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A strain of Bacillus ayerbordella and its application in the degradation of acrylic acid

A technology of Bacillus solani and Bacillus, which is applied to Bacillus ayerbodotes CY and its application in acrylic acid degradation, can solve the problems of few reports, limited promotion, and low energy consumption.

Active Publication Date: 2021-04-20
ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Catalytic wet oxidation has the advantages of high removal efficiency, low energy consumption, and less secondary pollution, and is a relatively clean treatment technology. However, there are problems such as high operating costs, difficult control, and potential pollution of heavy metal catalysts; supercritical water oxidation The method has the advantages of short reaction time, complete removal of pollutants, small footprint, clean and environmental protection, etc. However, equipment corrosion and inorganic salt deposition caused by high-concentration dissolved oxygen, high temperature and high pressure are the main problems faced by this technology; fiber adsorption method, The ion exchange fiber method and photoelectron wave decomposition method have the characteristics of mild reaction conditions, fast reaction speed, and high removal efficiency, but there are few in-depth studies at present, and the high processing cost limits the promotion of their practical engineering applications.
The use of biological methods to treat acrylic acid wastewater has the characteristics of low energy consumption, mild reaction conditions and high degradation efficiency, but there are few reports on strains with acrylic acid degradation ability at home and abroad.

Method used

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  • A strain of Bacillus ayerbordella and its application in the degradation of acrylic acid
  • A strain of Bacillus ayerbordella and its application in the degradation of acrylic acid
  • A strain of Bacillus ayerbordella and its application in the degradation of acrylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation, purification and identification of Bacillus aryabhattai CY

[0023] 1. Isolation and purification of Bacillus aryabhattai CY

[0024] Bacillus aryabhattai CY is screened from the sludge of the wastewater treatment pond of Zhejiang Satellite Energy Co., Ltd. The specific steps are as follows:

[0025] Acrylic acid liquid basal medium, the preparation method is as follows: NH 4 Cl 540mg·L -1 , K 2 HPO 4 1000mg·L -1 , KH 2 PO 4 1000mg·L -1 , NaCl 500mg·L -1 , yeast extract powder 1000mg·L -1 , acrylic acid 100mg·L -1 , MgSO 4 ·7H 2 O 4mg·L -1 , CaCl 2 1mg·L -1 , CuSO 4 1mg·L -1 , FeSO 4 1mg·L -1 , MnSO 4 1mg·L -1 , the solvent is water, pH 7.0, sterilized at 121°C for 20min.

[0026] Acrylic solid selection medium: NH 4 Cl 540mg·L -1 , K 2 HPO 4 1000mg·L -1 , KH 2PO 4 1000mg·L -1 , NaCl 500mg·L -1 , yeast extract powder 1000mg·L -1 , acrylic acid 100mg·L -1 , MgSO 4 ·7H 2 O 4 mg L -1 , CaCl 2 1mg·L -1 , C...

Embodiment 2

[0039] Embodiment 2 Bacillus ayerbordella CY fermentation culture fluid

[0040] (1) Incline cultivation

[0041] Inoculate Bacillus ayerbordella CY into the slant medium and culture at 30°C for 3 days to obtain slant bacteria; the composition of the slant medium is: NH 4 Cl 540mg·L -1 , K 2 HPO 4 1000mg·L -1 , KH 2 PO 4 1000 mg·L -1 , NaCl500mg·L -1 , yeast extract powder 1000mg·L -1 , acrylic acid 100mg·L -1 , MgSO 4 ·7H 2 O 4mg·L -1 , CaCl 2 1mg·L -1 , CuSO 4 1mg·L -1 , FeSO 4 1mg·L -1 , MnSO 4 1mg·L -1 , solvent is water, pH value 7.0, agar 18g L -1 .

[0042] (2) Seed culture: pick colonies from the slant of the bacteria and inoculate them into the seed medium, and cultivate them at 30°C for 18 hours to obtain the seed liquid; the final concentration of the seed medium is composed of: NaCl 10g L -1 , yeast extract powder 5g·L -1 , peptone 10 g·L -1 , the solvent is water, the pH value is 7.0.

[0043] (3) Fermentation culture: the seed liqu...

Embodiment 3

[0045] Embodiment 3: Detection of Bacillus aryabhattai CY acrylic acid degradation performance

[0046] 1. Investigate the degradation performance of acrylic acid by Bacillus ayerbordella CY under different auxiliary carbon sources

[0047] The experiment of degrading acrylic acid by Bacillus ayerbordella CY under different auxiliary carbon sources shows that the best auxiliary carbon source is yeast, and the specific implementation scheme is as follows:

[0048] Glucose, glycerol, yeast, sucrose, and sodium acetate were used as the only auxiliary carbon sources (carbon concentration 100 mg·L -1 ), according to the volume concentration of acrylic acid degradation solution 5% inoculum size will OD 600 1.5 bacteria-containing suspension (prepared by the method in Example 2) was respectively inoculated in acrylic acid liquid selective medium A (auxiliary carbon source is glucose), acrylic acid liquid selective medium B (auxiliary carbon source is glycerol), acrylic acid liquid s...

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Abstract

The invention discloses a strain of Bacillus ayerbodoides and its application in the degradation of acrylic acid, which belongs to the technical field of biological treatment of environmental pollutants. The Bacillus ayerbodoides is named CY and is preserved in the China Typical Culture Collection Center. Number: CCTCC NO: M 2019526, date of deposit is July 5, 2019. The Bacillus aeruginosa CY provided by the present invention can be 100mg·L to the initial concentration within 2d ‑1 The degradation rate of acrylic acid reached 99.8%. The discovery of the degrading bacteria is of great significance for the efficient purification of industrial wastewater containing acrylic acid.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a strain of Bacillus aryabhattai CY and its application in the degradation of acrylic acid. Background technique [0002] Acrylic acid is an important chemical raw material, widely used in paint, coating, leather, textile, printing and other industries. At present, the total annual output of acrylic acid in my country is about 1.2 million tons. According to the calculation that every ton of acrylic acid produced will produce 1.2 tons of waste water, my country will produce about 1.4 million tons of acrylic acid waste water every year. Therefore, the efficient treatment of acrylic acid wastewater has become an important content in the field of industrial wastewater pollution control. [0003] In recent years, a lot of research and application have been done on the treatment of acrylic acid wastewater at home and abroad. Cataly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F103/36C02F101/34
CPCC02F3/34C02F2101/34C02F2103/36C12N1/205C12R2001/07
Inventor 陈浚陈翌姚佳超梅瑜潘华
Owner ZHEJIANG SHUREN UNIV