A kind of amphiphilic polysaccharide derivative containing cholesterol and plant lectin group and its preparation method and application
A technology of amphiphilic polysaccharides and plant lectins, applied in the field of amphiphilic polysaccharide derivatives and their preparation, can solve the problems of difficult subcutaneous or intravenous injection, large size, etc., and achieve cheap equipment and raw materials, convenient operation, and preparation The effect of mild conditions
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Embodiment 1
[0075] 1. Synthesis of amphipathic amylose derivatives containing cholesterol and lectin groups
[0076] (1) Weigh 0.1 g of carboxylated amylose derivatives, dissolve in 20 ml of dimethyl sulfoxide, stir and dissolve in an oil bath at 15° C. for 2 hours;
[0077] (2) Weigh 0.2 g of cholesterol in 10 ml of ethanol, slowly add it dropwise to the solution of step (1), then weigh 0.5 g of DCC and 1.1 g of DMAP, dissolve it in 100 ml of organic solvent, and slowly add it dropwise to step (1) ) solution, continue to stir and react in an oil bath at 15°C for 1 hour;
[0078] (3) After the above reaction was completed, 1 mL of acetone was added dropwise to the reaction solution to precipitate amphipathic amylose derivatives containing cholesterol, and the precipitate was collected by centrifugation (5000 rpm, 5 min). Dissolve the precipitate in 20 mL of ethanol, add 1-20 mL of acetone dropwise to precipitate amphipathic amylose derivatives containing cholesterol, centrifuge (5000 rpm...
Embodiment 2
[0099] 1. Synthesis of amphiphilic glycogen derivatives containing cholesterol and lectin groups
[0100] (1) Weigh 0.3 g of carboxylated glycogen derivatives, dissolve in 15 ml of acetonitrile, stir and dissolve in an oil bath at 40° C. for 5 hours;
[0101] (2) Weigh 0.2 g of cholesterol in 10 ml of acetone, slowly add it dropwise to the solution of step (1), then weigh 1.0 g of DCC and 1.0 g of DMAP, dissolve it in 100 ml of acetone, and slowly add dropwise to the solution of step (1) solution, the stirring reaction was continued for 5 hours.
[0102] (3) After the above reaction was completed, 1 mL of acetone was added dropwise to the reaction solution to precipitate amphiphilic glycogen derivatives containing cholesterol, and the precipitate was collected by centrifugation (5000 rpm, 5 min). Dissolve the precipitate in 20 mL of ethanol, add 1-20 mL of acetone dropwise to precipitate amphiphilic glycogen derivatives containing cholesterol, centrifuge (5000 rpm, 5 min), an...
Embodiment 3
[0115] 1. Synthesis of amphiphilic guar gum derivatives containing cholesterol and lectin groups
[0116] (1) Weigh 0.5 g of carboxylated guar gum derivatives, dissolve in 15 ml of formamide, stir and dissolve in an oil bath at 20° C. for 20 hours;
[0117] (2) Weigh 0.5 gram of cholesterol in 15 milliliters of dimethylformamide, slowly add it dropwise to the solution of step (1), then weigh 0.5 gram of DCC and 1.5 gram of DMAP and dissolve in 100 milliliters of dimethylformamide, Slowly added dropwise to the solution of step (1), and continued to stir and react for 20 hours.
[0118] (3) After the above reaction was completed, 1 mL of isopropyl ketone was added dropwise to the reaction solution to precipitate amphiphilic glycogen derivatives containing cholesterol, and the precipitate was collected by centrifugation (5000 rpm, 5 min). Dissolve the precipitate in 20 mL of dimethylformamide, add 1-20 mL of isopropyl ketone dropwise to precipitate amphiphilic glycogen derivativ...
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