Tetraphenylethylene derivative fluorescent probe and preparation method thereof
A fluorescent probe, tetraphenylethylene technology, which is applied in the field of aggregation-induced luminescence fluorescent materials, can solve the problems of difficult preparation conditions of tetraphenylethylene derivatives, insoluble in water, etc., and achieves good water solubility, simple synthesis and wide application. Effect
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[0038] Example 1: Synthesis of tetrastyrene derivative fluorescent probe
[0039] 1. Compound M 1 Synthesis
[0040] Take a clean 500mL three-necked flask, vacuum it several times, add 4-methylbenzophenone (19.6g, 0.1mol) and zinc powder (20g, 0.308mol) to the three-necked flask under the protection of argon, and then Add 300 mL of tetrahydrofuran to the flask, turn on the magnetic stirrer and stir, seal a flask mouth with plastic wrap, and tie it tightly with a rubber band. Slowly pour liquid nitrogen into the ethanol bath to cool, when it is cooled to -78℃, measure 20mL of titanium tetrachloride 4 , Inhaled into the syringe, pierce the plastic wrap and slowly and evenly add it into the flask, while continuing to pour liquid nitrogen to maintain the reaction temperature. After the addition is completed, a balloon is placed on the spherical condenser, and the reaction is stirred for 1.5 hours, and the balloon is deflated in time. After stirring and reacting for 1.5 hours, the oil...
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[0054] Example 2: M 3 As a drug carrier to encapsulate adriamycin
[0055] First dissolve doxorubicin (DOX) in M 3 (246mg, 0.5mmol) in ethanol solution (25mL) (3.3×10 -3 M), add an aqueous solution (5mL) of zinc nitrate hexahydrate (150mg, 0.5mmol) at room temperature and stir vigorously at room temperature to obtain DOX / Zn(M 3 )ball. The coated metal-organic system was purified by centrifugation and washed with ethanol for several times to obtain a red solid. The coated particles are re-dispersed in ethanol or phosphate buffer to obtain the corresponding colloid.
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[0056] Example 3: Ultraviolet absorption spectrum measurement
[0057] (1) Target product M 3 Molecular UV absorption spectrometry
[0058] Pipette 2mL M 3 The molecule to be tested (0.01mmol / L) was transferred to a 4mL cuvette. The preset ultraviolet absorption wavelength range is determined to be between 200nm and 600nm, and a blank test is done first to deduct the blank interference of the solvent used, and finally the ultraviolet absorption spectrum is measured. get figure 1 , Which is M 3 The ultraviolet absorption spectrum of the molecule.
[0059] (2) Target product M 4 Molecular UV absorption spectrometry
[0060] Pipette 2mL M 3 The molecule to be tested (0.05mg / mL) was transferred to a 4mL cuvette. The preset ultraviolet absorption wavelength range is determined to be between 200nm and 600nm, and a blank test is done first to deduct the blank interference of the solvent used, and finally the ultraviolet absorption spectrum is measured. get figure 2 , Which is M 4 The ult...
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