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Novel nerve duct bracket and preparation method and application thereof

A nerve conduit, a new type of technology, applied in the field of biomedical materials, can solve the problems of inability to repair long-distance nerve defects, lack of good guidance for nerve regeneration, and lack of biological activity. It achieves simple and easy preparation methods, safe and reliable raw materials, Good biocompatibility effect

Active Publication Date: 2019-06-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a novel nerve conduit stent and its preparation method and application, to overcome the lack of biological activity, permeability and mechanical properties of the existing two-dimensional nerve conduit stent, so that it does not guide nerve regeneration well, Failure to repair long-distance nerve deficits

Method used

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  • Novel nerve duct bracket and preparation method and application thereof
  • Novel nerve duct bracket and preparation method and application thereof
  • Novel nerve duct bracket and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0039] (1) Dissolve 10g of gelatin and 4g of PLA in 10ml of hexafluoroisopropanol, and keep stirring until completely dissolved to obtain a spinning solution with a concentration of 10% (mass volume ratio); add 3mg of redox graphene to the above Ultrasonic dispersion in the spinning solution to obtain the spinning solution of graphene / gelatin / PLA, spinning conditions: voltage 13 kV, receiving distance 10cm, spinning rate 1.4ml / h, electrospinning to obtain nanofiber membrane, placed in Store in a vacuum oven for later use.

[0040] (2) Shred the nanofiber membrane obtained in step (1), weigh 2g of the fragments and add them to a beaker filled with 10ml of tert-butanol, and use a high-speed homogenizer to carry out homogenization and pulverization of the nanofiber membrane fragments, Until the fiber membrane is completely broken and evenly dispersed in the tert-butanol solution. The uniformly dispersed nanofiber mixture is poured into a multi-channel mold and freeze-dried to ob...

Embodiment 2

[0043] (1) 10g of gelatin is dissolved in 10ml of hexafluoroisopropanol, and it is continuously stirred until fully dissolved to obtain a gelatin solution with a concentration of 12% (mass volume ratio); 6g of PCL is dissolved in hexafluoroisopropanol, and Constantly stir until completely dissolving, obtain the PCL solution liquid that concentration is 8% (mass volume ratio); 10ml above-mentioned gelatin solution is mixed with 5ml above-mentioned PCL solution respectively, stir, obtain the spinning solution of gelatin / PCL; 4mg graphite Graphene is added to the above-mentioned spinning solution for ultrasonic dispersion to obtain a spinning solution of graphene / gelatin / PCL, spinning conditions: voltage 10 kV, receiving distance 10cm, spinning rate 1.2ml / h, electrospinning to obtain nanofiber membranes , stored in a vacuum oven for later use.

[0044] (2) Shred the nanofiber membrane obtained in step (1), weigh 0.5g of the fragments and add them to a beaker filled with 10ml of t...

Embodiment 3

[0047] (1) Place the silk at 95°C in a solution containing 5g / L Na 2 CO 3 The degumming solution was degummed 3 times, each time for 30 minutes, and the bath ratio was 1:50. After degumming, the tussah silk fibers are obtained and dried at 60°C. Put the tussah silk fibroin fiber in a saturated LiSCN solution at a bath ratio of 1:10, and dissolve it at 50°C±2°C for 70 minutes. Dialyzed with deionized water for 3 days, and freeze-dried to obtain tussah silk fibroin.

[0048] (2) Weigh 0.8g of silk fibroin obtained in step (1), dissolve 0.2g of PLCL in 10ml of hexafluoroisopropanol, stir until completely dissolved, and obtain a spinning solution with a concentration of 10% (w / v), Add 2mg of graphene oxide into the spinning solution and ultrasonically disperse to prepare the spinning solution of graphene oxide / silk fibroin / PLCL. Electrospin the spinning liquid, spinning conditions: voltage 14 kV, receiving distance 10cm, spinning rate 1.0ml / h, receive a certain thickness of na...

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Abstract

The invention relates to a novel nerve duct bracket and a preparation method and application thereof. Three-dimensional graphene or graphene derivative multichannel active nanofiber sponge is used asa stuffing inner core, and nanofibers in an axial direction are used as an outer pipe. The preparation method comprises the steps of performing ultrasonic dispersion on graphene or a graphene derivative into spinning liquid, performing electrostatic spinning, and performing drying so as to obtain a nanofiber film; performing shearing, and performing homogenization and crushing so as to obtain broken fiber mixed liquid; pouring the broken fiber mixed liquid into a multichannel mold, and performing freeze drying; performing cross-linked shaping; performing drying and shaping so as to obtain thethree-dimensional graphene or graphene derivative multichannel nanofiber sponge; and performing fixing in an axial direction spinning receiver, and performing axial direction spinning on an outer layer so as to obtain the novel nerve duct bracket. The novel nerve duct bracket is used for inducing repair and regeneration of nerve tissue. The novel nerve duct bracket disclosed by the invention is simple to operate and good in repeatability, and a new thinking is provided for repairing neurological deficits.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a novel nerve guide bracket and its preparation method and application. Background technique [0002] Peripheral nerve injury is a common clinical disease with poor prognosis and high disability rate, which brings huge economic losses and mental burden to patients, families and society [Hudson et al, 2000, Lundborg et al, 1982, Zhang et al ,2017]. About 2 million cases of this kind of nerve injury occur every year in our country. Nowadays, microsurgical techniques are often used to perform end-to-end adventitial suture, perineural suture or combined suture surgery for the severed injury and small defect of peripheral nerve clinically, in order to restore the continuity of the nerve; however, for larger For nerve defects, autologous transplantation is still the current clinical "gold standard" for repair [Pulley et al, 2016]. However, autologous transpl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/54A61L27/08A61L27/56D04H1/728D01D5/00
Inventor 莫秀梅王娟孙彬彬朱同贺叶凯强沈威朱晶晶
Owner DONGHUA UNIV
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