Bifidobacterium lactis nbk-w13 and its application
A technology of nbk-w13 and bifidobacterium lactis, which is applied in the direction of bifidobacteria, lactobacillus, application, etc., can solve the problems of not fully exerting the efficacy of the product, rapid decay of the number of viable bacteria, poor stability of bifidobacterium lactis, etc. problems, to achieve the effects of non-specific immune function improvement, enhanced immunity, strong acid and bile salt resistance
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Embodiment 1
[0051] Example 1 Screening and Identification of Bifidobacterium lactis nbk-W13
[0052] The feces of healthy infants were collected, ground, diluted with sterile normal saline to a suitable gradient, drawn on the modified MRS agar plate using the four-section line method, and anaerobically cultured in an incubator at 30-42°C for 24-48h. A single colony was selected, examined under a microscope, streaked, and further purified to obtain strain nbk-W13.
[0053] Microbiological characteristics and physiological and biochemical characteristics of strain nbk-W13:
[0054] Cell morphology
[0055] experimental project result Cell morphology Rod s Positive Oxidase Negative Catalase Negative
[0056] Physical and chemical experiment results
[0057]
[0058] The 16S rDNA sequence of strain nbk-W13 is shown in SEQ ID NO:1. According to the identification results of microbiology and molecular biology, the strain nbk-W13 was identified ...
Embodiment 2
[0059] Example 2 Preparation of Bifidobacterium lactis nbk-W13 freeze-dried powder
[0060] The preparation method of bifidobacterium lactis nbk-W13 freeze-dried powder comprises the following steps:
[0061] (1) inoculating Bifidobacterium lactis nbk-W13 into the seed medium for activation to obtain a primary seed liquid;
[0062] (2) inoculating the primary seed liquid into the seed medium for anaerobic cultivation for 8-24 hours to obtain the secondary seed liquid;
[0063] (3) inserting the secondary seed liquid into the fermentation medium to carry out high-density fermentation, centrifuging to get the precipitate, and obtaining the bacteria sludge;
[0064] (4) emulsifying embedding after mixing the bacteria slime and the freeze-drying protective agent to obtain an emulsion;
[0065] (5) The obtained emulsion was freeze-dried and pulverized to obtain the freeze-dried powder of Bifidobacterium lactis nbk-W13.
[0066] In terms of mass percentage, the formula of the see...
Embodiment 3
[0070] Example 3 Preparation of Bifidobacterium lactis nbk-W13 inactivated bacteria powder
[0071] In terms of mass percentage, the formula of the seed medium is: 1.5% lactose, 1.8% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.4% ammonium citrate, 0.02% magnesium sulfate, 0.06% manganese sulfate, 0.06 % L-cysteine hydrochloride, 0.1% Tween 80 and the rest of water, pH 6.8;
[0072] In terms of mass percentage, the formula of the fermentation medium is: 2% lactose, 1.3% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.2% ammonium citrate, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.06 % L-cysteine hydrochloride, 0.1% Tween 80 and balance water, pH 6.4.
[0073] The specific preparation method of bifidobacterium lactis nbk-W13 inactivated bacteria powder is as follows:
[0074] 1) inoculating Bifidobacterium lactis into a test tube containing a seed medium for activation (37°C, 20h) to obtain a first-grade seed liquid;
[0075] 2) Tr...
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