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Preparation method and application method of thickened oil viscosity reduction and degradation mixed microbial agent

A technology of mixing bacterial agents and application methods, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the problems of insignificant viscosity-reducing effect, high degradation efficiency, and low degradation efficiency, and achieve Realize the effects of viscosity reduction and efficient degradation of petroleum hydrocarbons, high degradation efficiency, and simple preparation process

Active Publication Date: 2020-01-10
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, although people have developed many methods of using microorganisms to degrade and reduce the viscosity of heavy oil, most of them have single functions and have good viscosity reduction effect on heavy oil but low degradation efficiency, or high degradation efficiency but not obvious viscosity reduction effect

Method used

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  • Preparation method and application method of thickened oil viscosity reduction and degradation mixed microbial agent
  • Preparation method and application method of thickened oil viscosity reduction and degradation mixed microbial agent
  • Preparation method and application method of thickened oil viscosity reduction and degradation mixed microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Acinetobacter sp.strain ZX-15, Achromobacter pulmonisstrain PI3-03, Acinetobacter sp.RS206 and Chrobactrum anthropisttrain CON21 domestication, screening and preparation of mixed bacterial agents:

[0049] (1) Take 3g of the soil that has been polluted by heavy oil for a long time in the heavy oil production area of ​​Shengli Oilfield, add it to 150mL enrichment / degradation medium containing 1g / L heavy oil, and culture it in a constant temperature shaker at 37°C for 5 days (160 r / min) Afterwards, 3 mL of the supernatant was transferred to a new enrichment / degradation medium with a heavy oil content of 2 g / L, and cultured again for 5 days. By analogy, gradually increase the concentration of heavy oil to 5g / L, a total of five cycles of domestication.

[0050] (2) Take the culture solution of the last acclimatization cycle and smear the plate, pick colonies with different morphological characteristics, and repeat the streaking until it is confirmed that the colonies in the...

Embodiment 2

[0058] 2. Degradation and viscosity-reducing performance of mixed bacterial agents under different culture conditions

[0059] Inoculate Acinetobacter sp.strain ZX-15, Achromobacter pulmonisstrain PI3-03, Acinetobacter sp.RS206 and Chrobactrum anthropisttrain CON21 in the seed medium respectively, at 37°C, 160r / min Shake at constant temperature for 24 hours to obtain the seed solution, then mix their seed solution according to the volume ratio of 2:2:1:1, inoculate in the degradation medium, and conduct the experiment of the following degradation conditions. Wherein, the degradation medium was 1.0 g of ammonium sulfate, 0.25 g of magnesium sulfate heptahydrate, 2.0 g of sodium nitrate, 4.0 g of potassium dihydrogen phosphate, and 7.6 g of dipotassium hydrogen phosphate were dissolved in 1000 mL of deionized water.

[0060] 1. Different temperature

[0061] Degradation conditions: pH7.5, heavy oil 2g / L, inoculation amount 5% (V / V), sodium chloride 4g / L; temperature: 20°C, 25°C...

Embodiment 3

[0070] Degradation and Viscosity Reduction Performance of Bacteria Agents with Different Mixing Ratio

[0071] Inoculate Acinetobacter sp.strain ZX-15, Achromobacter pulmonisstrain PI3-03, Acinetobacter sp.RS206 and Chrobactrum anthropisttrain CON21 in the seed medium, at 37°C, 160r / min Oscillate at constant temperature for 24 hours to obtain the seed liquid, and the seed liquid is mixed according to the volume ratio according to the following ratio:

[0072]

[0073] The results show that the degradation rate of petroleum hydrocarbons in combination D is the highest, which is 43.84%, and the surface tension is 41.2mN / m. The surface tension of combination E is the lowest, which is 38.7mN / m, and the degradation rate of petroleum hydrocarbons is 40.58%. The surface tension and degradation rate of the two combinations are relatively small. Combination D has the best degradation ability for heavy oil, and has a good viscosity-reducing effect. It is most suitable for biological ...

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Abstract

The invention discloses a preparation method and application method of a thick oil viscosity reduction and degradation mixed microbial agent. The mixed microbial agent is prepared from an Acinetobacter sp. strain ZX-15, an Achromobacter pulmonis strain PI3-03, Acinetobacter sp.RS206 and a chrobactrum anthropi strain CON21. The acinetobacter sp.strain ZX-15 and the Achromobacter pulmonis strain PI3-03 in the preparation method are efficient petroleum hydrocarbon degradation bacteria, and the acinetobacter sp.RS206 and the chrobactrum anthropi strain CON21 are efficient heavy oil viscosity reduction bacteria. The thickened oil viscosity reduction and degradation mixed bacterial agent disclosed by the invention is a mixed bacterial agent constructed by artificial screening, is clear in straincomponent and simple in preparation process, has the high degradation efficiency on thickened oil, has a good viscosity reduction effect, and has good application prospects and industrial productionpotential in an environmental treatment method.

Description

technical field [0001] The invention relates to a preparation method and an application method of a heavy oil viscosity-reducing and degrading mixed microbial agent, belonging to the fields of environmental protection and pollution prevention and control. Background technique [0002] my country has 400,000 tons of heavy oil resources, and the Northeast and North China account for a large proportion of heavy oil reserves, among which the Bohai Oilfield heavy oil accounts for more than 80% of CNOOC crude oil. Due to its high viscosity and heavy components, heavy oil is difficult to be developed and utilized, and it is more difficult to remediate the pollution caused during the mining process. When the microbial method is used for the remediation of heavy oil pollution, the cost is lower and the remediation effect is better; the final product is small molecule water, carbon dioxide or organic matter, and there is no secondary pollution problem; it can be treated in situ and ex...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C02F3/34B09C1/10C12R1/01C12R1/025C02F101/32
CPCB09C1/10C02F3/343C02F2101/32C12N1/20C12N1/36
Inventor 张秀霞辛瑞
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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