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The method of artificial oxytocin fertilization of yellow croaker

A technology of artificial induced labor and croaker, applied in the biological field, can solve problems such as unfavorable production, asynchronous development, asynchronous spawning, etc.

Active Publication Date: 2021-09-14
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the artificial breeding of yellow croaker relies on the natural maturation of the parents to achieve spawning. Due to the difference in the maturity of the parents of croaker, the gonad development of male and female fish is not synchronized, and the gonads of male fish mature earlier than female fish, and the gonad development of female fish is generally slow. There are obvious individual differences, which can easily cause asynchronous spawning, and artificial control of spawning time and spawning amount cannot be realized, which is not conducive to production, nor can it meet the requirements of fish genetic breeding for directional mating

Method used

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  • The method of artificial oxytocin fertilization of yellow croaker
  • The method of artificial oxytocin fertilization of yellow croaker
  • The method of artificial oxytocin fertilization of yellow croaker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The method for preparing a composition with stability, comprising, a, preparing a buffer solution and adjusting its pH; b, adding oxytocin having an oxytocin function to the buffer solution; and thereafter, c, adding to the dispersion obtained in step b Add at least one non-ionic cellulose ether; the buffer solution is alkaline; the oxytocin includes N-acetyl-L-cysteine-coated microspheres. The stability of the biological substance in the composition produced by the method is improved, in particular the biological activity and therapeutic effect of the presented composition is improved by avoiding excessive degradation or polymerization, or other structural changes of the oxytocin substance, The biological activity or therapeutic activity of oxytocin is improved and prolonged, and it is easier for the user to handle. Due to the improved biological activity, the composition does not always need to be stored in a cold environment (-15 ~ -5°C) It can be more conveniently s...

Embodiment 2

[0045] The artificial oxytocin fertilization method of yellow croaker, the specific measures are as follows:

[0046] 1) After mixing N-acetyl-L-cysteine ​​and gelatin, add 6 times the amount of water, let it stand for swelling for 2.5 hours, heat to 70°C and stir until dissolved to make an inner water phase, the above-mentioned inner water phase The weight proportions of N-acetyl-L-cysteine ​​and gelatin are respectively 11% and 19%, and the above-mentioned internal water phase also contains 0.15% uric acid and 0.05% gluconic acid-δ - Lactone;

[0047] 2) Dissolve ethyl cellulose in dichloromethane to make an oil phase with a weight ratio of 13%, then add the inner water phase into the oil phase at a weight ratio of 1:22, and ultrasonically Crush for 5 minutes to form colostrum;

[0048] 3) Take polyvinyl alcohol and add water and heat it to 70°C and stir until it dissolves to make an external water phase with a weight concentration of 1.5%, and then disperse the colostrum ...

Embodiment 3

[0056] The differences between this embodiment and embodiment 2 are:

[0057] The composition in step 6) is injected into the broodstock in the form of injection administration once a day on the 58th-60th day of cultivation, and the dosage is 50 mg / kg body weight each time;

[0058] Other steps are consistent with embodiment 2, cultivate yellow croaker fry.

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Abstract

The invention provides an artificial induced fertilization method for yellow croaker, which belongs to the field of biotechnology, and includes feeding the parent croaker with the stable composition prepared in the present invention during the cultivation period of the parent of croaker croaker, and carrying out nutritional strengthening , after the parents are sexually mature, carry out artificial egg collection to complete insemination, obtain fertilized eggs, hatch the fertilized eggs, and cultivate fry. The artificial oxytocin fertilization method of the present invention can adjust the final synchronous maturation of eggs and sperm to avoid the phenomenon of asynchronous and unsmooth spawning and ejaculation, avoid postpartum broodstock death loss, the female fish has a large amount of ovulation, and the male fish has high semen quality, which can improve the hatching rate and Effective hatching capacity to increase the output of fry; the composition is stable and easy to store, can increase the accumulation of fat, protein and total sugar in the testis and ovary, make the ovary and testis of the parents mature synchronously, and increase the ovulation capacity of the parent female fish.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an artificial induced fertilization method for yellow croaker. Background technique [0002] Yellow croaker (Nibea albiflora), commonly known as yellow croaker, belongs to Perciformes, Chrysostidae, and genus Yellow croaker. Its body shape is similar to yellow croaker. It is a fish in the middle and lower waters of the sea, mainly inhabiting sandy mud bottoms It is an important multiplication and breeding species in the southeast coast of my country. Its meat is delicious, nutritious, and has high nutritional and medicinal value. The market demand on the rise. It is worth mentioning that the yellow croaker has an insect-resistant peptide gene that the large yellow croaker does not have, and has special immunity to stimulating Cryptocaryonia, and can be used as the preferred alternative breeding species in areas with high incidence of white spot disease. [0003] Due to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 苗增良
Owner ZHEJIANG OCEAN UNIV
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