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Antibacterial leakproof endocranium repairing piece and preparation method thereof

A dura mater and patch technology, which is applied in the field of antibacterial and anti-leakage dura mater patch and its preparation, can solve the problems of weakening, easily causing epilepsy, increasing calvarial bone adhesion, etc., achieves good tissue compatibility, prevents cerebrospinal fluid Leakage, reduce the effect of intracranial infection

Inactive Publication Date: 2020-02-07
SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the artificial dura mater prepared by this method damages the natural structure of the acellular matrix, weakens the advantages of biofilm materials that are easy to guide the growth of new tissues, increases the adhesion with the calvaria, and easily causes epilepsy.

Method used

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  • Antibacterial leakproof endocranium repairing piece and preparation method thereof
  • Antibacterial leakproof endocranium repairing piece and preparation method thereof
  • Antibacterial leakproof endocranium repairing piece and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The invention provides an antibacterial and anti-leakage dura mater patch, which comprises a repair layer, an antibacterial anti-seepage and anti-adhesion layer, and the anti-bacterial anti-seepage and anti-adhesion layer is spread on the repair layer. Such as figure 1 Shown is the scanning electron microscope image of the bacteriostatic biological dura mater patch coating.

[0030] The spreading and repairing layer of the antibacterial anti-seepage and anti-adhesion layer is spread on the repairing layer through coating, casting or electrospinning technology; compared with other methods, the coating method is easy to operate and has a low waste rate. The cost is low, and there is no significant difference in the performance of the two-layer composite structure when it is integrated with the acellular matrix, so the most energy-saving coating method is preferred in the present invention. The coating prepared by the coating method can achieve airtight sealing, prevent c...

Embodiment 2

[0036] The preparation method of the antibacterial anti-leakage dura mater patch described in embodiment 1 comprises the steps:

[0037] Step 1: Remove excess fat and subsidiary tissues in the animal membrane tissue, and wash repeatedly with purified water or saline until the blood color is removed; then extract with organic reagents at a ratio of 1:5 to 1:15 (w / v), at room temperature, 100-140 rpm, shake and degrease for 2-6 hours, replace the organic reagent and continue shaking and degreasing for 10-20 hours; discard the organic reagent, add purified water at 1:20 (w / v), room temperature, 100-120rpm, shake and wash for 5- 10 times, 10min / time, to obtain degreased animal membrane tissue; add alkaline solution to degreased animal membrane tissue at a material-liquid ratio of 1:2~1:10 (w / v), room temperature, 100~120rpm, oscillate 20-60min, wash and repeat three times to obtain the decellularized matrix;

[0038] Step 2: Prepare the natural polymer material into a natural pol...

Embodiment 3

[0058] Step 1: Animal membrane tissue pretreatment: The selected bovine pericardium refers to the selection of qualified slaughterhouses and the selection of healthy cattle. Tear off the easy-to-tear fat attached to the bovine pericardium, then spread the bovine pericardium on a stainless steel plate, scrape off the remaining fat with a card, and cut off the parts that are difficult to remove fat or impurities on the edge; add purified The bovine pericardium was washed repeatedly with water until the surface was free of blood and other attachments.

[0059] Step 2: Add isopropanol in a fume hood at a material-to-liquid ratio of 1:8 (weight-to-volume ratio), degrease by shaking at 120 rpm at room temperature for 4 h, replace the isopropanol, and continue degreasing by shaking at 120 rpm at room temperature for 11 h; discard Isopropanol and purified water were used to wash the isopropanol attached to the bovine pericardium and the surface of the Lock Box, and purified water was ...

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Abstract

The invention relates to antibacterial leakproof endocranium repairing piece. The antibacterial leakproof endocranium repairing piece comprises a repairing layer and an antibacterial leakproof and antiblocking layer, wherein the antibacterial leakproof and antiblocking layer is tiled on the repairing layer. The leakproof and antiblocking layer of the antibacterial leakproof endocranium repairing piece can form a humid and smooth gelatin surface in bodies, the brain tissue can be protected, generation of blemish and epilepsy can be avoided, moist heal environment can be provided for wound parts, maintaining of cell viability is facilitated, and the healing of the wound parts is facilitated. The repairing layer is not cross-linked, so that a natural three-dimensional space structure is reserved, and cell ingrowth is facilitated. In addition, the antibacterial leakproof endocranium repairing piece is simple in preparation method, and can effectively prevent products from being polluted inthe processing course. The product quality is easy to control, the raw materials are broad in sources, the cost is low, and cost for mass production is reduced.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to an antibacterial anti-leakage dura mater patch and a preparation method thereof. Background technique [0002] The dura mater is a thick and tough double membrane tissue on the surface of the brain tissue, which is an important barrier to protect the brain tissue and prevent the cerebrospinal fluid from communicating with the outside world. Traumatic brain trauma, intracranial tumors, cerebrovascular diseases, and congenital diseases can all cause dural defects, and if the dural defects caused by different reasons are not repaired in time, they will cause cerebrospinal fluid leakage and intracranial infection, resulting in brain tissue damage. Adhesion with the surrounding tissue, epilepsy and other complications. Therefore, in order to protect the brain tissue and restore the normal physiological and anatomical structure, the use of artificial repair ma...

Claims

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Application Information

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IPC IPC(8): A61F2/02A61L27/36A61L27/20A61L27/54A61L27/56A61L27/58A61L27/50
CPCA61F2/02A61L27/3633A61L27/3687A61L27/3675A61L27/20A61L27/54A61L27/56A61L27/58A61L27/50A61F2240/001A61L2300/232A61L2300/404A61L2430/32
Inventor 李亚婧黄文涛
Owner SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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