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A kind of filter sludge high-temperature composting bacterial agent lc and its application

A technology of high-temperature composting and filtering mud, which is applied in the field of recycling of agricultural and forestry waste, and can solve problems such as insufficient dissolved oxygen, loss of activity of bacterial strains, and limitation of high-temperature composting and stability of filtering mud.

Active Publication Date: 2022-03-15
广东省科学院南繁种业研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the process of composting filter mud, a large amount of water and cement will cause the filter mud to form agglomerates, insufficient dissolved oxygen, and it is difficult for microorganisms to enter and decompose, and the temperature increase in the filter mud composting process will cause many strains with fermentation and degradation functions to lose their activity— — is the main factor that limits the stability of high-temperature composting of filter sludge

Method used

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  • A kind of filter sludge high-temperature composting bacterial agent lc and its application
  • A kind of filter sludge high-temperature composting bacterial agent lc and its application
  • A kind of filter sludge high-temperature composting bacterial agent lc and its application

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Experimental program
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Effect test

Embodiment 1

[0044] Screening of high-temperature composting strains from filter mud:

[0045] The strain samples were derived from the mixed materials at the high temperature stage of the filter mud composting test at Maoyuan Sugar Industry Co., Ltd., Wengyuan County, Shaoguan City, Guangdong Province at a temperature of 50°C to 70°C. Weigh 10 g of bacterial strain samples, pass through a liquid enrichment medium with filter mud as the only nutrient source for one week (liquid enrichment medium is: 10 g of filter mud that has been dried to constant weight and crushed and passed through a 10-mesh sieve, added sterile water 90mL; Among them, the filter mud is from the filter mud formed by the sulfurous acid sugar production process of Maoyuan Sugar Industry Co., Ltd., Wengyuan County, Shaoguan City, Guangdong Province, the same below) to continue enrichment, after 3 days of enrichment, draw 10mL of enrichment solution to inoculate Continue to culture the second generation in the new liquid ...

Embodiment 2

[0053] Preparation of bacterial agent LC for high temperature composting of filter sludge

[0054] Inoculate the Bacillus licheniformis strain GBIF-3 on LB medium, shake and culture at 50°C and 170rpm for 1 to 2 days, centrifuge, remove the supernatant to get the precipitate, and reconstitute with 100mL of 0.9% sterilized normal saline Suspend to obtain the bacterial liquid, adjust the bacterial OD value of the bacterial liquid to 0.5; mix 85% of the bacterial liquid, 5% of bagasse, 5% of bagasse biochar and 5% of sugarcane molasses according to the mass fraction to obtain bacterial agent LC.

[0055] LB medium is: peptone 10g, yeast powder 5g, NaCl 10g, agar 20g, water 1000mL, pH natural.

[0056] The bagasse is the bagasse obtained by pressing the stalks of the sugarcane sugar factory, which is in a fiber state and is about 20 meshes; the bagasse biochar is prepared from bagasse after anaerobic combustion at 500°C for 2 hours, and is about 40 mesh. ~60 meshes; the sugarcane...

Embodiment 3

[0058] Determination of LC Enzyme Activity of High Temperature Composting Bacteria from Filter Sludge

[0059] High temperature treatment group: get the bacterium LC that embodiment 2 makes, according to weight ratio 5% (the weight ratio of bacterium LC and filter mud solid fermentation medium 5:100) inoculate to the 100g filter mud solid fermentation medium that is housed Put in a 250mL Erlenmeyer flask, place it in an incubator, and ferment for 30 days at a temperature of 50°C, take samples every day (destructive sampling), centrifuge the fermentation broth at 8000rpm / min, 4°C for 5min; filter to remove bacteria and solid impurities. Crude enzyme solution, assay lignocellulosic enzyme activity and protease activity.

[0060] Normal temperature treatment group: except that the temperature was adjusted from 50°C to 30°C, other operations were the same as the high temperature treatment group.

[0061] Blank control group 1: No bacterial agent LC was added, but an equal amount ...

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Abstract

The invention discloses a high-temperature composting bacteria agent LC for filter mud, which comprises Bacillus licheniformis (Bacillus licheniformis) GBIF-3, bagasse, bagasse biochar, and sugarcane molasses; the bacteria of GBIF-3 with a bacterial OD value of 0.5-1 The bacterial liquid is obtained by mixing 80%-90% by weight, 5%-10% by weight of bagasse, 1%-5% by weight of bagasse biochar and 1%-5% by weight of sugarcane molasses. The bacterial agent LC of the present invention can produce lignocellulosic hydrolase and protease, accelerate the decomposition and transformation of the filter mud substrate, and can promote the decomposing and decomposition of the filter mud in the composting process of the filter mud in sugarcane sugar factories under high temperature conditions. The invention also discloses the application of the filter sludge high-temperature composting bacterial agent LC in the solid fermentation of the filter sludge in sugarcane sugar factories and the application in high-temperature composting fermentation of filter sludge in sugarcane sugar factory to produce organic fertilizer.

Description

technical field [0001] The invention belongs to the technical field of agricultural and forestry waste recycling, and relates to a filter sludge high-temperature composting bacterial agent LC and an application thereof. Background technique [0002] my country is a big sugar consumption country, and more than 80% of the sugar comes from sugarcane raw materials. my country's sugarcane planting is mainly distributed in the three southern provinces of Guangxi, Yunnan, and Guangdong, and a small part is distributed in Hainan, Fujian, and Guizhou. In recent years, sugar imports have increased, but extensive sugarcane planting has laid a strong raw material foundation for ensuring self-sufficiency in sugar resources in my country. However, sugar cane will produce a lot of waste during the production, processing, pressing and sugar making process, such as bagasse leaves, filter mud, molasses and its fermentation alcohol residue. Will cause environmental pollution. Most of the su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/20C05F11/08C12R1/10
CPCC12N1/20C05F5/006C05F11/08C05F17/00Y02W30/40
Inventor 沈大春敖俊华陈迪文周文灵黄莹凌秋平卢颖林江永李奇伟
Owner 广东省科学院南繁种业研究所
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