Improvement method of quick test paper of helicobacter pylori

Abstract

The invention provides an improvement method of quick test paper of helicobacter pylori. The method comprises the following steps: orderly connecting a sample absorbing pad, a nitrocellulose membrane,and a water-absorbing layer from left to right, and fixing on a bottom plate, wherein a first detection line, a second detection line and a control line C which are mutually separated are coated on the nitrocellulose membrane, the first detection line is the colloidal gold marked rat-anti urease antibody, the second detection line is the colloidal gold marked rat-anti heat shock protein B antibody, and the control line C is the colloidal gold marked goat-anti-rat polyclonal antibody; wrapping the bottom plate, the sample absorbing pad, the nitrocellulose membrane and the water absorbing layerby using a plastic shell, and forming a sample injection hole on the plastic shell; dropping the saliva on the sample absorbing pad through the sample injection hole; preparing the sealed 5ml treating bottle and 3ml suckers, wherein 2ml of treating liquid is held in the treating bottle, and the treating liquid contains 30mg / L of ambroxol hydrochloride, 10g / L of phosphate-tween 20 buffer solutionof sodium bicarbonate.

Description

technical field [0001] The invention relates to a detection test paper, in particular to an improved rapid detection test paper for Helicobacter pylori and an improved method thereof. Background technique [0002] Immunochromatography is a rapid immunoassay technique developed in the 1980s. The basic principle of immunochromatography is that the sample to be detected moves on a membrane made of strip fibers (such as nitrocellulose membrane, glass fiber, etc.) through capillary action, and the analyte and a certain area of ​​the membrane Ligands (generally antigens, antibodies, nucleic acids, etc.) are combined, and then through enzymatic color reaction or direct use of colored markers, intuitive results can be obtained in a short time. Divided by principle, immunochromatography can be divided into two categories, one is based on enzymatic reaction color development, quantified according to color brightness; the other is through coloring markers (such as quantum dots, colloi...

AI Technical Summary

Problems solved by technology

[0005] Existing common methods for detecting Helicobacter pylori, such as carbon-14 breath test, require special equipment, waste needs special treatment, and contains radiation, which is not suitable for children and pregnant women, and cannot be self-examined

Saliva PCR technology requires special equipment and is completed in the laboratory, which takes a long time and costs high, and cannot be self-examined

Helicobacter pylori IgG series antibody serological test uses blood as a sample and needs to be completed in a laboratory. The test results cannot determine whether it is a current infection or a past infection. It is not suitable for re-examination after treatment and cannot be self-examination

The fecal Helicobacter pylori antigen detection method uses feces as a sample, which is inconvenient to collect and needs to be completed in the laboratory. Self-examination is not possible, and it is easy to cause physical and psychological discomfort

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