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Method for producing high-protein feed by solid fermentation of soybean meal

A high-protein feed and solid fermentation technology, which is applied in the field of high-protein production of solid fermented soybean meal, can solve problems such as affecting the digestion, absorption and metabolism of animal nutrients, difficulty in utilizing high-quality protein resources, and adding time affecting enzymatic hydrolysis effects, etc. Absorption and utilization, easy for animals to digest and absorb, beneficial to growth, reproduction and metabolism

Pending Publication Date: 2019-07-05
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the fact that soybean meal contains various anti-nutritional factors, the active feed peptide produced by simple solid fermentation still has a high content of anti-nutritional factors, which seriously affects the utilization of protein in soybean meal and the survival of bacteria. At present, only about 40% of the protein in soybean meal can be enzymatically hydrolyzed on the market, and the number of viable bacteria is relatively low, which seriously affects the digestion, absorption and metabolism of the nutrients in the protein feed fermented by soybean meal, so that pure solid-state fermentation There are problems of low digestibility and biological potency, making it difficult to utilize high-quality protein resources in soybean meal
[0003] At present, there are patents that disclose the joint fermentation of mixed bacteria to eliminate anti-nutritional factors, but there are mutual influences between various bacteria, and the stability of the obtained product is difficult to guarantee. The dosage requirements are relatively high, and different enzyme dosages, adding time, and action temperature will directly affect the enzymolysis effect. Many patents directly mix different enzymes into the strains to ferment soybean meal together, but due to the limitation of microbial fermentation conditions , it is difficult to make the enzyme fully play a role in assisting the bacteria to eliminate anti-nutritional factors, so the synergistic effect of the bacteria and enzymes is low, and the problem of low conversion rate of soybean meal protein still exists

Method used

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  • Method for producing high-protein feed by solid fermentation of soybean meal
  • Method for producing high-protein feed by solid fermentation of soybean meal
  • Method for producing high-protein feed by solid fermentation of soybean meal

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Step 1: Bacillus subtilis was inoculated into the liquid medium at a mass percentage of 3%, and cultured at 32°C for 68 hours to obtain a fermentation broth; the bacterial content of the fermentation broth was: 8*10 8 cfu / mL, the liquid medium contains the following components by weight: 3 parts of yeast extract, 10 parts of corn yellow powder, 3 parts of dipotassium hydrogen phosphate, 0.1 part of trace element group and 800 parts of water; the trace element group contains the following Components in parts by weight: 0.01 part of magnesium sulfate, 0.01 part of zinc sulfate, 0.01 part of ferric chloride, 0.01 part of cobalt chloride and 0.01 part of copper sulfate;

[0058] Step 2: After the soybean meal was sterilized at 105°C for 30 minutes, papain was added at 3.5% by mass, and sterile water was added to keep the moisture content of the sterile soybean meal at 50%, and enzymatic hydrolysis was performed at 40°C for 30 minutes to obtain enzymatic hydrolysis product; ...

Embodiment 2

[0062] Step 1: Bacillus subtilis was inoculated into a liquid medium at 4% by mass, and cultured at 34°C for 70 hours to obtain a fermentation broth; the bacterial content of the fermentation broth was: 8.8*10 8 cfu / mL, the liquid medium contains the following components by weight: 5 parts of yeast extract, 12 parts of corn yellow powder, 4 parts of dipotassium hydrogen phosphate, 0.15 parts of trace element group and 1000 parts of water; the trace element group contains the following Components in parts by weight: 0.02 part of magnesium sulfate, 0.02 part of zinc sulfate, 0.02 part of ferric chloride, 0.01 part of cobalt chloride and 0.015 part of copper sulfate;

[0063] Step 2: After the soybean meal is sterilized at 105°C for 30 minutes, papain is added at 4% by mass, and sterile water is added to keep the moisture content of the sterile soybean meal at 50%, and enzymatically hydrolyzed at 50°C for 35 minutes to obtain enzymatic hydrolysis product;

[0064] Step 3: After ...

Embodiment 3

[0067] Step 1: Bacillus subtilis was inoculated into the liquid medium at 5% by mass, and cultured at 36°C for 72 hours to obtain a fermentation broth; the bacterial content of the fermentation broth was: 10*10 8 cfu / mL, the liquid medium contains the following components by weight: 8 parts of yeast extract, 15 parts of corn yellow powder, 5 parts of dipotassium hydrogen phosphate, 0.3 parts of trace element group and 1000 parts of water; the trace element group contains the following Components in parts by weight: 0.03 part of magnesium sulfate, 0.02 part of zinc sulfate, 0.02 part of ferric chloride, 0.015 part of cobalt chloride and 0.015 part of copper sulfate;

[0068] Step 2: After the soybean meal was sterilized at 105°C for 30 minutes, papain was added at 4.5% by mass, and sterile water was added to keep the moisture content of the sterile soybean meal at 60%, and enzymatically hydrolyzed at 50°C for 40 minutes to obtain enzymatic hydrolysis product;

[0069] Step 3: ...

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Abstract

The invention discloses a method for producing high-protein feed by solid fermentation of soybean meal. The method comprises the following steps: step 1, fermenting bacillus subtilis liquid to obtaina fermentation liquor; step 2, after the soybean meal is sterilized at high temperature, adding 3.5-4.5% by mass of papain, and performing enzymolysis for 30-40 min at the temperature of 40-50 DEG C to obtain an enzymolysis product; step 3, inoculating 3-5% by mass of the fermentation liquor into the enzymolysis product, adding 10-20 U of neutral protease and 15-20 U of acidic protease into each gram of soybean meal, uniformly mixing to prepare a fermentation raw material, performing solid-state fermentation for 60-72 h at the temperature of 30-36 DEG C, and drying at low temperature to obtainthe high-protein feed. According to the invention, by adding different proteases at different time periods, the synergistic effect of bacterial enzymes can be fully coordinated, so that elimination of anti-nutritional factors and survival of strains are promoted, the utilization rate of protein in the soybean meal is improved, and soluble protein reaches up to 60%.

Description

technical field [0001] The invention relates to the technical field of feed production, in particular to a method for producing high protein from solid fermented soybean meal. Background technique [0002] Soybean meal is a by-product obtained from soybean extraction of soybean oil, also known as soybean meal. The crude protein content in soybean meal can reach 30-50%, which can provide various amino acids for animals and is the main source of vegetable protein feed. The content and quality of protein in the feed is an important criterion for evaluating its quality, and it is also directly related to the health and production level of animals. At present, the methods for large-scale production of feed from soybean meal mainly include: microbial fermentation and enzymatic hydrolysis. Among them, the enzymatic hydrolysis method can produce specific peptides or amino acids by positioning specific enzymes, the enzymatic hydrolysis process and products are easy to control, the ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K10/14
CPCA23K10/12A23K10/14A23K10/37Y02P60/87
Inventor 彭楠常章兵田建平梁运祥
Owner HUAZHONG AGRI UNIV
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