Filter mud high-temperature composting inoculant LC and its application

A technology of high-temperature composting and filtering mud, which is applied in the field of recycling of agricultural and forestry wastes, can solve the problems of limiting the high-temperature composting of filter mud to decompose and stabilize, microorganisms are difficult to enter and decompose, and bacterial strains lose their activity, so as to achieve sustainable development, promote recycling, The effect of promoting decomposition

Active Publication Date: 2019-08-06
广东省科学院南繁种业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the process of composting filter mud, a large amount of water and cement will cause the filter mud to form agglomerates, insufficient dissolved oxygen, and it is difficult for microorganisms to enter and decompose, and the t

Method used

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  • Filter mud high-temperature composting inoculant LC and its application
  • Filter mud high-temperature composting inoculant LC and its application
  • Filter mud high-temperature composting inoculant LC and its application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0043] Example 1

[0044] Screening of strains for high-temperature composting of filter sludge:

[0045] The strain samples were derived from the mixture of 50℃~70℃ in the high temperature stage of the filter mud composting test in Maoyuan Sugar Co., Ltd. of Wengyuan County, Shaoguan City, Guangdong Province. Weigh 10 g of the strain sample and pass it through a week of liquid enrichment medium with filter mud as the only nutrient source (liquid enrichment medium is: 10 g of filter mud dried to constant weight, crushed and passed through a 10-mesh sieve, added sterile water 90mL; among them, the filter mud is derived from the filter mud formed by the sulfite process sugar production process of Maoyuan Sugar Co., Ltd., Wengyuan County, Shaoguan City, Guangdong Province, the same below) continue to enrich, 3 days after the enrichment, suck 10mL of enriched solution for inoculation Continue to culture the second generation in the new liquid enriched medium, and then after the third ...

Example Embodiment

[0052] Example 2

[0053] Preparation of bacterial agent LC for high-temperature composting of filter sludge

[0054] The Bacillus licheniformis strain GBIF-3 was inoculated on LB medium, cultured with shaking at a temperature of 50°C and a rotating speed of 170 rpm for 1 to 2 days, centrifuged, removed the supernatant to take the precipitate, and then reconstituted with 100 mL of 0.9% sterile physiological saline Suspended to obtain a bacterial liquid, adjust the bacterial OD of the bacterial liquid to 0.5; mix 85% of the bacterial liquid, 5% of bagasse, 5% of bagasse biochar and 5% of sugar cane molasses according to the mass fraction to obtain the bacterial agent LC.

[0055] The LB medium is: peptone 10g, yeast powder 5g, NaCl 10g, agar 20g, water 1000mL, and the pH is natural.

[0056] The bagasse is the bagasse of the sugarcane sugar plant after the squeezing of the cane, in a fibrous state, about 20 mesh; the bagasse biochar bagasse is prepared after anaerobic combustion at 500...

Example Embodiment

[0057] Example 3

[0058] Determination of LC enzyme activity of filter sludge high-temperature composting bacterial agent

[0059] High-temperature treatment group: Take the bacterial agent LC prepared in Example 2 and inoculate it in a weight ratio of 5% (weight ratio of bacterial agent LC and filter mud solid fermentation medium 5:100) to 100g filter mud solid fermentation medium In a 250mL Erlenmeyer flask, placed in an incubator, solid fermentation at a temperature of 50 ℃ for 30 days, sampling every day (destructive sampling), fermentation broth 8000 rpm / min, 4 ℃ centrifugation for 5 minutes; filtered to remove bacteria and solid impurities Crude enzyme solution, determination of lignocellulosic enzyme activity and protease activity.

[0060] Normal temperature treatment group: Except for adjusting the temperature from 50°C to 30°C, other operations are the same as the high temperature treatment group.

[0061] Blank control group 1: Do not add bacterial agent LC, but add the s...

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Abstract

The invention discloses a filter mud high-temperature composting inoculant LC, which comprises Bacillus licheniformis GBIF-3, bagasse, bagasse biochar and cane molasses; The filter mud high-temperature composting inoculant LC is prepared by mixing the following components in parts by weight: 80%-90% of a bacterial solution of GBIF-3 with a bacterial OD value of 0.5-1, 5%-10% of bagasse, 1%-5% of bagasse biochar, and 1% to 5% cane molasses. The inoculant LC of the invention can produce lignocellulosic hydrolase and protease, accelerates the decomposition and conversion of a filtrate substrate,and can promote the decomposition of the sludge in a sludge composting process of a sugar cane plant under high temperature conditions. The invention also discloses an application of the filter mud high-temperature composting inoculant LC in the solid fermentation of the sludge in the sugar cane plant and the application of an organic fertilizer in the high temperature compost fermentation of thesugar cane plant.

Description

technical field [0001] The invention belongs to the technical field of agricultural and forestry waste recycling, and relates to a filter sludge high-temperature composting bacterial agent LC and an application thereof. Background technique [0002] my country is a big sugar consumption country, and more than 80% of the sugar comes from sugarcane raw materials. my country's sugarcane planting is mainly distributed in the three southern provinces of Guangxi, Yunnan, and Guangdong, and a small part is distributed in Hainan, Fujian, and Guizhou. In recent years, sugar imports have increased, but extensive sugarcane planting has laid a strong raw material foundation for ensuring self-sufficiency in sugar resources in my country. However, sugar cane will produce a lot of waste during the production, processing, pressing and sugar making process, such as bagasse leaves, filter mud, molasses and its fermentation alcohol residue. Will cause environmental pollution. Most of the su...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F15/00C05F17/00C05F11/08C12R1/10
CPCC12N1/20C05F5/006C05F11/08C05F17/00Y02W30/40
Inventor 沈大春敖俊华陈迪文周文灵黄莹凌秋平卢颖林江永李奇伟
Owner 广东省科学院南繁种业研究所
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