A silicon-based elastic nanofiber bone repair material and its preparation method and application

A nanofiber and bone repair technology, applied in the direction of fiber chemical characteristics, rayon manufacturing, prosthesis, etc., can solve problems such as low mechanical strength, and achieve the effect of simple preparation method, easy preparation, and promotion of bone defect repair

Active Publication Date: 2020-08-18
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the shortcomings of the existing nanofiber scaffolds such as low mechanical strength, the purpose of the present invention is to provide a silicon-based elastic nanofiber bone repair material and its preparation method and application

Method used

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  • A silicon-based elastic nanofiber bone repair material and its preparation method and application
  • A silicon-based elastic nanofiber bone repair material and its preparation method and application
  • A silicon-based elastic nanofiber bone repair material and its preparation method and application

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preparation example Construction

[0040] like figure 1 Shown, preparation method of the present invention specifically comprises the following steps:

[0041] 1) Synthesis of polycitrate-polyethyleneimine copolymer-miRNA carrier. Add POCG-PEI (i.e. PCEE) and miRNA with a nitrogen-to-phosphorus ratio of (2-16):1 to 10 μL, 50 mM, pH 7.4 HEPES buffer, and incubate in a water bath at 37°C for 30 minutes to form stable PCEE@miRNA nanocomposites.

[0042] Preferably, the PCEE in step 1) is POCG-PEI 1.8K; preferably, the nitrogen-phosphorus ratio of PCEE and miRNA is (4-10):1; miRNA is miR-5106, miR-29b and miR-26a / b .

[0043] 2) Synthesis of silicon-containing polyester and biomedical polyester polymer (SPE-BPE). Dissolve silicon-containing polyester (SPE) and biomedical polyester (BPE) with a mass ratio of 1: (1-5) in a volume ratio of 1: (0.08-0.15) dichloromethane (DMF) and dimethylmethylene The prepolymer was obtained in a mixed solvent of sulfone (DMSO), and 1,6-hexamethylene diisocyanate (HDI) with a mol...

Embodiment 1

[0051] 1) Synthesis of polycitrate-polyethyleneimine copolymer-miRNA carrier. PCEE and miR-5106 with a nitrogen to phosphorus ratio of 4:1 were added to 10 μL, 50 mM, pH 7.4 HEPES buffer, and incubated in a 37°C water bath for 30 minutes to form a stable nanocomplex.

[0052] 2) Synthesis of silicon-containing polyesters and biomedical polyester polymers. Dissolve SPE and BPE with a mass ratio of 1:2.3 in a mixed solvent of DMF and DMSO with a volume ratio of 1:0.08 to obtain a prepolymer, add HDI with a molar ratio of 1:2 to SPE, and add a mass ratio of 0.1% The stannous octoate catalyst obtains the SPE-BPE polymer (PP (0.3)) that SPE accounts for (SPE+BPE) mass ratio and is 0.3.

[0053] 3) Synthesis of three-dimensional silicon-based elastic nanofiber bone repair materials. SPE-BPE and PCEE@miRNA were mixed at a mass ratio of 1:0.005, and the electrospinning process of the mixed solution was performed using a 5mL syringe equipped with a 20-gauge needle, using a voltage of...

Embodiment 2

[0055] 1) Synthesis of polycitrate-polyethyleneimine copolymer-miRNA carrier. PCEE and miR-5106 with a nitrogen to phosphorus ratio of 4:1 were added to 10 μL, 50 mM, pH 7.4 HEPES buffer, and incubated in a 37°C water bath for 30 minutes to form a stable nanocomplex.

[0056] 2) Synthesis of silicon-containing polyesters and biomedical polyester polymers. Dissolve SPE and BPE with a mass ratio of 1:1.5 in a mixed solvent of DMF and DMSO with a volume ratio of 1:0.08 to obtain a prepolymer, add HDI with a molar ratio of 1:2 to SPE, and add a mass ratio of 0.1% The stannous octoate catalyst obtains the SPE-BPE polymer (PP (0.4)) that SPE accounts for (SPE+BPE) mass ratio and is 0.4.

[0057] 3) Synthesis of three-dimensional silicon-based elastic nanofiber bone repair materials. SPE-BPE and PCEE@miRNA were mixed at a mass ratio of 1:0.005, and the electrospinning process of the mixed solution was performed using a 5mL syringe equipped with a 20-gauge needle, using a voltage of...

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Abstract

The invention discloses a silicon-based elastic nano-fiber bone repairing material, as well as a preparation method and application thereof. The preparation method comprises the following steps: adding a PCEE polymer and miRNA into an HEPES buffer solution, and performing incubation in water bath to form stable nano-compound PCEE@miRNA; dissolving a silicon-containing polyester and biomedical polyester into a solvent to obtain prepolymer, adding silicon-based hybridized poly(diol citrate) 1,6-hexamethylene diisocyanate into the prepolymer, adding a catalyst into the mixture, and performing a reaction to obtain a SPE-BPE polymer; mixing the SPE-BPE polymer and PCEE@miRNA, performing electrostatic spinning, and performing crosslinking to obtain a miRNA-containing three-dimensional silicon-based elastic nano-fiber bone repairing material. The three-dimensional silicon-based elastic nano-fiber bone repairing material prepared by the method has excellent photoluminescence, mechanical performance and biocompatibility and has an effect of promoting bone defect repairing.

Description

technical field [0001] The invention belongs to the technical field of degradable biomedical materials, and in particular relates to a silicon-based elastic nanofiber bone repair material and its preparation method and application. Background technique [0002] Rapid and effective bone tissue regeneration techniques, especially for the repair of critical size (≥5 mm) bone defects, remain a challenge in orthopedic surgery. The gold standard for the treatment of bone defects is still autologous bone grafting, but the risk of infection and the great pain of bone cutting and grinding cannot be ignored. Bone tissue engineering can provide a feasible method for the preparation of bone graft substitutes by promoting the migration, proliferation and osteogenic differentiation of stem cells or bone cells. Biomaterial scaffolds have been playing an important role in providing extracellular matrix for tissue repair, especially in damaged tissues. In order to promote the repair and re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/50A61L27/56A61L27/54D01F8/16D01F8/14D01F1/10
CPCA61L27/18A61L27/50A61L27/54A61L27/56A61L2300/258A61L2300/412A61L2430/02D01F1/10D01F8/14D01F8/16C08L87/005C08L75/06C08L67/00
Inventor 雷波于萌杜玉章
Owner XI AN JIAOTONG UNIV
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