Enterobacter z1 and Klebsiella z2 complex bacterial agent to remove high nitrogen pollution wastewater and its application
A Klebsiella and Enterobacter technology, applied in water pollutants, biological water/sewage treatment, water/sludge/sewage treatment, etc. The effect of removing and reducing COD
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Embodiment 1
[0024] Example 1: Isolation of Enterobacter Z1 and Klebsiella Z2 from underground sediments in Shashi District, Jingzhou City, Hubei Province
[0025] (1) Sample collection: In May 2010, the sediment wet soil polluted by Cr(VI) rich in nitrate was collected in Shashi City, Jingzhou City, Hubei Province.
[0026] (2) Sample enrichment: Accurately weigh 100g of soil sample into a 250mL sterilized Erlenmeyer flask, add 5mL of 500mM ammonium chloride NH 4 Cl(NH 4 + ), stirred gently and placed in an incubator at 28°C for one week, paying attention to supplementing ammonia nitrogen and sterile water to ensure that the samples were not dry.
[0027] (3) Strain isolation: Accurately weigh NH 4 + 10g of the enriched soil sample was placed in a conical flask filled with 90mL of sterile normal saline, placed in a shaker at 28°C for one hour, taken out and left to stand for two hours, and then gradually diluted to 10 mL with 9mL of sterile normal saline. -3 、10 -4 、10 -5 , respect...
Embodiment 2
[0040] Example 2: Removal Curves of Enterobacter Z1 and Klebsiella Z2 Adding Nitrogen in BM Medium
[0041] Pick single clones of Enterobacter Z1 and Klebsiella Z2 strains and inoculate them into 100 mL of BM liquid medium, and culture them in a shaker at 28°C until OD 600 It is about 0.5, it is used as seed liquid, inoculates in fresh 100mL BM liquid culture medium (initial OD 600 value4 + , NO 2 - , NO 3 - . It was placed in a shaker at 28°C for shaking culture. Samples were taken every 6 hours. After centrifugation at 12000rpm for 2min, the content changes of various forms of nitrogen in the supernatant were measured. Determination steps see embodiment 1.
[0042] It can be seen from Table 2 that both of the above two strains can grow in BM medium with various inorganic nitrogen ions as the sole nitrogen source and can effectively remove various inorganic nitrogen ions in the reaction system. In the reaction system where nitrate nitrogen is added, nitrogen is main...
Embodiment 3
[0046] Embodiment 3: Enterobacter Z1 and Klebsiella Z2 are to the removal curve of various inorganic nitrogen ions and the effect of reducing the chemical oxygen demand (COD) in aquaculture wastewater in natural high-concentration N-polluted water bodies
[0047] The present invention uses two kinds of high-concentration N to pollute the water body, and the properties of the water body are shown in Table 3
[0048] The basic properties of two kinds of waste water that the present invention adopts of table 3
[0049]
[0050] The specific steps are as follows: prepare a 250mL triangular flask, fill it with 100mL natural water body (no need to sterilize), add cultured bacteria to each bottle, and divide it into three test groups: inoculate Enterobacteriaceae Z1 (seed solution OD 600 0.5 or so, 1% inoculum size), inoculate Klebsiella Z2 (seed solution OD 600 0.5 or so, 1% inoculum size), inoculate Enterobacteriaceae Z1 and Klebsiella Z2 (seed solution OD 600 is about 0.5, 0....
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