Gynecological tumor cell combined quantitative detection card, preparation method thereof, and using method thereof
A quantitative detection technology for gynecological tumors, applied in measuring devices, scientific instruments, biological tests, etc., can solve the problems of inability to accurately determine whether there is metastasis, inability to achieve real-time dynamic monitoring, inability to reflect heterogeneity, etc., to achieve detection time Short, easy to popularize and use, and increase the effect of response sensitivity
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Embodiment 1
[0071] A combined quantitative detection card for gynecological tumor cells, comprising a bottom card and a face card, the bottom card is provided with: a sample loading area, a binding area, a microchannel, a time valve, a detection area and a waste liquid area, the sample loading area Located at one end of the microchannel, the binding area is connected to a plurality of detection areas through the microchannel and a time valve and located at the other end of the microchannel, and the waste liquid area is connected to the sample loading area through the microchannel;
[0072] There are multiple binding regions.
[0073] The binding area A is connected to the detection areas A1, A2, A3, A4, A5, and A6 through microchannels and time valves;
[0074] The binding area B is connected to the detection areas B1, B2, and B3 through microchannels and time valves;
[0075] The binding area C is connected to the detection areas C1 and C2 through a microchannel and a time valve.
[00...
Embodiment 2
[0101] A method for preparing a combined quantitative detection card for gynecological tumor cells, comprising the following steps:
[0102] (1) Preparation of bottom card:
[0103] (1-1) Drawing: draw the mask layout through the drawing software according to the design of the microchannel pattern;
[0104] (1-2) Mask: After the mask layout is made, print it on a transparent film to make a photolithography mask plate, and use this mask plate to make a silicon wafer mold through a photolithography process;
[0105] (1-3) Photolithography: plate a layer of barrier layer on the surface of the substrate, and then evenly throw a layer of photosensitive material-photoresist on the barrier layer with a glue machine; prepare the required film on the photomask Channel pattern; cover the photomask on the substrate, irradiate the substrate coated with photoresist with ultraviolet light, and the photoresist undergoes photochemical reaction; remove the exposed photoresist by chemical meth...
Embodiment 3
[0113] The method for using the combined quantitative detection card for gynecological tumor cells comprises the following steps:
[0114] (a) The aqueous solution A containing dextran, trehalose and gelatin was added to the aqueous solution B containing polyethylene glycol to form a fibril network, and then 1 wt% egg white lysozyme was added for heat incubation at 60°C, the fibrils The network is transformed into mature fibrils, and finally the serum standard is added to it, and the mature fibrils are used to wrap the serum standard, and water-in-water micro-droplets with a diameter of 8 μm can be prepared and prepared into a series of concentrations of more than 5 , use several detection cards of the same batch to detect the standard solution of each concentration, take the fluorescence intensity of the detection area as the ordinate, and the concentration of the serum standard solution as the abscissa, draw a standard curve and save the standard curve in the multi-color fluo...
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