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A method for maintaining the activity of probiotics and its application in solid lipid food

A technology of solid lipid products and probiotics, applied in the fields of application, food science, and preservation of microorganisms, can solve the problems of only about 40%, insignificant effect, complicated production, etc., and achieve high survival rate and excellent vitality maintenance effect , The effect of the simple preparation method

Active Publication Date: 2017-12-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both gastric acid and bile salts have strong antibacterial properties. The results of in vitro simulated digestion tests show that the survival rate of probiotics is usually only about 40% or even lower when tested by acid and bile salt resistance tests.
At present, embedding technology is mainly used to improve the vitality retention effect of probiotics, but this method is limited by the embedding coating material and its application carrier, the production is complicated, and the effect is not significant

Method used

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  • A method for maintaining the activity of probiotics and its application in solid lipid food
  • A method for maintaining the activity of probiotics and its application in solid lipid food
  • A method for maintaining the activity of probiotics and its application in solid lipid food

Examples

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Effect test

Embodiment 1

[0047] Example 1 The probiotic active bacteria slime without glycerol pretreatment makes probiotic chocolate

[0048] A. Draw 200 μL of probiotic bacteria liquid from the glycerol bacteria preservation tube, inoculate it into 1000 mL of MRS broth medium, then carry out activation culture at 37°C under anaerobic conditions for 17 hours, repeat the above activation operation twice, and obtain an activated probiotic Bacterial liquid;

[0049] The probiotics include Lactobacillus fermentum, Lactobacillus cellobiosus, Lactobacillus campylobacter, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus casei, Leuconostoc mesentericus, Pediococcus cerevisiae, Pediococcus pentosacea, Pediococcus lactic acid bacteria, Streptococcus lactis, Streptococcus lactis, Streptococcus thermophilus, Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium infantis, Bifidobacterium longum , Thermophili...

Embodiment 2

[0062] Embodiment 2 Freeze-dried bacteria powder makes probiotic chocolate

[0063] A. Draw 200 μL of probiotic bacteria liquid from the glycerol bacteria preservation tube, inoculate it into 1000 mL of MRS broth medium, then carry out activation culture at 37°C under anaerobic conditions for 17 hours, repeat the above activation operation twice, and obtain an activated probiotic Bacterial liquid;

[0064] The probiotics include Lactobacillus fermentum, Lactobacillus cellobiosus, Lactobacillus campylobacter, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus casei, Leuconostoc mesentericus, Pediococcus cerevisiae, Pediococcus pentosacea, Pediococcus lactic acid bacteria, Streptococcus lactis, Streptococcus lactis, Streptococcus thermophilus, Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium infantis, Bifidobacterium longum , Thermophilic Bifidobacteria.

[0065] The pr...

Embodiment 3

[0080]Example 3 The active probiotics prepared by the method of the present invention are used to prepare probiotic chocolate

[0081] A. Draw 200 μL of probiotic bacteria liquid from the glycerol bacteria preservation tube, inoculate it into 1000 mL of MRS broth medium, then carry out activation culture at 37°C under anaerobic conditions for 17 hours, repeat the above activation operation twice, and obtain an activated probiotic Bacterial liquid;

[0082] The probiotics include Lactobacillus fermentum, Lactobacillus cellobiosus, Lactobacillus campylobacter, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus bulgaricus, Lactobacillus casei, Leuconostoc mesentericus, Pediococcus cerevisiae, Pediococcus pentosacea, Pediococcus lactic acid bacteria, Streptococcus lactis, Streptococcus lactis, Streptococcus thermophilus, Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium infantis, Bifidobacterium long...

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Abstract

The invention discloses a probiotics activity retention method and application thereof to solid-state fatty food, and belongs to the technical field of food processing. According to the probiotics activity retention method, high-concentration glycerol is utilized for dehydration pretreatment of active probiotics by the osmotic equilibrium principle to achieve the purpose of reducing remaining moisture in probiotics. The obtained active bacterial sludge is added in the chocolate temperature-regulating technological process to form a favorable crystal structure together with chocolate fat, and time for probiotics to be active in chocolate is effectively prolonged, so that probiotic chocolate is obtained. The activity retention effect of probiotics in prepared chocolate is approximate to that of a product obtained through processing of freeze-dried bacterial powder. Through adoption of the probiotics activity retention method, a high cost caused by preparation of freeze-dried powder is avoided, energy consumption and equipment investment are low, and the processing cost can be greatly reduced.

Description

technical field [0001] The invention relates to a method for maintaining the activity of probiotics and its application in solid lipid food, belonging to the technical field of food processing. Background technique [0002] Probiotics are a class of active microorganisms that are beneficial to the host. They can colonize the human intestinal tract and reproductive system and produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. The general term for active beneficial microorganisms. In recent years, as people pay more and more attention to their health, probiotics have attracted the attention of many scientific researchers at home and abroad. Their functions include improving digestive system function, enhancing immunity, lowering blood lipids and blood pressure, inhibiting harmful microorganisms, and restoring liver health. functions, and even anti-malignant tumors and many other beneficial effects. However, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04A23G1/44A23G1/42A23L33/135
CPCA23G1/423A23G1/44
Inventor 张灏陈卫赵建新闫博文杨波田丰伟范大明王刚翟齐啸毛丙永
Owner JIANGNAN UNIV
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