Platelet membrane self-assembly nanobubble, and preparation method and application thereof

A nano-bubble and platelet technology, which is applied in the field of biomedicine, can solve the problems of difficulty in the targeted ultrasound imaging diagnosis of small and micro lesions, and achieve the effects of good biological safety, good repeatability, and prolonged circulation time.

Active Publication Date: 2019-08-06
SOUTHEAST UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Purpose of the invention: Aiming at the problems existing in the prior art, the present invention provides a platelet membrane self-assembled nanobubble and its preparation method. The present invention can obtain nanobubbles with a nanometer size and retain the natural properties of the platelet membrane, and have a high biological phase Capacitance and vascular injury targeting, can be used for ultrasonic imaging diagnosis of vascular injury sites, and solve the difficult problem of targeted ultrasonic imaging diagnosis of small and micro lesions in the early stage of cardiovascular and cerebrovascular diseases

Method used

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  • Platelet membrane self-assembly nanobubble, and preparation method and application thereof
  • Platelet membrane self-assembly nanobubble, and preparation method and application thereof
  • Platelet membrane self-assembly nanobubble, and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0033] Set the concentration to 1 x 109 Fresh platelets per milliliter were separated by centrifugation at 500g for 10min, resuspended and washed with normal saline to remove plasma, and then placed in a -80°C refrigerator to freeze, frozen platelets were thawed at room temperature, and centrifuged at 4000g for 5min to separate, and The saline solution was resuspended and washed three times to separate the platelet membrane vesicles from the organelles to obtain a purified platelet membrane vesicle suspension. After the obtained platelet vesicle suspension was homogenized by 100W, 42KHz water bath ultrasound for 5min, it was broken by ultrasonic cavitation at 500W power for 30s, and sulfur hexafluoride gas was introduced during this process; to reduce ultrasonic cavitation Power to 100W for 90s, so that the platelet membrane fragments broken by ultrasonic cavitation are adsorbed on the nano-nucleus air-liquid interface formed by cavitation, and recombined to form a suspension o...

Embodiment 2

[0036] The platelet membrane extraction method in this example is the same as that in Example 1. The device for preparing nano-bubbles, the fixed volume of the vial is 3mL; the volume of the syringe of the volume-variable extrusion device can be changed in the range of 0-5mL; the volume of the connecting tube is 0.5mL, one end is connected to the volume-variable device, and the other end is inserted into the generating container for the vial Bottles containing water-purified platelet membrane vesicle suspensions.

[0037] 2 mL of platelet membrane vesicle suspension and 4.5 mL of 99.99% high-purity sulfur hexafluoride (SF 6 ) is accommodated in a nanobubble device to form a closed system. At this time, the equilibrium volume of the variable volume extruding device is 3mL; 6 Pressed into the water through the connecting pipe, at this time SF 6 The total volume is 1.5mL, and the system pressure is 0.3MPa; remove the external force, return the column sieve to the equilibrium po...

Embodiment 3

[0040] In this example, the platelet membrane extraction method is the same as in Example 1; the platelet membrane-coated nanobubble preparation method is the same as in Example 2, the only difference is that the sulfur hexafluoride gas used in Example 1 is replaced by helium. The microstructure of the prepared platelet membrane nanobubbles was characterized by a transmission electron microscope, which has a good bubble structure, and the assembly structure of the platelet membrane on the surface of the nanobubbles can be clearly observed.

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Abstract

The invention discloses a platelet membrane self-assembly nanobubble, and a preparation method and application thereof. The preparation process includes the following steps: (1) performing repeated freezing and thawing on platelets, and obtaining purified platelet membrane vesicle suspension through washing; and performing homogenization through a water bath ultrasonic effect; and (2) realizing the self-assembly and reorganization of platelet membrane fragments on a gas-liquid interface after the ultrasonic cavitation fragmentation or gas-liquid mixing repeated extrusion of the homogenized platelet membrane vesicle suspension so that the platelet membrane coated nanobubble can be constructed and formed. The preparation method of the nanobubble is simple; and the nanobubble with a nano sizeand reserving the natural properties of the platelet membranes has high biocompatibility and vascular injury targeting, and can be used for the ultrasound imaging diagnosis of vascular injury parts,and therefore, the problems of difficult targeted ultrasound imaging diagnosis of early small micro-lesions of cardio-cerebrovascular diseases can be solved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a platelet membrane self-assembled nano-bubble and its preparation method and application. Background technique [0002] Cardiovascular and cerebrovascular diseases are still the main cause of death in the world. Generally speaking, the prevalence and mortality of cardiovascular diseases in China are still on the rise. Residents are generally exposed to cardiovascular disease (CVD) risk factors, showing a trend of rapid growth and individual aggregation among younger age and low-income groups. It is estimated that the number of patients with cardiovascular diseases is 290 million, of which 13 million are stroke, 11 million are coronary heart disease, 5 million are cor pulmonale, 4.5 million are heart failure, 2.5 million are rheumatic heart disease, 2 million are congenital heart disease, and hypertension 270 million. Cardiovascular disease deaths accounted for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22
CPCA61K49/221A61K49/223
Inventor 杨芳李明熹顾宁
Owner SOUTHEAST UNIV
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