Rapid culture method of chrysophyceae

A culture method and technology of golden algae, applied in the field of rapid cultivation of golden algae, can solve the problems of low culture density, slow growth rate of culture, difficulty in cultivating golden algae biomass to meet the requirements of industrial processing and application, and achieve energy saving, The effect of stable efficiency

Pending Publication Date: 2019-05-28
德默特生物科技(珠海)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the growth rate of photosynthetic culture of golden algae is very slow, and the culture density is also very low, so it is difficult to quickly obtain golden algae biomass that can be pro

Method used

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  • Rapid culture method of chrysophyceae
  • Rapid culture method of chrysophyceae
  • Rapid culture method of chrysophyceae

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0056] Example 1

[0057] Cultivation of golden algae in a 7.5 L, New Brunswick (NBS) BioFlo310 fermentor.

[0058] Using this invention method to cultivate in a 7.5L fermentor, the highest cell number can reach 2.88×10 8 Cell biomass can reach 56g / L per mL.

[0059] Step 1: Photoautotrophic pre-cultivation of seeds

[0060] (1) Prepare the seed culture medium according to Table 1, and dispense them into 1L Erlenmeyer flasks with a volume of 300mL and sterilize at 121°C for 30 min.

[0061] (2) Inoculation, algae species Poterioochromonas malhamensis CMBB-01 was provided by the Institute of Hydrology, Wuhan Chinese Academy of Sciences, with an inoculation volume of 10 mL.

[0062] (3) Cultivate for 7 days, culture temperature 25-28 ℃, light intensity 30-50 μmol photons m -2 s -1 . When the seed color changes from golden yellow to dark brown, the dry weight can reach 2-3g / L.

[0063] Step 2: Fermentation tank culture

[0064] (1) Prepare the fermentation tank culture medium according to ...

Example Embodiment

[0078] Example 2

[0079] Cultivation of golden algae in 125 L, New Brunswick (NBS) BioFlo610 type fermentor.

[0080] Using the method of the invention to carry out amplifying culture on a 125L fermentor, the cell biomass of Chrysophyllum algae can reach 45 g / L after 96 hours of culture.

[0081] Step 1: Seed preparation

[0082] After culturing for 96h in Example 1, figure 1 It is known that it is in the logarithmic phase and is suitable for vaccination. The golden algae at this time was taken out and used as the algae seed of this example.

[0083] Step 2: Fermentation tank culture

[0084] (1) Prepare the fermentation tank culture medium according to Table 2 (except ammonium chloride), calibrate the pH electrode, prepare 32 L per tank, fill 28L, 121 ℃, and sterilize for 30 min. The mass ratio of carbon / nitrogen in the medium is 28:1.

[0085] (2) After sterilization, cool down, adjust the temperature to 28 ℃, rotate speed 50 rpm, and ventilate 4.0 L min -1 After that, calibrate the ...

Example Embodiment

[0104] Example 3

[0105] According to the cultivation method of Example 1, cultured in a 7.5L fermenter, and set up the experimental group with vitamins (tank 1 and tank 3) and the control group (tank 2 and tank 4) without vitamins, as shown in the following table:

[0106]

[0107] The cultivation method, conditions and operation steps of this example are all carried out with reference to Example 1. The experimental results are shown in image 3 . by image 3 The statistical results show that:

[0108] ①By image 3 It can be seen that tank 1 and tank 3 add vitamin B in 44 h 1 :0.375 mg / L, B H :0.25mg / L, B 6 :0.125 mg / L and B 12 : 0.125 mg / L (the concentration of vitamins after adding the culture system), the growth rate began to be significantly faster than tank 2 and tank 4. The average rate of the fermentation tank of tank 1 is 32.22×10 in 48-120 h 5 (Each per mL / h), the average speed of tank 2 fermentor is 7.4×10 5 (A mL -1 h -1 ), tank 1 is 4.4 times the cell growth rate of t...

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Abstract

The invention relates to a rapid culture method of chrysophyceae. The culture method is heterotrophic culture; a culture medium contains a carbon source and a nitrogen source; the carbon source is glucose; the nitrogen source is a mixed carbon source composed of an organic nitrogen source and an inorganic nitrogen source; the organic nitrogen source is selected from one or more of liver extract powder, beef extract, yeast extract powder and peptone; the inorganic nitrogen source is ammonium nitrogen or urea. In a culture process, a material supplementation culture medium is supplemented into afermentation tank; the temperature of a culture system in the fermentation tank is controlled to be 20 to 30 DEG C, the pH (Potential of Hydrogen) is 5.0 to 8.0, the dissolved oxygen concentration is10 to 60 percent and the concentration of glucose is stabilized to be 0 to 2g/L; a nitrogen-containing material supplementation culture medium is supplemented from the beginning of the culture to a first moment, and a nitrogen-free material supplementation culture medium is supplemented from the first moment to a second moment or the ending of the culture; a basic culture medium contains vitaminB1, BH, B6 or B12 or the vitamin B1, BH, B6 or B12 is added into the fermentation tank in a culture process. According to the method provided by the invention, after the culture is carried out for 96to 170h, the highest biomass can reach 56g/L and the growth of the chrysophyceae is remarkably accelerated after the vitamin B1, BH, B6 or B12 is added.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a rapid cultivation method for golden algae. Background technique [0002] Golden algae, also known as golden brown algae, are mostly distributed in fresh water and also in sea water. Golden algae cells are rich in nutrients, among which golden algae laminarose has a wide range of biological activities, and has a certain preventive effect on gout, hyperlipidemia, kidney disease, and early and middle stage renal failure; and the fucoxanthin contained in golden algae has multiple Biological effects, including weight loss, anti-oxidation, neuroprotection, anti-tumor, anti-inflammation, hypoglycemic / lipidemia, liver protection, anti-angiogenesis, eye protection and other pharmacological effects, so it is used as medicine, skin care and beauty products and health care products widely used in the market. Golden algae is also a good fish bait and has a wide range of use...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 陈剑平马明洋王谝龚迎春金虎胡强
Owner 德默特生物科技(珠海)有限公司
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