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Microbial inoculum for preparing caged chicken fermentation mattress material and preparation method of inoculum

A technology of composite microbial agent and microbial composition, which is applied in the field of microbial engineering, can solve the problems of chicken manure with little decomposition effect, no fermentation function, and no obvious heat production, so as to improve the humus rate of litter and improve the microbial efficiency. Diversity, ammonia gas reduction effect

Active Publication Date: 2019-08-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Traditional chicken litter is relatively thin, generally about 10cm. The change of chicken manure in the litter is mainly due to the process of corruption. It has no fermentation function and has little effect on the decomposition of chicken manure. The litter mainly absorbs the moisture and odor of chicken manure. The role of air, no obvious heat production, the litter is relatively wet, and the odor in the litter and the chicken house is serious

Method used

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  • Microbial inoculum for preparing caged chicken fermentation mattress material and preparation method of inoculum
  • Microbial inoculum for preparing caged chicken fermentation mattress material and preparation method of inoculum
  • Microbial inoculum for preparing caged chicken fermentation mattress material and preparation method of inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Separation of Bacillus megaterium and Lactobacillus plantarum

[0037] Isolation of Bacillus megaterium: Take 10g of decomposed chicken manure and connect it to 200mL enriched medium (2g ammonium sulfate, 2.17g sodium succinate, 0.25g potassium dihydrogen phosphate, 0.125g magnesium sulfate, 0.125g NaC1, 1000mL water) In, every 3 days, transfer to a new enrichment medium with an inoculum of 3%, continue enrichment culture for 30 days, dilute and spread on an ammonium sulfate plate, select fast-growing large colonies, and repeat separation and purification twice . The obtained strains were inoculated into the enriched medium for shaking culture, and at the same time, Gliss reagent and diphenylamine reagent were used to track and detect the production of nitrite and nitrate. 28 strains were obtained by preliminary screening, and then the strains were multiplexed into chicken manure In the extract (preparation of chicken manure extract: mix chicken manure and tap ...

Embodiment 2

[0039] Example 2 Preparation of composite microbial agent

[0040] The configuration of the compound microbial agent goes through the following three steps from the inclined surface bacteria to the amplification of the finished product.

[0041] 1. Primary expansion: After each purified and cultivated strains (Bacillus amyloliquefaciens, Bacillus megaterium, Trichoderma longiflorum, Lactobacillus plantarum) are taken out of the preserved slope, they are respectively inoculated into the four prepared seeds Culture medium separately. Each 1L bottle contains 200ml medium. Lactobacillus plantarum is cultured in a constant temperature incubator at 37°C for 48h. Bacillus amyloliquefaciens and Bacillus megaterium are cultured on a shaker at 35°C at 150r / min for 48h. , Trichoderma longibrachiatum was cultured with shaking at 30°C at 120r / min for 96h on a shaker.

[0042] In the first-level expansion culture, the slant medium and seed medium of various bacteria are well-known basic medium. ...

Embodiment 3

[0046] Example 3: The effect test of the compound microbial starter of the present invention on the caged chicken fermentation bed

[0047] From October 2017 to December 2017, an application experiment of the product of the present invention on the bed surface substrate was carried out in a chicken farm. The fermentation bed of caged chicken is about 40cm with fungus chaff, and the surface is about 10cm of sawdust / rice husk. The weight ratio of sawdust to rice husk is 1:1. Run and stir with a rake machine to mix the litter. The thickness of the litter is 50cm. The test group uses 50 grams of fermentation bed bacteria per square meter of fermentation bed. The method of sprinkling is to mix the fermentation bed bacteria and bran evenly, then sprinkle them on the surface of the fermentation bed, and run and stir with the rake machine; The weight ratio of bran is: 50g of fermentation bed inoculum and 1kg of bran. The control group only sprinkled 1kg of bran per square meter.

[0048]...

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Abstract

The invention discloses a microbial inoculum for preparing a caged chicken fermentation mattress material and a preparation method of the inoculum. The composite microbial inoculum is prepared by inoculating a composition consisting of a bacillus amyloliquefaciens fermentation liquid, a bacillus megaterium fermentation liquid, a trichoderma longibrachiatum fermentation liquid and a lactobacillus plantarum fermentation liquid into a solid culture medium, uniformly mixing, culturing for 72-96 hours under a culture condition of 30-35 DEG C, and drying. The invention discloses an application of the composite microbial inoculum in preparing the caged chicken fermentation mattress material. The composite microbial inoculum is prepared by selecting the best proportion of four excellent strains, the functional strains cooperate in the feeding and management process of the caged chicken fermentation bed surface, the functions of the organic matter transforming bacteria, the ammonia fixing deodorizing bacteria and the bacteria inhibiting bacteria are complementary, the temperature rise fermentation of the fermentation bed is rapidly started, the rapid removal of moisture in feces is realized, the microbial diversity of the fermentation bed is improved, the growth of pathogenic spoilage bacteria is inhibited, the in-situ degradation of chicken manure is promoted, and the humification rateof the mattress material is improved.

Description

Technical field [0001] The invention discloses a microbial inoculum for preparing fermented mattress material for caged chickens and a preparation method thereof, and belongs to the technical field of microbial engineering. Background technique [0002] At present, large-scale chicken farms adopt the cage-raising mode. While the cage-raising method improves the production level and labor efficiency of the chicken farm, there are also problems such as concentrated discharge of chicken manure and deterioration of the chicken farm’s own environment. Manure pollution has become a constraint on the cage. An important factor in chicken breeding. The manure and urine treatment technology of caged chickens is mostly processed by composting fermentation, biogas fermentation, aerobic fermentation and drying technology, etc. Composting fermentation treatment of manure occupies land, which causes great air pollution to the environment, and biogas fermentation has seasonal limitations. The c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01K31/04C12R1/07C12R1/11C12R1/885C12R1/25
CPCC12N1/20C12N1/14A01K31/04
Inventor 宦海琳杨智青丁海荣顾洪如丁成龙时凯
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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