Method for detecting and identifying cysteine and homocysteine based on liquid crystal sensing platform
A homocysteine and sensing platform technology, which is applied in the field of metal ion coordination detection of amino acids, can solve the problems of complex operation steps, time-consuming sample preparation, and obstacles to practical application, and achieve low reagent consumption, short detection time, and solution The effect of detection method complexity
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Embodiment 1
[0039] Embodiment 1 Construction of liquid crystal sensing platform
[0040] The construction method of the liquid crystal sensing platform is as follows:
[0041] i. Using the glass slide as the substrate of the liquid crystal sensing platform, soak the glass slide in the "piranha" washing solution for 30 minutes at 75°C, and then wash with ultrapure water, absolute ethanol, and methanol for 3 times respectively. Blow dry with nitrogen, and dry at 120°C for 12 hours; soak the dried glass slide in n-heptane solution containing 5mM octadecyltrichlorosilane for 30min, rinse with dichloromethane, and dry with nitrogen to obtain silane Base reagent-treated glass slides;
[0042] ii. Place a copper grid on the glass slide prepared in step i, and inject thermotropic liquid crystal 4-cyano-4'-pentylbiphenyl into the copper grid to construct a liquid crystal sensing platform.
[0043] Among them, the "piranha" lotion is prepared by using concentrated sulfuric acid with a mass concen...
Embodiment 2
[0045] Embodiment 2 detects the determination of the used copper perchlorate solution concentration of cysteine and homocysteine
[0046] Dissolve copper perchlorate in ultrapure water to prepare aqueous solutions with concentrations of 100mM, 80mM, 50mM, 20mM, 10mM, 5mM, and 2mM, and add the above-mentioned copper perchlorate solutions of different concentrations to the liquid crystal sensing platform. On, the drop volume is 50μL, observed under a polarizing microscope, the results are as follows figure 2 As shown, bright optical images were observed under a polarizing microscope. Subsequently, 1.0 mg / mL cysteine solution and homocysteine solution were added to the liquid crystal sensing platform containing different concentrations of copper perchlorate solution, and the drop volume was 50 μL, and observed using a polarizing microscope (such as image 3 ), it was found that when the concentration of copper perchlorate solution>10mM, after adding two mercapto amino aci...
Embodiment 3
[0047] Example 3 Detection and identification of cysteine and homocysteine based on liquid crystal sensing platform
[0048] The standard cysteine and homocysteine were ultrasonically dissolved in ultrapure water, the time for ultrasonic dissolution was about 30min, and the concentration of cysteine was prepared to be 1.0mg / mL, 0.1mg / mL, 0.01mg / mL, 0.005mg / mL, 0.001mg / mL, the concentration of homocysteine was prepared to be 1.0mg / mL, 0.1mg / mL, 0.05mg / mL, 0.01mg / mL, 0.001mg / mL; Concentrated cysteine solution and homocysteine solution were added dropwise to the liquid crystal sensing platform containing 10mM copper perchlorate solution, copper perchlorate solution, cysteine solution and homocysteine solution The dropping volume of each was 50 μL, and after a period of time, the optical image of the liquid crystal was observed under a polarizing microscope, and the results were as follows: Figure 4 As shown, adding a concentration of cysteine ≥ 0.01mg / mL,...
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