Preparation method and application of injectable hemostatic adhesive hydrogel based on gelatin particles

A hydrogel and gelatin technology, which is applied in the field of materials science and biomedical materials, can solve the problems of difficult sealing, the inability of cells and tissues to provide active regulation and response, and the difficulty of minimally invasive applications, so as to achieve the effect of improving mechanical strength

Inactive Publication Date: 2022-05-31
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional trauma integration methods and materials have certain advantages, there are still 30% of acute trauma or wounds that are difficult to seal, which cannot be sealed by traditional trauma materials
At the same time, traditional wound suture will increase the risk of bacterial infection, and it is difficult

Method used

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  • Preparation method and application of injectable hemostatic adhesive hydrogel based on gelatin particles
  • Preparation method and application of injectable hemostatic adhesive hydrogel based on gelatin particles
  • Preparation method and application of injectable hemostatic adhesive hydrogel based on gelatin particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of gelatin particles

[0033] 10g of A-type gelatin was dissolved in 100mL of deionized aqueous solution, and kept heated to 50°C to obtain a clear and transparent aqueous gelatin solution. Hydrochloric acid was added dropwise to adjust the pH of the solution to 2.5, and 240 and 350mL of acetone solution were added dropwise to the above gelatin solution. In and keep heating at 50 ° C and continuous stirring (1000 rpm), the total dropwise addition time is 40 min, and 84 μL of the crosslinking agent glutaraldehyde (25wt% aqueous solution) is added to the above nanoparticle suspension. The crosslinking time is 16h, and the reaction is completed. Then, 100 mM glycine was added to the mixture to terminate unreacted end groups of glutaraldehyde. The nanoparticle suspension was repeatedly centrifuged and resuspended in deionized water. The suspension was freeze-dried at -80°C to obtain dry powder of gelatin nanoparticles.

[0034] The particle size and surfac...

Embodiment 2

[0046] (1) Preparation of gelatin particles

[0047] Positively charged gelatin nanoparticles prepared in Example 1 were used.

[0048] (2) Grafting of polyethylene glycol polymer groups

[0049] Use polyethylene glycol diacrylate with molecular weights of 0.6kDa and 20kDa (purchased from China Sigma-Aldrich Chemical Reagent Company)

[0050] (3) Dissolve 0.1g of 0.6kDa and 20kDa polyethylene glycol diacrylate and 0.005g of 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone photoinitiator respectively In 1 mL of aqueous solution, 0.2 g of gelatin particles were repeatedly pipetted through a luer adapter syringe for 10 times to obtain a prepolymerized colloidal gel with injectable and self-healing properties such as figure 2 shown, figure 1 It is shown that the prepolymerized colloidal gel has shear-thinning mechanical properties when no polymer network is formed, and optical images show that it has excellent injectable properties, figure 2 It is shown that the prepolym...

Embodiment 3

[0057] (1) Preparation of gelatin particles

[0058] Positively charged gelatin nanoparticles prepared in Example 1 were used.

[0059] (2) Grafting of polyethylene glycol polymer groups

[0060] Use polyethylene glycol diacrylate with molecular weights of 0.6kDa and 20kDa (purchased from China Sigma-Aldrich Chemical Reagent Company)

[0061] (3) Dissolve 0.05g of 0.6kDa, 20kDa polyethylene glycol diacrylate and 0.005g of 2-hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone photoinitiator respectively In 1 mL of aqueous solution, 0.2 g of gelatin particles were repeatedly pipetted through a luer adapter syringe for 10 times to obtain an injectable, self-healing prepolymerized colloidal gel;

[0062] (4) The above prepolymerized colloidal gel is at 365nm, 50mw / cm 2 Double-network hydrogels were obtained by crosslinking under UV light for 20 s.

[0063] (5) The storage moduli and loss moduli (Table 4) of the above composite hydrogels were obtained using the time-sweep mode o...

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Abstract

The invention relates to a novel tissue hemostasis binding material based on nano colloid assembly, and discloses gelatin particle/polyethylene glycol-based high-strength hydrogel as well as a preparation method and application thereof. The binding material and the tissue can construct a multi-field coupling cross-linked network, and rapid and stable wet tissue binding is achieved. The colloidal gel has shear thinning and self-repairing effects, and on the basis of reversible non-covalent bonds (such as static electricity and hydrogen-bond interaction) among nano-particles, the colloid realizes the self-repairing effect through self-assembly so as to be quickly cured, so that a wound is quickly blocked, and the wound surface is closed for hemostasis; the photosensitive group modified on the surface of the nano colloid is further subjected to covalent crosslinking with a tissue matrix through photo-initiation, and mechanical interlocking, molecular entanglement and covalent bond combination with tissues are rapidly formed, so that stable tissue adhesion is realized.

Description

technical field [0001] The invention belongs to the fields of material science and biomedical materials, and in particular relates to an injectable, hemostatic and adhesive hydrogel material assembled from gelatin particles / polyethylene glycol based macromolecules and a preparation method and application thereof. Background technique [0002] At present, 50% of the deaths of war personnel and more than 80% of traffic accident deaths are caused by acute traumatic blood loss. Therefore, how to achieve rapid, effective and safe sealing and hemostasis of trauma has extremely important significance and clinical value. Taking clinical demand as an example, according to statistics, the global clinical demand for biomedical materials for traditional trauma integration such as trauma sutures and staples has reached 15 billion US dollars per year. Although traditional wound integration methods and materials have certain advantages, there are still 30% of acute wounds or wounds that ar...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/06A61L24/10A61L27/18A61L27/22A61L27/50A61L27/52C08J3/075C08J3/24C08J3/28C08L89/00C08L71/02
CPCA61L24/0031A61L24/104A61L24/06A61L24/001A61L27/222A61L27/18A61L27/50A61L27/52C08J3/075C08J3/246C08J3/28A61L2400/04A61L2400/06A61L2430/02A61L2430/06C08J2389/00C08J2371/02C08J2471/02C08L89/00C08L71/02
Inventor 何炜杨松王华楠陈楷文
Owner DALIAN UNIV OF TECH
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