Unlock instant, AI-driven research and patent intelligence for your innovation.

3D printing guar gum hydrogel stent and preparation method thereof

A technology for printing melon and guar gum, which is used in medical science, tissue regeneration, prosthesis, etc., can solve problems such as blockage, cell damage by extrusion force, and inability to meet repair requirements, and achieves simple response requirements, high precision, The effect of good chondrocyte compatibility and bioactivity

Inactive Publication Date: 2020-02-07
GUANGXI MEDICAL UNIVERSITY
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate and hyaluronic acid have been widely used as natural materials for 3D bioprinting, but there are still some defects in the construction of tissue engineered cartilage: sodium alginate solution is often blocked during the printing process due to its high viscosity. The printing needle of the traditional 3D printer, so the extrusion force is too large and the cells are damaged; the 3D printed hyaluronic acid scaffold is often unable to meet the repair requirements due to the rapid degradation rate in the body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 3D printing guar gum hydrogel stent and preparation method thereof
  • 3D printing guar gum hydrogel stent and preparation method thereof
  • 3D printing guar gum hydrogel stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Follow the steps below to prepare 3D printed guar gum hydrogel scaffolds:

[0038] S1. Under normal temperature, 0.5g guar gum is completely dissolved in 25mL ultrapure water to obtain an aqueous solution of guar gum. After adding 0.5mL methacrylic anhydride, stir fully. The molar ratio of methacrylic anhydride to guar gum is approximately equal to 1. Adjust the pH value of the system to 8 with a sodium hydroxide solution with a concentration of 5 mol / L, continue the reaction for 2 hours, dialyze the solution obtained by the reaction with ultrapure water for 5 days, and obtain guar gum methyl after complete freeze-drying Acrylate;

[0039] S2. In a sterile environment, according to the dosage ratio of 1g:50mL, guar gum methacrylate is completely dissolved in the aseptic PBS solution containing photoinitiator 2959 with a concentration of 0.1%w / v to obtain a concentration of 2 %w / v sol, according to 1mL: 1×10 7 The dosage ratio of the sol and chondrocytes is evenly mixe...

Embodiment 2

[0041] Follow the steps below to prepare 3D printed guar gum hydrogel scaffolds:

[0042]S1. At room temperature, completely dissolve 0.5g of guar gum in 50mL of ultrapure water to obtain an aqueous solution of guar gum, add 1mL of methacrylic anhydride and stir well, the molar ratio of methacrylic anhydride to guar gum is approximately equal to 2 , adjust the pH value of the system to 9 with a sodium hydroxide solution with a concentration of 5mol / L, continue the reaction for 4 hours, dialyze the solution obtained by the reaction for 6 days with ultrapure water, and obtain guar gum methacrylic acid after complete freeze-drying ester;

[0043] S2. In a sterile environment, according to the dosage ratio of 3g:100mL, guar gum methacrylate is completely dissolved in the sterile PBS solution containing photoinitiator 2959 with a concentration of 0.5%w / v to obtain a concentration of 3 %w / v sol, according to 1mL: 1×10 7 The dosage ratio of the sol and chondrocytes is evenly mixed ...

Embodiment 3

[0045] Follow the steps below to prepare 3D printed guar gum hydrogel scaffolds:

[0046] S1. Under normal temperature, completely dissolve 0.5g guar gum in 50mL ultrapure water to obtain an aqueous solution of guar gum, add 1.5mL methacrylic anhydride and stir fully, the molar ratio of methacrylic anhydride to guar gum is approximately equal to 3. Adjust the pH value of the system to 10 with a sodium hydroxide solution with a concentration of 5 mol / L, continue the reaction for 6 hours, dialyze the solution obtained by the reaction with ultrapure water for 7 days, and obtain guar gum methyl after complete freeze-drying Acrylate;

[0047] S2. In a sterile environment, according to the dosage ratio of 6g:100mL, guar gum methacrylate is completely dissolved in the aseptic PBS solution containing photoinitiator 2959 with a concentration of 1%w / v to obtain a concentration of 6 %w / v sol, according to 1mL: 1×10 7 The dosage ratio of the sol and chondrocytes is evenly mixed to make ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Compression modulusaaaaaaaaaa
Login to View More

Abstract

The invention provides a 3D printing guar gum hydrogel stent. A preparation method comprises the following steps: S1, dissolving guar gum in ultrapure water at normal temperature to prepare a guar gumaqueous solution, adding methacrylic anhydride, fully stirring the materials, adjusting a pH value of a system to 8-10, continuously reacting the materials for 2-6 hours, dialyzing the solution, andfreeze-drying to obtain guar gum methacrylate; S2, in a sterile environment, dissolving guar gum methacrylate in a sterile PBS solution containing a photoinitiator to prepare sol, evenly mixing the sol and cartilage cells, performing extrusion type printing through a 3D biological printer to form a stent, irradiating the stent with ultraviolet light to be crosslinked, and obtaining the 3D printingguar gum hydrogel stent. The invention further provides a preparation method of the 3D printing guar gum hydrogel stent. The 3D printing guar gum hydrogel stent provided by the invention is expectedto become an ideal cartilage tissue engineering stent and is expected to be applied to regeneration and repair of cartilage defects clinically.

Description

technical field [0001] The invention relates to a 3D printing guar gum hydrogel support and a preparation method thereof. Background technique [0002] Tissue engineering provides a new treatment method for the regeneration and repair of human tissues and organs, including common material processing and molding technologies such as electrospinning and 3D printing technology. The former mainly provides a two-dimensional microenvironment for cells, which It is far from satisfying the three-dimensional microenvironment of bionic tissues and organs. 3D printing technology can print various functional and individualized tissues or organs by precisely placing cells and biological materials at specific positions in the preset space, and provides unprecedented capabilities and versatility to bridge engineering It is possible to construct individualized functional cartilage through the combination of customized tissue and native tissue. However, the lack of individualized biochemic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/20A61L27/38A61L27/50A61L27/52A61L27/56B33Y70/10B33Y80/00
CPCA61L27/20A61L27/3817A61L27/50A61L27/52A61L27/56A61L2430/06B33Y70/00B33Y80/00C08L5/00
Inventor 高明郑立覃再嫩杨屹峰赵劲民
Owner GUANGXI MEDICAL UNIVERSITY