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Bifidobacterium lactis nbk-w13 and its application

A technology of nbk-w13 and bifidobacterium lactis, which is applied in the direction of bifidobacteria, lactobacillus, application, etc., can solve the problems of not fully exerting the efficacy of the product, rapid decay of the number of viable bacteria, poor stability of bifidobacterium lactis, etc. problems, to achieve the effects of non-specific immune function improvement, enhanced immunity, strong acid and bile salt resistance

Active Publication Date: 2021-03-19
诺佰克(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common Bifidobacterium lactis products on the market are ubiquitous. When the number of viable bacteria is insufficient, the efficacy of the product cannot be fully exerted, and Bifidobacterium lactis has defects such as poor stability and rapid decay of the number of viable bacteria. Therefore, it is urgently needed Development of new strains of Bifidobacterium lactis with multiple functions

Method used

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  • Bifidobacterium lactis nbk-w13 and its application
  • Bifidobacterium lactis nbk-w13 and its application
  • Bifidobacterium lactis nbk-w13 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Screening and Identification of Bifidobacterium lactis nbk-W13

[0052] The feces of healthy infants were collected, ground, diluted with sterile normal saline to a suitable gradient, drawn on the modified MRS agar plate using the four-section line method, and anaerobically cultured in an incubator at 30-42°C for 24-48h. A single colony was selected, examined under a microscope, streaked, and further purified to obtain strain nbk-W13.

[0053] Microbiological characteristics and physiological and biochemical characteristics of strain nbk-W13:

[0054] Cell morphology

[0055] experimental project result Cell morphology Rod s Positive Oxidase Negative Catalase Negative

[0056] Physical and chemical experiment results

[0057]

[0058] The 16S rDNA sequence of strain nbk-W13 is shown in SEQ ID NO:1. According to the identification results of microbiology and molecular biology, the strain nbk-W13 was identified ...

Embodiment 2

[0059] Example 2 Preparation of Bifidobacterium lactis nbk-W13 freeze-dried powder

[0060] The preparation method of bifidobacterium lactis nbk-W13 freeze-dried powder comprises the following steps:

[0061] (1) inoculating Bifidobacterium lactis nbk-W13 into the seed medium for activation to obtain a primary seed liquid;

[0062] (2) inoculating the primary seed liquid into the seed medium for anaerobic cultivation for 8-24 hours to obtain the secondary seed liquid;

[0063] (3) inserting the secondary seed liquid into the fermentation medium to carry out high-density fermentation, centrifuging to get the precipitate, and obtaining the bacteria sludge;

[0064] (4) emulsifying embedding after mixing the bacteria slime and the freeze-drying protective agent to obtain an emulsion;

[0065] (5) The obtained emulsion was freeze-dried and pulverized to obtain the freeze-dried powder of Bifidobacterium lactis nbk-W13.

[0066] In terms of mass percentage, the formula of the see...

Embodiment 3

[0070] Example 3 Preparation of Bifidobacterium lactis nbk-W13 inactivated bacteria powder

[0071] In terms of mass percentage, the formula of the seed medium is: 1.5% lactose, 1.8% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.4% ammonium citrate, 0.02% magnesium sulfate, 0.06% manganese sulfate, 0.06 % L-cysteine ​​hydrochloride, 0.1% Tween 80 and the rest of water, pH 6.8;

[0072] In terms of mass percentage, the formula of the fermentation medium is: 2% lactose, 1.3% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.2% ammonium citrate, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.06 % L-cysteine ​​hydrochloride, 0.1% Tween 80 and balance water, pH 6.4.

[0073] The specific preparation method of bifidobacterium lactis nbk-W13 inactivated bacteria powder is as follows:

[0074] 1) inoculating Bifidobacterium lactis into a test tube containing a seed medium for activation (37°C, 20h) to obtain a first-grade seed liquid;

[0075] 2) Tr...

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Abstract

The invention provides bifidobacterium lactis nbk-W13 and application thereof. The bifidobacterium lactis nbk-W13 provided by the present invention has strong acid-resistant and bile-salt-resistant characteristics, and significantly enhances immunity. To study its role in the intestinal mucosal immunity of mice, it was found that when the immunocompromised model mice and normal mice were orally administered the Bifidobacterium lactis for 14 days, their specific cellular immune function, specific humoral immune function, nonspecific immune function Functions have been effectively improved. In addition, the inactivated bacteria of Bifidobacterium lactis nbk‑W13 also have the effect of enhancing immunity. Experiments have shown that the freeze-dried powder of Bifidobacterium lactis nbk‑W13 and its inactivated powder can enhance immunity by regulating the immune organ coefficient, serum cytokine concentration, antibody concentration and intestinal antioxidant capacity in mice .

Description

technical field [0001] The invention relates to a microorganism and its application, in particular to a bifidobacterium lactis nbk-W13 and its application. Background technique [0002] Bifidobacterium lactis is a beneficial bacterium in the human intestinal tract, which has various functions such as regulating intestinal flora, preventing and treating intestinal diseases, and enhancing the body's immunity. At present, the common Bifidobacterium lactis products on the market are ubiquitous. When the number of viable bacteria is insufficient, the efficacy of the product cannot be fully exerted, and Bifidobacterium lactis has defects such as poor stability and rapid decay of the number of viable bacteria. Therefore, it is urgently needed Development of new strains of Bifidobacterium lactis with multiple functions. Contents of the invention [0003] The object of the present invention is to provide a bifidobacterium lactis nbk-W13 capable of enhancing intestinal mucosal immu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23C9/123A23C9/13A23L33/135A61K35/745A61P37/04A61P1/00C12R1/01
CPCC12N1/20C12N1/04A23C9/1238A23C9/1234A23C9/1307A23L33/135A61K35/745A61P37/04A61P1/00A23V2002/00C12R2001/01C12N1/205A23V2400/123A23V2400/531A23V2400/249A23V2200/3204
Inventor 张阳何慧玲潘芬湛剑龙杨帆
Owner 诺佰克(武汉)生物科技有限公司
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