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Construction and application of novel fluorescent imprinting sensor based on magnetic metal organic framework (MOF)

A fluorescence sensor and organic framework technology, applied in the field of analytical chemical detection, can solve the problems of unsatisfactory detection range and detection limit, unstable fluorescence, weak fluorescence intensity, etc., achieving satisfactory imprinting effect, simple preparation process, and easy retouching effect

Inactive Publication Date: 2020-01-17
XIANGTAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in our follow-up study, it was found that imprinted polymers with quantum dots exhibit fluorescence instability; imprinted polymers containing organic fluorescent dyes have hidden dangers of photobleaching, and most organic dyes are slightly toxic, which is not necessary Suitable for further use in in vivo studies
Detection using the fluorescence of the virus itself avoids the above problems, but due to its weak fluorescence intensity, the detection range and detection limit obtained are often not ideal

Method used

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  • Construction and application of novel fluorescent imprinting sensor based on magnetic metal organic framework (MOF)
  • Construction and application of novel fluorescent imprinting sensor based on magnetic metal organic framework (MOF)
  • Construction and application of novel fluorescent imprinting sensor based on magnetic metal organic framework (MOF)

Examples

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Effect test

Embodiment 1

[0025] Example 1: Preparation method of a novel virus fluorescent molecular imprinting sensor

[0026] (1) Fe 3 o 4 @MIL-101-NH 2 Synthesis of: First, prepare carboxy-functionalized Fe3O4 particles, 1gFe 3 o 4 , 100mL of isopropanol, 4mL of ultrapure water, ultrasonic treatment for 20min after uniform dispersion. Under mechanical stirring, 20 mL of ammonia water was added, 6 mL of TEOS was added dropwise, and the reaction was carried out in an oil bath at 45° C. for 6 h. Finally washed with distilled water, ethanol, and dried under vacuum at 60 °C to obtain Fe 3 o 4 @SiO 2 The product is ready for use. Dissolve 0.5g of succinic anhydride (SA) and 1mL of 3-aminopropyltriethoxysilane (APTES) in 30mL of acetic acid, sonicate for 5min, and stir at 30°C for 3h. Then add 40mg Fe 3 o 4 @SiO 2 Particles, 20mL acetic acid and 3mL ultrapure water, and the mixture was ultrasonically dispersed and reacted extensively in an oil bath at 30°C for 12h. After washing with water, i...

Embodiment 2

[0030] Example 2: Characterization of the performance, morphology and structure of the MMIPs fluorescent sensor and intermediate products.

[0031] The structure and morphology of all the prepared materials were characterized by Fourier transform infrared spectrometer, X-ray diffractometer and scanning electron microscope. figure 2 for Fe 3 o 4 (a), Fe 3 o 4 @SiO 2 (b), Fe 3 o 4 @MOFs(c) and Fe 3 o 4 Infrared spectra of @MOFs@MIPs(d) particles. about 570cm -1 The absorption peak at is attributed to Fe 3 o 4 Fe-O characteristic peaks. 1096cm -1 The absorption peak at is the stretching vibration peak of Si-O-Si, 793cm -1 The absorption peak at is the vibrational peak of Si-O. This is Fe 3 o 4 @SiO 2 The synthesis provides evidence. The resulting lines all contain these characteristic peaks. After synthesizing the MOF, the infrared spectrum changed again. 1384cm -1 ,1500cm -1 and 1628cm -1 The three absorption peaks represent the Fe 3 o 4 MIL-101-NH for...

Embodiment 3

[0034] Example 3: Application of the fluorescent molecularly imprinted sensor

[0035] The experimental conditions of this example are: the dosage of MMIPs is 22.44 mg / mL, the pH is 7.5, the adsorption time is 15 min, and the temperature is 37°C. The specific embodiment is: take specific concentration of HAV and MMIP in 19 μg / mL PB buffer, adjust the pH of the whole system to 7.5, and measure the fluorescence intensity after shaking and adsorbing at 37° C. for 15 minutes.

[0036] (1) Detection and analysis of different concentrations of HAV by MMIPs fluorescent sensor

[0037] Under the optimal experimental environment, the linear relationship between fluorescence intensity and template virus concentration was studied. Such as Figure 5 As shown, when the template virus concentration is in the range of 20pM-2500pM, the fluorescence intensity has a good linear relationship with the template virus concentration. The linear regression equation is y=251.53x+175.92, R 2 =0.997...

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Abstract

The invention provides preparation of a novel fluorescent imprinting sensor based on a magnetic metal organic framework (MOF), and the novel fluorescent imprinting sensor is successfully used for detection of hepatitis virus a (HAV). In the invention, light emitting MOF (MIL-101-NH2) is introduced to replace quantum dots and organic dyes to serve as output signals. The light emitting MOF materialcan not only be used as the output signals, but also can be used as a carrier. Meanwhile, the metal organic framework has a larger specific surface area, and thus can provide more imprinting sites, thereby improving the response sensitivity of the sensor; due to the combination of ferric oxide particles and the MIL-101-NH2 material, the process of preparing an imprinting polymer is simpler and faster; and a fluorescence analysis method has the advantages of high sensitivity, stronger selectivity, convenient operation and so on. Therefore, by combining the above several advantages, compared with other analysis methods or sensors, the molecular imprinting fluorescent chemical sensor constructed by the invention has good selectivity for template molecules and high detection sensitivity. The results show that the method is simple, convenient and fast, can be used for the qualitative and quantitative detection of HAV, and has important practical potential and significance in biosensing andvirus detection and prevention.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry detection, and in particular relates to the construction and application of a novel fluorescent imprint sensor based on a magnetic metal organic framework. Background technique [0002] In the preparation of traditional fluorescent virus molecularly imprinted materials, researchers often introduce fluorescent materials, such as quantum dots, into the imprinted layer [Zhou J., Yang Y., Zhang C., Chemical reviews, 2015, 115, 11669-11717 .], organic fluorescent dyes, etc. as the output signal, or directly use the fluorescence of the virus itself as the output signal, these works have achieved certain results. However, in our follow-up study, it was found that imprinted polymers with quantum dots exhibit fluorescence instability; imprinted polymers containing organic fluorescent dyes have hidden dangers of photobleaching, and most organic dyes are slightly toxic, which is not necessary S...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/569G01N33/58G01N33/576
CPCG01N21/6428G01N33/56983G01N33/5768G01N33/582Y02A50/30
Inventor 蔡昌群梁琨淞龚行
Owner XIANGTAN UNIV
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