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Mass spectrometry imaging method for simultaneously analyzing various amine metabolites in biological tissues

A mass spectrometry imaging and biological tissue technology, applied in the field of mass spectrometry detection, can solve the problems of insufficient sensitivity, strong aggregation peaks, affecting the spectral imaging analysis of target amine metabolites, etc., and achieves high sensitivity, simple operation and good detection reproducibility. Effect

Active Publication Date: 2019-07-16
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although MALDI can achieve in situ analysis of lipids, peptides, proteins, etc. in tissues at a spatial resolution of several microns, the inventors found that due to the insufficient sensitivity of MALDI mass spectrometry imaging technology and the need to spray matrix on tissue sections during the sample preparation stage , it is easy to cause very strong polymerization peaks in the low-mass region (m / z<700), which seriously affects the mass spectrometry imaging analysis of target amine metabolites
Therefore, how to improve the detection sensitivity and achieve high-coverage in situ analysis of small molecule amine metabolites in biological tissues is still a huge challenge for MALDI mass spectrometry imaging analysis.

Method used

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  • Mass spectrometry imaging method for simultaneously analyzing various amine metabolites in biological tissues
  • Mass spectrometry imaging method for simultaneously analyzing various amine metabolites in biological tissues
  • Mass spectrometry imaging method for simultaneously analyzing various amine metabolites in biological tissues

Examples

Experimental program
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Embodiment 1

[0050] (1) Take fresh rat spleen tissue and use a Thermo CryoStar NX50NOVPD microtome to make 12-micron-thick frozen sections, making 6 slices in total;

[0051] (2) The frozen section is transferred to the ITO-indium tin oxide conductive glass slide of 25mm*75mm with a fiber brush;

[0052] (3) Place the tissue section in a vacuum desiccator, and after drying it under vacuum for 15 minutes, then place the tissue section in a staining jar for standby;

[0053] (4) No treatment reagent was added to one of the tissue slices, and an appropriate amount of ice acetone was added to the staining vat for the other 5 slices, and then the staining vat was placed on a shaker, and the shaking speed of the shaker was set to 60 times / min. Take out the tissue sections from the staining jar soaked in ice acetone at the 1st, 2nd, 5th, 10th, and 15th minutes of shaking, and dry them for later use;

[0054] (5) Precisely weigh 25mg of 1,5-diaminonaphthalene into a 10mL volumetric flask, add 10m...

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Abstract

The invention provides a MALDI mass spectrometry imaging method for simultaneously analyzing various amine metabolites in biological tissues, and belongs to the technical field of mass spectrometry. The various amine metabolites are choline, glycerophosphocholine, carnitine, acetylcarnitine, propionylcarnitine, histamine, spermine, spermidine, betaine, butyrylcarnitine, valerylcarnitine, C18:0 carnitine, acetylcholine, creatine, histidine, phosphorylcholine (a total of 16 kinds). The method is simple in operation, high in sensitivity and good in repeatability, and can be used for visual representation of amine metabolites in biological tissues, thereby having a good value for practical application.

Description

technical field [0001] The invention belongs to the technical field of mass spectrometry detection, and in particular relates to a mass spectrometry imaging method for simultaneously analyzing multiple amine metabolites in biological tissues. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Biological tissue has a very complex structure and function, and it is also the target site for most biological functions and the occurrence and development of diseases. Among them, amine metabolites, as important constituent molecules of biological tissues, play an important role in the formation of cell biofilm, intercellular signal transduction and energy metabolism. In situ analysi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 孙成龙王晓赵恒强马春霞刘伟耿岩玲
Owner SHANDONG ANALYSIS & TEST CENT
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