Preparation method and application of total flavone and total polysaccharide in cyclocarya paliurus leaves

A technology for total flavonoids and total polysaccharides, applied in the field of medicine and food, can solve the problems of difficulty in realizing high-efficiency utilization and industrialization of pennywort resources, low purity of extracts, etc., and achieves simple and easy preparation process steps, low price and high efficiency. Effect

Pending Publication Date: 2019-07-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, most extraction or purification methods are mainly water or organic solvent (such as ethanol) direct extraction or reflux extraction, the purity of the extract is low, and only focus on a single extraction of a component, t...

Method used

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  • Preparation method and application of total flavone and total polysaccharide in cyclocarya paliurus leaves
  • Preparation method and application of total flavone and total polysaccharide in cyclocarya paliurus leaves
  • Preparation method and application of total flavone and total polysaccharide in cyclocarya paliurus leaves

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Effect test

Embodiment 1

[0022] Dry 5 kg of Cyclocarya paliurus leaves, grind into powder, and extract twice with 50 L of 60% ethanol solution at room temperature, each time for 7 days. The alcohol extracts are combined and filtered, the filtrate is used for further separation of total triterpenoids and total flavonoids, and the filter residue is used for further extraction of total polysaccharides. The filtrate was concentrated to dryness under reduced pressure to obtain 780 g of alcohol extract. The alcohol-extracted extract was dispersed with 1 L of water, and extracted successively with equal volumes of petroleum ether and chloroform to obtain 300 g of the chloroform extract. The chloroform extraction part is put on a 60-80 mesh polyamide column, and is sequentially eluted with petroleum ether: acetone = 1:1, acetone, 70% ethanol solution, and the petroleum ether acetone eluate is collected to obtain a crude triterpene extract. The polyamide chromatographic column is then eluted with a mixed solu...

Embodiment 2

[0024] 10 kg of dried Cyclocarya paliurus leaves were powdered and refluxed with 80 L of 70% ethanol solution twice for 2 hours each time. The alcohol extracts are combined and filtered, the filtrate is used for further separation of total triterpenoids and total flavonoids, and the filter residue is used for further extraction of total polysaccharides. The filtrate was concentrated to dryness under reduced pressure to obtain 1600 g of alcohol extract. The alcohol extraction extract was dispersed with 2L of water, extracted successively with equal volumes of petroleum ether and ethyl acetate to obtain 610 g of the ethyl acetate extract. The ethyl acetate extraction part is put on a 100-200 mesh polyamide chromatographic column, and is eluted successively through dichloromethane: acetone = 1:1, acetone, 95% ethanol solution, and the dichloromethane acetone eluate is collected to obtain the crude triterpene extract paste. Collect 95% ethanol eluate to obtain crude flavonoid ex...

Embodiment 3

[0026]Dry 1 kg of Cyclocarya paliurus leaves, powder them, and reflux extract them twice with 10 L of 80% ethanol solution, each time for 100 min. The alcohol extracts are combined and filtered, the filtrate is used for further separation of total triterpenoids and total flavonoids, and the filter residue is used for further extraction of total polysaccharides. The filtrate was concentrated to dryness under reduced pressure to obtain 180 g of alcohol extract. The alcohol extraction extract was dispersed with 500mL of water, extracted successively with equal volumes of petroleum ether, ethyl acetate:n-butanol (10:1), and 75g of the ethyl acetate-n-butanol extraction fraction was obtained. The ethyl acetate n-butanol extraction part is placed on a 100-200 mesh polyamide chromatographic column, eluted with acetone and 95% ethanol solution in sequence, and the acetone eluate is collected to obtain a crude triterpene extract. Collect 95% ethanol eluate to obtain crude flavonoid ex...

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Abstract

The invention provides a preparation method and application of total flavone and total polysaccharide in cyclocarya paliurus leaves. The method comprises the steps of taking cyclocarya paliurus leavesas raw materials, executing extraction by an ethanol-water solvent, executing extraction by an organic solvent, executing elution by a chromatographic column and by macroporous resin to obtain totalflavone, conducting extraction on cyclocarya paliurus leaf dregs by hot water, executing precipitation, removing protein, and adsorbing impurities by macroporous resin to obtain total polysaccharide.Studies show that the total triterpenes, the total flavones, the total polysaccharides and their composition prepared by the method can effectively increase glucose uptake of 3T3-L1 adipocytes, obviously reduce the fasting blood glucose level of the hereditary diabetes db/db mice, has antidiabetic activity, and can be used for preparing products such as antidiabetic medicines, health foods, functional foods and the like for reducing blood sugar. The invention develops the comprehensive preparation method of total flavones and total polysaccharides in cyclocarya paliurus leaves and applicationof the mehtod in resisting diabetes, and provides a basis and a new application direction for popularization, utilization and deep processing of traditional Chinese medicinal materials and new food raw materials.

Description

technical field [0001] The invention belongs to the technical field of medicine and food, and in particular relates to a method for preparing total triterpenes, total flavonoids and total polysaccharides in Cyclocarya paliurus leaves, and the preparation of antidiabetic drugs by using the total triterpenes, total flavonoids and total polysaccharides obtained by the method. , health food and functional food and other products. Background technique [0002] Cyclocarya paliurus leaves are dried leaves of Cyclocarya paliurus (Batalin) Iljinsk. Cyclocarya paliurus is a unique single-species plant in my country, widely distributed in Jiangxi, Zhejiang, Anhui, Hunan, Guangdong and other areas south of the Yangtze River. Folks use its leaves as a tea drink. The tea tastes sweet and has the effects of promoting body fluids and quenching thirst, clearing heat and relieving summer heat, lowering blood sugar, lowering blood pressure and prolonging life. At the end of 2013, green willo...

Claims

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Application Information

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IPC IPC(8): A61K36/52A61P3/10A23L33/105A61K127/00
CPCA23V2002/00A61K36/52A61K2236/331A61K2236/333A61K2236/39A61K2236/53A61K2236/55A23L33/105A61P3/10A23V2200/328A23V2250/21
Inventor 甘礼社周长新方祝君莫建霞杨琪瑶
Owner ZHEJIANG UNIV
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