used in the form of smearing, or they can form films but have poor adhesion to the skin, which shows certain defects in actual clinical applications. Therefore, there is an urgent need in this field A hydrogel film that adheres to the skin and promotes wound healing

Method used

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  • Preparation method of hydrogel film with high adhesion to skin and function of promoting wound healing
  • Preparation method of hydrogel film with high adhesion to skin and function of promoting wound healing
  • Preparation method of hydrogel film with high adhesion to skin and function of promoting wound healing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Synthesis of mono-6-deoxy-p-toluenesulfonyl chloride cyclodextrin (β-CD-6-OTs):

[0030] Dissolve β-cyclodextrin (β-CD) (17.0g, 15.0mmol) in 200mL of 1% NaOH solution, and add 15mL of p-toluenesulfonyl chloride (4.0g, 22.5mmol) dropwise at 0°C ) in acetonitrile solution, stirred and reacted at 25°C for 2h, then filtered, adjusted to pH 2 with hydrochloric acid, kept at 4°C overnight, a large amount of precipitates precipitated, filtered, and the solid was recrystallized 3 times to obtain a white solid, dried in vacuo at 40°C for 5h, to obtain Mono 6-p-toluenesulfonyl cyclodextrin, spare.

[0031] (2) Synthesis of mono-6-deoxy-dopamine cyclodextrin (β-CD-6-DA):

[0032] Take 1.00g (0.78mmol) of mono-6-p-toluenesulfonyl cyclodextrin, 0.80g (4.22mmol) of dopamine hydrochloride, add catalyst DMAP to 0.19g (1.5mmol), KI to 0.06g (0.4mmol), and repeatedly vacuumize And fill with nitrogen, then inject 10mL of anhydrous DMF and 1mL of anhydrous methanol into the needle, d...

Embodiment 2

[0036] According to the reaction method of Example 1, the molecular weight of hyaluronic acid was changed to study the influence of molecular weight on the mechanical strength of the hydraulic membrane. When the molecular weight of hyaluronic acid varies from 400kDa to 1300kDa, its influence on the stress-strain value of the hydrogel membrane is verified.

[0037] Table 4 Effect of crosslinking agent content on hydrogel film stress-strain value

[0038] Molecular weight (kDa)

[0039] It can be seen from Table 4 that the molecular weight of hyaluronic acid has an optimal range. To prepare a hydrogel film similar to the elasticity and toughness of the skin epidermis, an appropriate molecular weight must be provided. When the molecular weight of hyaluronic acid is too small, the crosslinking strength is not enough. , causing the stress and strain of the gel film to be small, which cannot match the elastic strain value of the skin. Therefore, it can be known from experi...

Embodiment 3

[0041] According to the reaction method of Example 1, change the molar ratio of HA and β-CD-6-DA reaction substrate, when the reaction molar ratio of HA and β-CD-6-DA is respectively 1:1, 2:1, 4 :1 and 8:1, the hydrogel films prepared were denoted as HA-DA-HRP-1, HA-DA-HRP-2, HA-DA-HRP-3, and HA-DA-HRP-4, respectively. Then use a universal testing machine to test the tensile elongation at break of the four film materials, commercial 3M hydrogel and wound plaster. Test results such as figure 1 shown.

[0042] Depend on figure 1 The results show that HA-DA-HRP-2 and HA-DA-HRP-3 are greater than the elongation at break (140-180%) of human skin stretching, but the tensile stress at break is in the range of 0.50-1.85MPa, indicating that The films prepared by HA-DA-HRP-2 and HA-DA-HRP-3 have the same tensile strength as the skin epidermis, that is, HA-DA-HRP-2 and HA-DA-HRP-3 films It has the function of skin stretch response. However, the tensile strength of commercial 3M hydr...

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Abstract

The invention discloses a preparation method of a hydrogel film with high adhesion to the skin and a function of promoting wound healing. The method is characterized by comprising the steps of: adding1-ethyl-(3-dimethyl aminopropyl) carbodiimide hydrochloride and 1-hydroxybenzotriazole to a 1% hyaluronic acid aqueous solution, adjusting the pH value of a reaction system, performing activation inice water bath for 2h, adding mono-6-deoxy-dopamine cyclodextrin derivative to an obtained solution, performing uniform mixing with stirring, adjusting pH value, performing reaction at 50 DEG C for 48-120h, performing reduced-pressure distilling to remove solvent, adding horseradish catalase and 30% hydrogen peroxide for cross-linking reaction, performing standing overnight, dialysing small molecule catalyst and unreacted substrate, then performing film casting, and volatilizing moisture to obtain the hydrogel film. The hydrogel film prepared by the method has excellent biocompatibility and good gas permeability, and can load drugs to synergistically promote wound healing, and particularly has a stress strain range corresponding to skin tissue under the disturbance of external environmentand stress reaction in the body.

Description

technical field [0001] The invention relates to the technical field of hydrogel membranes, in particular to a preparation method of a hydrogel membrane that is highly compatible with skin and can promote wound healing. Background technique [0002] Hydrogel is a high molecular polymer with water as the dispersed phase containing hydrophilic group molecules. Since the hydrophilic group molecules can generate hydrogen bond interactions with water molecules, the hydrogel has a high degree of water absorption and water retention. Even a water content of more than 90% will make the hydrogel have elasticity and toughness similar to human tissue, so the hydrogel has a wide range of applications in wound dressing. [0003] Hydrogels with better water retention can prevent wound drying, infection, absorb wound exudate, reduce bacterial contamination, transport soluble drugs, and prevent secondary mechanical damage to wounds. Therefore, people use hydrogels He has great research inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/36A61K47/40A61P17/02
CPCA61K9/7007A61K47/36A61K47/40A61P17/02
Inventor 杨成张冬梅
Owner JIANGNAN UNIV
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