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Method for degrading antibiotics in aquaculture wastewater through immobilized pycnoporus

A technology for aquaculture wastewater and antibiotics, applied in the field of antibiotics, can solve the problems of high ammonia nitrogen content, inability to effectively degrade antibiotics, difficult growth of microorganisms, etc., and achieve the effects of fast degradation rate, good cold adaptability, and reduced bacteriostatic effect.

Active Publication Date: 2019-12-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because aquaculture wastewater is usually high-concentration organic sewage, and contains a large amount of suspended solids and ammonia nitrogen, and contains a large amount of antibiotics, it is difficult for ordinary microorganisms to grow and cannot effectively degrade antibiotics.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] strain

[0020] Pycnoporus sp.SYBC-L10 has been preserved in China Center for Type Culture Collection in 2015, with the preservation number CCTCC#NO: M2015020, address: Wuhan University, Wuhan, China.

[0021] culture medium

[0022] PDA medium composition: potatoes 200g L -1 , Glucose 20g L -1 , agar powder 20g L -1 , pH natural;

[0023] Original medium composition (0.5kg / per): wood chips 78% (156g), bran 20% (40g), gypsum 1% (2g), sugar 1% (2g), water 750mL (300g), pH natural;

[0024] Bacteria pack medium composition (1.25kg / per): wood chips 78% (390g), bran 20% (100g), gypsum 1% (5g), fructose 1% (5g), water 750mL (750g), pH natural;

[0025] main instrument

[0026] Vertical steam pressure sterilizer (YXQ-LS-50 Shanghai Boxun); ultra-clean workbench (Suzhou Purification Equipment Co., Ltd.)

example 1

[0027] Example 1 Application of Immobilized Mypopores to Remove Antibiotics in Pig Farm Wastewater

[0028] 1.1 The Pycnoporus sp.SYBC-L10 whose deposit number is CCTCC#NO: M2015020 is immobilized on a solid medium:

[0029] (1) Activation of bacteria: Streak inoculation of the Pycnoporus sp.SYBC-L10 on the PDA plate medium, culture at 30°C for 3-4d;

[0030] (2) Incline cultivation: transfer the strains grown on the plate medium described in step (1) to a PDA incline at 30° C. and cultivate for 4-6 days, until the mycelia cover the incline;

[0031] (3) Original seed culture: transfer the hyphae on the slant described in step (2) together with the culture medium into the original seed medium for cultivation, and culture at 30°C for 12-15 days under static conditions. , Pack the original culture medium in a large-mouth glass bottle, wrap it in gauze kraft paper, and use it after sterilizing at 115°C for 30 minutes;

[0032] (4) Bacteria bag culture: Inoculate the original se...

example 2

[0036] Example 2 Application of immobilized dense porosity to remove antibiotics in chicken farm wastewater

[0037] 2.1 The Pycnoporus sp.SYBC-L10 whose deposit number is CCTCC#NO: M2015020 is immobilized on the solid medium:

[0038] (1) Activation of bacteria: Streak inoculation of the Pycnoporus sp.SYBC-L10 on the PDA plate medium, culture at 30°C for 3-4d;

[0039] (2) Incline cultivation: transfer the strains grown on the plate medium described in step (1) to a PDA incline at 30° C. and cultivate for 4-6 days, until the mycelia cover the incline;

[0040] (3) Original seed culture: transfer the hyphae on the slant described in step (2) together with the culture medium into the original seed medium for cultivation, and culture at 30°C for 12-15 days under static conditions. , Pack the original culture medium in a large-mouth glass bottle, wrap it in gauze kraft paper, and use it after sterilizing at 115°C for 30 minutes;

[0041] (4) Bacteria bag culture: Inoculate the ...

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Abstract

The invention discloses a method for degrading antibiotics in aquaculture wastewater through immobilized pycnoporus. The method is characterized by comprising the following steps that S1, pycnoporus sp.SYBC-L10 is inoculated into a bacterium bag, culturing is conducted for 30-40 days under the still standing condition, and mycelia are overgrown in a culture medium; and S2, firstly, the pH of cultivation waste liquor containing the tetracycline antibiotics or sulfonamide antibiotics is adjusted to be 3.0-6.0, then the cultivation waste liquor is slowly added into the bacterium bag, and still standing for incubation is conducted for a period of time to discharge liquor. According to the method for degrading the antibiotics in the aquaculture wastewater through the immobilized pycnoporus, compared with a traditional physical-chemical method, a method for removing the tetracycline antibiotics and the sulfonamide antibiotics in the cultivation waste liquor efficiently in a low-cost mode isenvironmentally friendly, safe and friendly to the environment, meets the sustainable development demands and has wide application prospects in antibiotic application in the future treatment environment.

Description

technical field [0001] The present invention relates to the technical field of cell immobilization and microbial transformation, in particular to the immobilization of a Mypopore cell and the use of the immobilized cell to catalyze the degradation of antibiotics in aquaculture wastewater. Background technique [0002] In recent years, the rapid development of the aquaculture industry has greatly promoted the improvement of people's living standards. Tetracyclic antibiotics (such as oxytetracycline, chlortetracycline) and sulfonamide antibiotics (such as sulfidesoxine) are used in large quantities without supervision in order to prevent diseases and promote poultry growth in the breeding process. Because these antibiotics can promote the growth of livestock and poultry, and are used as feed additives, they are widely used, and the subsequent breeding wastewater has brought great troubles to people. Because the aquaculture wastewater is usually high-concentration organic wast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F9/14C12N1/14C02F101/34C02F101/38C02F103/20C12R1/645
CPCC02F1/66C02F3/34C02F2101/34C02F2101/38C02F2101/40C02F2103/20C12N1/14
Inventor 廖祥儒田乔鹏赖邓婷孟迪翟李欣管政兵蔡宇杰
Owner JIANGNAN UNIV
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