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Probiotic preparation and application thereof

A probiotic preparation and application technology, applied in probiotic preparations, feeds, pharmaceutical compositions, probiotic preparations or pharmaceutical compositions in the field of pharmaceutical preparation, can solve problems affecting the digestion, absorption and secretion capacity of the small intestine, and the intestinal microecology of animals. Unbalance, decreased immunity and other problems, to enhance the intestinal barrier, increase body weight and food intake, and promote the growth of villi

Pending Publication Date: 2020-02-21
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stress of these weaning stages will activate some physiological mechanisms, resulting in reduced feed intake of piglets, reduced activity of digestive enzymes in the gastrointestinal tract, affecting the digestion, absorption and secretion capabilities of the small intestine, reduced intestinal barrier function, intestinal flora disorder and even diarrhea, reduce production performance
At present, antibiotics are mainly used to alleviate the above-mentioned gastrointestinal reactions of piglets after weaning, but the abuse of antibiotics in animal feed will not only lead to the imbalance of intestinal microecology of animals, but also easily cause endogenous infection of animals, decreased body immunity, and antibiotics in meat products Residues may also indirectly threaten human health through the food chain
[0003] Therefore, alternatives to antibiotics to alleviate gastrointestinal reactions in piglets at weaning need to be investigated

Method used

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  • Probiotic preparation and application thereof
  • Probiotic preparation and application thereof
  • Probiotic preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In this example, by adding probiotic compound preparations of different strain combinations to the diet of weaned piglets, it was determined that it can improve the growth performance of piglets, weaning diarrhea (PWD), intestinal morphology, blood routine and serum biochemical indicators impact, as follows:

[0040] 1. Experimental materials

[0041] 1.1 Strain and treatment of probiotic compound preparation

[0042] Positive control group: Enterococcus faecium (DSM 7134, 5x10 9 ), Bacillus subtilis (AS1.836, 8x10 10 ) and Saccharomyces cerevisiae (ATCC28338, 1x10 10 ).

[0043] Experimental group: Enterococcus faecium (DSM 7134, 5x10 9 ), Bacillus subtilis (AS1.836, 8x10 10 ) and Lactobacillus paracasei L9 (CGMCC 9800, 1x10 10 ).

[0044] Strain treatment: different strains were inoculated in the corresponding culture medium, cultivated to about 10 11 CFU / ml, and then use a fermenter to expand the culture, centrifuge at 4000rpm for 15min at room temperature, ...

Embodiment 2

[0099] In this embodiment, taking the three groups of weaned piglets in Example 1 as the research object, the influence of the positive control group and the experimental group probiotic compound preparation in Example 1 on the immune performance of weaned piglets was studied, and by measuring the blood of weaned piglets Immune indicators and intestinal mucosal immune indicators were verified.

[0100] 1. Experimental method

[0101] 1.1. Determination of immunoglobulin

[0102] On the morning of the 21st day of the experimental period, the weaned piglets of Example 1 were randomly selected from 6 weaned piglets in each group, and the blood was collected from the anterior vena cava on an empty stomach, and packed in vacuum coagulation-promoting tubes. Centrifuge at a speed of r / min for 10 min, take the supernatant, aliquot and store in a -20°C refrigerator for testing. The content of serum immunoglobulin IgA, IgM and IgG was determined by immunonephelometric method.

[0103...

Embodiment 3

[0134] In this example, the three groups of weaned piglets in Example 1 were taken as the research objects, and the effects of the positive control group and the experimental group probiotic compound preparation in Example 1 on the intestinal health of weaned piglets were studied.

[0135] 1. Experimental method

[0136] 1.1 Fecal DNA extraction and high-throughput sequencing

[0137] (1) DNA extraction

[0138] The feces were placed in a container with a stabilizing solution (RNAlater, containing 20 ml [0.5M] EDTA solution and 25 ml [1M] sodium citrate solution) of saturated ammonium sulfate solution [pH 5.2]. The total microbial DNA in feces was extracted by phenolform extraction.

[0139] (1) Shake the feces soaked in RNAlater on a vortex shaker, absorb 200 μl of the suspension with a sterilized snipped pipette tip, add 2 mL of sterilized centrifuge tube, add 500 μl of PBS buffer, after balancing, centrifuge at low temperature The machine was centrifuged at 14000rpm for ...

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Abstract

The invention discloses a probiotic preparation and application thereof. The probiotic preparation comprises lactobacillus paracasei, wherein the biological accession number of the lactobacillus paracasei is CGMCC No.9800, and the lactobacillus paracasei is separated from the intestinal tract of a healthy person. The probiotic preparation is suitable for animals, is especially suitable for pigletsand is particularly suitable for weaning piglets. The probiotic preparation can improve weight and food intake, lowers a meat to fodder ratio, accelerates intestinal villi growth, increases a crypt depth of the colon, deeply alleviates diarrhea, enhances an intestinal tract barrier function, and has a good flora regulation and immunization effect so as to accelerate growth.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to probiotic preparations and applications thereof, more specifically to the use of probiotic preparations, feeds, pharmaceutical compositions and probiotic preparations or pharmaceutical compositions in the preparation of medicines. Background technique [0002] In the current commercial pig farming, the weaning stage is a critical stage that affects pig production and breeding income, because piglets are weaned earlier in production, and piglets face severe stress during the weaning period, such as sudden separation from the sow, transportation pressure, food Change from liquid breast milk to solid rations with different nutritional content, the stress of co-existing piglets from other litters, and unfamiliar environments. The stress of these weaning stages will activate some physiological mechanisms, resulting in reduced feed intake of piglets, reduced activity of digestive e...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K50/30A23K50/60A61K35/747A61P1/12A61P1/00C12N1/20C12R1/225C12R1/01C12R1/125
CPCA23K10/18A23K50/30A23K50/60A61K35/747A61P1/12A61P1/00C12N1/20
Inventor 任发政卢旭红张明赵亮
Owner CHINA AGRI UNIV
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