Kit and method for rapidly detecting endocrine disrupting activity of chemical substance based on magnetic microspheres

Abstract

The invention provides a kit and method for rapidly detecting the endocrine disrupting activity of a chemical substance based on magnetic microspheres. The kit comprises a microsphere-GST/6*HIS-NR LBDprotein polymer solution, an SRC-BAP protein solution, a BSA Solution, a reaction solution, a washing solution, a color developing solution and a coloring stop solution. According to the invention, the activated microsphere-protein polymer solution, the SRC-BAP protein solution, the BSA solution and a to-be-detected substance are mixed and incubation is performed; after cleaning, the color developing solution is added and color development is carried out and then is stopped; and a light absorption value is measured by a common optical microplate reader; the light absorption value is processedby software and EC50 or IC50 is calculated, so that the receptor activity of a chemical substance is quickly determined. With the provided detecting method, the experimental period can be shortened,the purity and activity of the receptor can be enhanced, and the detection sensitivity can be improved, so that an objective of detecting and screening the endocrine disrupting receptor activity in the chemical product sensitively, efficiently, and rapidly is achieved.

Description

technical field [0001] The invention relates to the technical field of detection of the disrupting effect of chemicals on the human endocrine system, in particular to a kit and method for rapidly detecting the endocrine disrupting activity of chemical substances based on magnetic microspheres. Background technique [0002] With the rapid development of industrialization and urbanization, tens of thousands of chemicals are discharged into the natural ecological environment every year through sewage, solid waste, and the atmosphere. Among them, bisphenols, alkylphenols, organic phosphates, Environmental endocrine disruptors such as parabens can interact with nuclear receptors that are closely related to human health, such as estrogen receptors, estrogen-related receptors, androgen receptors, and thyroid hormone receptors. The normal physiological functions in the body lead to damage to the body and even induce cancerous changes such as tumor masses. Environmental endocrine di...

AI Technical Summary

Problems solved by technology

Existing detection methods mainly include in vitro microwell plate coating protein-enzyme color reaction, yeast two-hybrid, mammalian dual-luciferase reporter system, animal live experiments, etc. One or more of the shortcomings of expensive equipment, poor experimental repeatability, and low sensitivity, without taking into account the actual needs of high sensitivity and high efficiency, it is difficult to meet the requirements of large quantities of rapid detection of chemical endocrine disrupting receptor activity

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