A method for embedding and immobilizing enzymes in polymer microcapsules
A technology for immobilizing enzymes and polymers, which is applied in the direction of immobilizing enzymes, biochemical equipment and methods, and immobilizing on or in inorganic carriers. It can solve the problems of thin polymer microcapsules and poor stability. Controllable, stable structure, uniform mesh size effect
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Embodiment 1
[0018] Take 10 mL of a mixed solution containing 0.6 mol / L calcium chloride and 3 mg / mL glucose oxidase, stir evenly, add an equal volume of 0.6 mol / L sodium carbonate solution, stir for 5 minutes with magnetic force, centrifuge and wash for later use. Take 0.5 g of calcium carbonate microspheres embedding the enzyme and add it to a polyethyleneimine solution with a mass fraction of 1%. The adsorption reaction was carried out for 20 minutes, and it was washed by centrifugation before use. Take 0.5 g of calcium carbonate microspheres embedded with enzyme after absorbing polyethyleneimine, and 0.05 g of photoinitiator thioxanthone catechol-O,O'-diacetic acid, and add them to polyethyleneimine containing 10% mass fraction. In the alcohol diacrylate (PEGDA molecular weight 575) solution, the volume of the solution is 50 mL, and the solvent is a phosphate buffer solution with pH=7. After sealing the device, pass nitrogen gas, and move into the visible light lamp irradiation device...
Embodiment 2
[0022] Take 10 mL of a mixed solution containing 0.6 mol / L calcium chloride and 3 mg / mL glucose oxidase, stir evenly, add an equal volume of 0.6 mol / L sodium carbonate solution, stir for 5 minutes with magnetic force, centrifuge and wash for later use. Take 0.25 g of enzyme-embedded calcium carbonate microspheres and add it to a polyethyleneimine solution with a mass fraction of 1%. The volume of the solution is 50 mL. The solvent in the polyethyleneimine solution is a phosphate buffer solution with a pH of 7. Magnetic stirring The adsorption reaction was carried out for 20 minutes, and it was washed by centrifugation before use. Take 0.25 g of calcium carbonate microspheres embedded with enzyme after absorbing polyethyleneimine, and 0.05 g of photoinitiator thioxanthone catechol-O,O'-diacetic acid, and add them to polyethyleneimine containing 10% mass fraction. In the alcohol diacrylate (PEGDA molecular weight 575) solution, the volume of the solution is 50 mL, and the solven...
Embodiment 3
[0026] Take 10 mL of a mixed solution containing 0.4 mol / L calcium chloride and 1 mg / mL glucose oxidase, stir evenly, add an equal volume of 0.4 mol / L sodium carbonate solution, stir for 5 minutes with magnetic force, centrifuge and wash for later use. Take 0.5 g of calcium carbonate microspheres embedding the enzyme and add it to a polyethyleneimine solution with a mass fraction of 1%. The adsorption reaction was carried out for 10 minutes, and it was washed by centrifugation before use. Take 0.25 g of calcium carbonate microspheres embedded with enzyme after absorbing polyethyleneimine, and 0.03 g of photoinitiator thioxanthone catechol-O, O′-diacetic acid, and add them to polyethyleneimine containing 10% by mass. Alcohol diacrylate (PEGDA molecular weight 575) and 0.01% by mass of methylenebisacrylamide solution, the volume of the solution is 50 mL, and the solvent is phosphate buffer with pH=7. After the device is sealed, nitrogen gas is passed, and it is moved into a vis...
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