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Vitamin B2 modified IONzyme, and preparation method and application thereof

A vitamin and nano-enzyme technology, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of no modified iron-based nano-enzymes, etc., and achieve the reduction of ROS scavenging ability, Eco-friendly, environmentally friendly effect

Active Publication Date: 2019-04-12
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in the prior art, no vitamin B 2 Related reports on modification of iron-based nanozymes
Iron-based nanozymes and vitamin B 2 Combined, which activities can be shown, and whether it has an impact on the activity of iron-based nanozymes, no relevant conclusions have been given in the prior art

Method used

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  • Vitamin B2 modified IONzyme, and preparation method and application thereof
  • Vitamin B2 modified IONzyme, and preparation method and application thereof
  • Vitamin B2 modified IONzyme, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Vitamin B 2 Preparation method of modified iron-based nanoenzyme

[0052] Dissolve 1.35 g of ferric chloride hexahydrate (0.005 mol) in 40 ml of ethylene glycol and stir for 30 minutes with a magnetic stirrer to fully dissolve ferric chloride hexahydrate to prepare a ferric chloride solution. Add 3.6 g of anhydrous sodium acetate to the above ferric chloride solution, and stir with a magnetic stirrer for 30 minutes to fully dissolve the anhydrous sodium acetate to prepare a mixed solution. Add 0.5g of vitamin B to the above mixed solution 2 (0.0013mol), stir for 30 minutes, make vitamin B 2 Mix thoroughly to prepare a reaction solution. Then pour all the reaction liquid into the reaction kettle, put the reaction kettle into the oven, then heat the oven to 200°C, keep it warm and heat for 12 hours, wash with alcohol three times, and dry it to obtain vitamin B. 2 The modified iron-based nanozyme was 0.3g, and the yield was 33.9%.

[0053] In this manual, it also pro...

Embodiment 2

[0055] Example 2 Vitamin B 2 Structural characterization of modified iron-based nanozymes

[0056] Vitamin B 2 The structural characterization of the modified iron-based nanozyme is as Figure 1-5 Shown, where, Figure 1-5 Respectively, 0.5g vitamin B added in Example 1 2 VB obtained 2 -IONzyme's scanning electron microscope image, high resolution transmission electron microscope image, elemental analysis image, X-ray photoelectron spectroscopy analysis image and X-ray diffraction image.

[0057] by figure 1 with figure 2 Available, vitamin B prepared by the above method 2 The modified iron-based nanozyme is irregularly spherical, the particle size is about 200nm, and the particle size is in the nanometer range, indicating that the obtained vitamin B 2 The modified iron-based nanozyme is a nanostructure.

[0058] by image 3 It can be seen that in addition to the peaks of Fe and O elements, there are also peaks of C and N elements in the figure, indicating that after vitamin B 2 C an...

Embodiment 3

[0062] Example 3 Vitamin B 2 Detection of catalase activity of modified iron-based nanozymes

[0063] Vitamin B 2 The method for detecting catalase activity of the modified iron-based nanozyme includes the following steps:

[0064] In the sodium acetate solution system of pH=7.0, add different concentrations of H 2 O 2 Solution, then add the same concentration of vitamin B 2 Modified iron-based nanoenzyme solution, or unmodified iron-based nanoenzyme solution of equal concentration, monitor the dissolved oxygen generated in the system in real time, and perform fitting to obtain H 2 O 2 Concentration-dissolved oxygen generation rate curve. See the results Image 6 .

[0065] Such as Image 6 As shown, under neutral conditions, VB 2 -Both IONzyme and IONzyme can decompose hydrogen peroxide and have catalase activity, but VB 2 -The decomposition rate of IONzyme at each concentration is much higher than that of IONzyme, indicating that VB 2 -IONzyme has higher hydrogen peroxide activity ...

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Abstract

The invention provides a vitamin B2 modified IONzyme, and in particular to vitamin B2 modified nano-scale ferriferrous oxide particles. The invention further provides a preparation method of a vitaminB2 modified IONzyme and a composition for promoting oral ulcer healing. The vitamin B2 modified IONzyme provided by the invention has higher catalase activity and superoxide dismutase activities thanthe IONzyme; and compared with the individually used vitamin B2 and IONzyme, the vitamin B2 modified IONzyme presents better protection effect for BALB / 3T3 cells and HOK cells in a hydrogen peroxideenvironment, better ROS clearance at a cellular level, and better ability to reduce IFN-gamma and IL-6 in ulcers. The vitamin B2 modified IONzyme has a better therapeutic effect on oral ulcers and canbe used to prepare effective drugs for promoting the healing of oral ulcers.

Description

Technical field [0001] This application relates to the technical field of bio-nano materials, in particular to a vitamin B 2 The modified iron-based nanoenzyme and its preparation method and application. Background technique [0002] Iron-based nanozymes (IONzyme) are a class of mimic enzymes that have the unique properties of nanomaterials and catalytic functions. At present, experiments have found that iron-based nanozymes have both catalase (CAT) activity and superoxide dismutase (SOD) activity, and have a series of advantages such as simple preparation, low cost, stability and easy storage. [0003] Oral ulcer, commonly known as "aphthous sore", is a common ulcerative injury disease that occurs in the oral mucosa. It is more common in the inner lip, tongue, belly of the tongue, buccal mucosa, vestibular sulcus, soft palate, etc. The mucosa in these parts lacks keratinized layer Or poor keratinization. Tongue ulcers refer to oral ulcers that occur on the tongue and abdomen of ...

Claims

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Application Information

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IPC IPC(8): A61K47/55A61K33/26A61K31/525A61P17/02A61P1/02
CPCA61K47/551A61P1/02A61P17/02A61K31/525A61K33/26A61K2300/00
Inventor 高利增顾云浩仇智月李丹丹
Owner YANGZHOU UNIV
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