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A kind of biological adhesive based on biological engineering protein and preparation method thereof

A bioadhesive, bioengineering technology, applied in the preparation of protein adhesives, animal glue or gelatin, adhesive types, etc., can solve the lack of non-invasive technology, fixation and bone atrophy, poor bone healing, etc. problem, to achieve the effect of no special equipment, low cost, good biocompatibility and degradability

Active Publication Date: 2020-07-07
BEIJING COMPONT MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the modulus mismatch between hard bone material and bone tissue leads to localized stresses for fixation and bone atrophy
Metal grafts are ancillary vehicles that do not chemically react with bone and are often affected by aseptic relaxation, resulting in poor bone healing
Despite these shortcomings, suturing remains the gold standard for tissue reconstruction due to the lack of non-invasive techniques capable of surpassing them
On the other hand, our daily life experience shows that conventional adhesives exhibit strong adhesion in a dry environment, but are easily destroyed in the presence of water

Method used

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  • A kind of biological adhesive based on biological engineering protein and preparation method thereof
  • A kind of biological adhesive based on biological engineering protein and preparation method thereof
  • A kind of biological adhesive based on biological engineering protein and preparation method thereof

Examples

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preparation example Construction

[0033] The present invention provides a kind of preparation method of bioadhesive based on bioengineering protein, comprises the following steps:

[0034] A) respectively dissolving the positively charged elastin-like protein and the alkyl-type anionic surfactant in ultrapure water to obtain an elastin-like protein solution and a surfactant solution;

[0035] The alkyl anionic surfactant comprises sodium dodecylbenzenesulfonate and / or sodium dodecylsulfonate;

[0036] B) adding the surfactant solution dropwise into the elastin-like protein solution, and mixing uniformly to obtain a binder solution;

[0037] C) centrifuging the adhesive solution and then freeze-drying to obtain a bioadhesive.

[0038] In the present invention, the type, source and amount of the positively charged elastin-like and alkyl anionic surfactants are the same as those of the above-mentioned positively charged elastin-like and alkyl anionic surfactants. The types, sources and dosages are the same and ...

Embodiment 1

[0046] Transform the positively charged elastin-like ELP vector plasmid into Escherichia coli expression strain BL21DE3, pick a single clone colony, and culture overnight with LB culture medium; add the overnight activated expression seed liquid to TB medium until the bacterial liquid reaches OD600 When the value is 0.8, the inducer isopropyl-β-D-thiogalactopyranoside is added and the temperature is lowered to 30 degrees for overexpression. Induce for 12 hours to collect the bacteria, resuspend with lysis buffer, add protease inhibitors, DNase, lysozyme, break the bacteria with a high-pressure crusher, centrifuge at a speed of 10,000 rpm or more, collect the supernatant, and purify by HPLC to finally obtain Charged elastin-like ELP.

[0047] Take 11 mg of ELP protein and 7.5 mg of sodium dodecylbenzenesulfonate, and add them into 50 μL of ultrapure water respectively. The surfactant aqueous solution was added dropwise to the ELP protein aqueous solution, shaken and centrifuge...

Embodiment 2

[0051] Take 8.7 mg of the ELP protein prepared in Example 1 and 6 mg of sodium dodecylbenzenesulfonate, and add them into 50 μL of ultrapure water respectively. The surfactant aqueous solution was added dropwise to the ELP protein aqueous solution, shaken and centrifuged for 10 minutes, and the precipitate layer was left to freeze-dry for 20 minutes to obtain the ELP adhesive.

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Abstract

The invention provides a biological adhesive based on bioengineering protein and a preparation method thereof, which is composed of positively charged elastin-like proteins and alkyl anionic surfactants; the alkyl anionic surfactants include dodecane Sodium phenyl sulfonate and / or sodium dodecyl sulfonate. The present invention synthesizes the elastin-like molecule ELP containing a large number of positive charges through recombinant protein technology, and then combines it with an anionic surfactant through the electrostatic force on the side chain of the polypeptide backbone to prepare a biomedical adhesive with high adhesive strength . The obtained product can exhibit excellent adhesive properties under different conditions (dry sample, wet sample and animal skin), and can be used for hemostasis and wound repair; meanwhile, it has good biocompatibility and degradability, and the preparation process is simple and does not require Special equipment, low cost, and environmental protection can be developed into a new generation of biomedical glue.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a high-strength bioadhesive based on bioengineering proteins and a preparation method thereof. Background technique [0002] An adhesive is a material used to bond two or more surfaces together under defined conditions. It plays an important role in the fields of high technology and biomedicine. Likewise, in nature, adhesion across multiple length scales is essential for survival. Inspired by nature, researchers have synthesized various biomimetic adhesives through the study of various adhesion mechanisms of biological organisms. However, these adhesives usually have a long synthesis cycle and are expensive, and some adhesives need to be glued by ultraviolet radiation, which greatly limits the application of existing adhesives in the fields of medicine and industry. Worldwide a large number of surgical procedures are performed every year, in a recent study over 300 millio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09J189/00C09J11/06
CPCC09J11/06C09J189/00C08K5/42
Inventor 刘凯马超孙静张洪杰
Owner BEIJING COMPONT MEDICAL DEVICES