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Double-network structure composite hydrogel as well as preparation method and application thereof

A technology of composite hydrogel and network structure, which is applied in the field of double network structure composite hydrogel medical materials and its preparation, can solve the problems of limited regeneration potential, poor mechanical strength of materials, and lack of injectability.

Active Publication Date: 2020-02-04
DALIAN UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above materials still have the following defects: 1. PRP contains exogenous additives such as anticoagulants, and its regenerative potential is limited; 2. The mechanical strength of the obtained material is poor, and it is not injectable, and the operation is complicated and difficult to be precise. Quantify
However, the degradation period of iPRF is short after implantation, and the growth factor components that induce regeneration are also quickly degraded and inactivated. Therefore, although iPRF has good biological activity and regenerative function, it is still difficult to achieve long-term, controllable factor Release and induce regeneration are limited

Method used

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  • Double-network structure composite hydrogel as well as preparation method and application thereof
  • Double-network structure composite hydrogel as well as preparation method and application thereof
  • Double-network structure composite hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] (1) Preparation of gelatin particles

[0066] Dissolve 5g of gelatin in 100mL of deionized aqueous solution and keep heating to 40°C to obtain a clear and transparent gelatin aqueous solution. Add hydrochloric acid dropwise to adjust the pH value of the solution to 2.5. Add 200, 300, and 350mL of acetone solution dropwise to the above gelatin aqueous solution and Keep heating at 40°C and continuous stirring (1000rpm), the total time of dropping is 20min, add 74μL of crosslinking agent glutaraldehyde (25wt% aqueous solution) to the above-mentioned nanoparticle suspension, crosslinking time 12hrs, after the reaction is completed, Glycine at a concentration of 100 mM was added to the mixture to terminate the end groups of unreacted glutaraldehyde. The nanoparticle suspension was repeatedly centrifuged and resuspended in deionized water. The suspension was freeze-dried at -60°C to obtain dry powder of gelatin nanoparticles.

[0067] The gelatin particles were tested for p...

Embodiment 2

[0095] (1) Use positively charged gelatin type A particle powders with particle diameters of 200, 500, and 1000 nm in Example 1, respectively.

[0096] (2) Extraction of iPRF

[0097] Rabbit blood was drawn through centrifuge tubes without anticoagulant and iPRF was prepared by centrifugation. The specific preparation process is as follows: 1) Fix the New Zealand rabbit with a rabbit holder; 2) Remove the rabbit hair around the middle ear artery of the rabbit, and rub it to fully expand the middle ear artery; 3) Use a blood collection needle and a centrifuge tube to collect 7ml blood from the middle ear artery ; 4) Centrifuge in a centrifuge (300g, 3 minutes), extract 1ml of the supernatant to obtain iPRF.

[0098] (3) 1 mL of centrifuged iPRF and 0.12 g of gelatin nanoparticles were pipetted repeatedly 10 times through a Luer adapter syringe to obtain a double network hydrogel;

[0099] (4) The storage modulus and loss modulus of the above double network hydrogel were obtai...

Embodiment 3

[0103] (1) Use positively charged gelatin type A particle powders with particle diameters of 200, 500, and 1000 nm in Example 1, respectively.

[0104] (2) Extraction of iPRF

[0105]Rabbit blood was drawn through centrifuge tubes without anticoagulant and iPRF was prepared by centrifugation. The specific preparation process is as follows: 1) Fix the New Zealand rabbit with a rabbit holder; 2) Remove the rabbit hair around the middle ear artery of the rabbit, and rub it to fully expand the middle ear artery; 3) Use a blood collection needle and a centrifuge tube to collect 7ml blood from the middle ear artery ; 4) Centrifuge in a centrifuge (300g, 3 minutes), extract 1ml of the supernatant to obtain iPRF.

[0106] (3) 1 mL of iPRF obtained by centrifugation and 0.15 g of gelatin nanoparticles were blown repeatedly 10 times through a Luer adapter syringe to obtain a double network hydrogel;

[0107] (4) The storage modulus and loss modulus of the above double network hydrogel...

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Abstract

The invention relates to double-network structure composite hydrogel. Gelatin nano-colloidal particles form a first colloidal gel network under the electrostatic interaction and the hydrogen-bond interaction, and fibrinogen in iPRF forms a second gel network under the thrombin action. The double-network structure composite hydrogel provided by the invention is prepared by the following steps: adding a non-anticoagulation medicine in a fresh blood sample, wherein the blood can be derived from an autologous or allogeneic implanted patient; taking all on a top layer after low-speed centrifugal operation and blending the yellow liquid with gelatin particle dry powder; injecting the mixture into a mold for molding or in a tissue defect site to realize tissue filling; and waiting for not more than 2000 seconds at room temperature or body temperature to perform complete solidification to obtain a hydrogel biological medical material which has a double network structure and enhanced strength and toughness. The preparation method provided by the invention is simple and convenient and is favorable for clinical promotion. The double-network structure composite hydrogel is a regenerated, ideal, injectable and moldable biological medical material for human tissue and organ defect reconstruction.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a double network structure composite hydrogel medical material and its preparation method and application. Background technique [0002] Achieving damage repair of human tissues / organs is a key problem in clinical medicine. However, autologous tissue, allogeneic tissue or xenogeneic tissue is still the main method for clinical treatment of human tissue / organ repair. Taking bone repair as an example, the clinical "golden" treatment standard for bone repair is the patient's autologous bone graft filling. Since there is no immune rejection of autologous tissue / organ and it is usually well vascularized, the repair effect is remarkable. For example, the best clinical bone repair plan for bone defects is to take bone from the patient's own iliac crest and transplant it to the patient's bone defect site; although this plan has good clinical repair effect, its defects are ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/10A61L27/12A61L27/22A61L27/52A61L27/54A61L27/50
CPCA61L27/10A61L27/12A61L27/222A61L27/225A61L27/3608A61L27/3616A61L27/507A61L27/52A61L27/54A61L2400/06A61L2400/12A61L2430/02A61L2430/06A61L2430/34C08L89/00
Inventor 王华楠陈楷文陈陶木志翔
Owner DALIAN UNIV OF TECH
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