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Saccharomyces cerevisiae, microbial preparations comprising same, and method for producing ethanol using same

A microbial preparation, the technology of Saccharomyces cerevisiae, applied in the field of microorganisms, can solve the problems of large fluctuations in production performance, unstable ethanol yield, poor fermentation performance, etc., achieve good industrialization and commercial application prospects, reduce production costs, and increase fermentation production Effect

Active Publication Date: 2022-06-28
JILIN COFCO BIOCHEM +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the application of this strain in the ethanol fermentation process (also known as "cellulose ethanol fermentation process") that uses cellulose raw materials such as straw enzymolysis solution as industrial actual materials, there are still fermentations of the strain in the industrial actual material fermentation system. Problems such as poor performance, large fluctuations in production performance due to material differences, and unstable ethanol yield are mainly reflected in the fact that although the recombinant S. poor performance

Method used

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  • Saccharomyces cerevisiae, microbial preparations comprising same, and method for producing ethanol using same
  • Saccharomyces cerevisiae, microbial preparations comprising same, and method for producing ethanol using same
  • Saccharomyces cerevisiae, microbial preparations comprising same, and method for producing ethanol using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1: Preparation of S.C X630 strain

[0057] Taking the C5 / C6 co-fermentation recombinant Saccharomyces cerevisiae strain 0918MUT (referring to patent application CN201711485502.8) that can utilize xylose and glucose in the early stage of genetic engineering as the starting strain, the seed liquid of the starting strain is inoculated with an inoculum of 10v / v% In a test tube containing 5 mL of YEPX liquid medium containing 10 g / L xylose, cultured continuously at 30 °C and 200 rpm, and transferred to 5 mL of fresh medium containing 10 g / L xylose at a volume ratio of 10 v / v% every 72 h. Continue to cultivate in YEPX liquid medium. After 6 times of transfer, apply the culture solution that was continued to cultivate for 72 hours after the sixth transfer on YEPX solid medium, and cultivate at 30°C for 56 hours. Select colonies with a diameter greater than 1 cm. Single colony, four single colonies numbered C-9, C-10, C-11 and C-14 were obtained.

[0058] The single co...

Embodiment 2

[0069] Example 2: Evaluation of fermentation performance of S.C X630

[0070] The fermentation performance of the S.C X630 strain obtained in Example 1 was evaluated by using the actual industrial materials corn stover enzymatic hydrolysate and cassava distiller's grains enzymolysate as raw materials, that is, the changes of the amounts of xylose, glucose and ethanol in the fermentation broth with time were measured. , and calculated parameters such as sugar alcohol conversion rate and xylose utilization rate. The specific process of evaluating the fermentation performance of S.C X630 strain with corn stover enzymatic hydrolyzate as substrate is as follows:

[0071] 1) Seed liquid preparation: Inoculate 1mL-80°C S.C X630 glycerol tube into a shake flask containing 100mL YEPX liquid medium containing 40g / L xylose, cultivate at 30°C and 200rpm for 16h, measure OD 600 ; Transfer 1 mL of culture medium to a 500 mL shake flask containing 200 mL of YEPD liquid medium, and cultivate...

Embodiment 3

[0082] Example 3: Pilot-scale fermentation verification of S.C X630

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Abstract

The invention relates to a strain of Saccharomyces cerevisiae used for ethanol fermentation, a microbial preparation containing it and a method for producing ethanol using the Saccharomyces cerevisiae strain. Based on the recombinant Saccharomyces cerevisiae 0918MUT with the preservation number CGMCC No.15103, the present invention obtains the C5 / C6 sugar co-fermentation Saccharomyces cerevisiae strain S.C X630 which is highly suitable for the current ethanol fermentation materials and processes through a series of acclimation. Using the recombinant Saccharomyces cerevisiae S.C X630 to ferment the cellulosic raw materials used in the industrial production of ethanol, compared with the single fermentation of C6 sugar, can increase the production of ethanol fermentation by about 30%, can save costs, and has good industrial and commercial applications prospect.

Description

technical field [0001] The present invention relates to the technical field of microorganisms. Specifically, the present invention relates to a strain of Saccharomyces cerevisiae for ethanol fermentation, a microbial preparation comprising the same, and its application in the field of ethanol fermentation. Background technique [0002] Biomass energy has been developed as an alternative energy source for fossil fuels such as coal, oil, and natural gas due to its large reserves, wide distribution, and renewable characteristics. important direction. Lignocellulose generally refers to biomass with lignin, cellulose and hemicellulose as the main components. Compared with traditional ethanol production raw materials such as starch and sugar, lignocellulose is very cheap and easy to obtain, so it has become a The main material of the current biomass energy research. [0003] At present, the production of ethanol from lignocellulose-containing raw materials usually adopts a bioc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P7/10C12R1/865
CPCC12N1/18C12P7/10C12R2001/865C12N1/185Y02E50/10
Inventor 佟毅何太波张子剑张媛李凡王康李义王小艳袁敬伟刘辉
Owner JILIN COFCO BIOCHEM