Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus paracasei subsp. paracasei and application thereof

A kind of paracheese, Lactobacillus technology, applied in Lactobacillus, application, bacteria and other directions, to achieve high social application value, growth inhibition, high safety effects

Active Publication Date: 2020-03-13
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactobacillus paracasei subsp. Lactobacillus paracasei subsp. paracasei ) belongs to a subspecies of the genus Lactobacillus paracasei. So far, there is no relevant research in the industry that Lactobacillus paracasei subspecies has been developed to inhibit specific pathogenic bacteria Vibrio vulnificus. There are no relevant reports on the use of Lactobacillus paracasei subsp. paracasei for degrading azo-type artificial food colorings

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus paracasei subsp. paracasei and application thereof
  • Lactobacillus paracasei subsp. paracasei and application thereof
  • Lactobacillus paracasei subsp. paracasei and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 Lactobacillus paracasei subspecies ( Lactobacillus paracasei subsp. paracasei ) Isolation and identification of BB4

[0016] 1. Strain isolation

[0017] Obtain breast milk from a healthy woman in China, place the obtained fresh colostrum in an ultra-clean workbench and dilute it with sterile PBS solution (10 mM concentration), and divide the undiluted milk and the 5-fold diluted milk separately Put it into the BBL plate medium, and cultivate it under constant temperature and anaerobic conditions at 37°C until colonies appear. Use an inoculation needle to pick up the bacteria that grow well at the edge, and inoculate them on a new BBL plate medium. After the newly inoculated bacteria grow into colonies , and then picked the endophytic bacteria at the edge to streak culture, so that the purification was repeated until a single colony was obtained, which was named strain BB4.

[0018] Among them, the formula of BBL plate medium is: peptone 15.0 g / L, yeas...

Embodiment 2

[0024]Embodiment 2 Lactobacillus paracasei subspecies ( Lactobacillus paracasei subsp. paracasei ) Utilization of sugar by BB4

[0025] Application of API50CHL kit (Biomerieux, France) to detect the utilization of sugars by the bacterial strain BB4 isolated in Example 1, the results (Table 1) show that: Lactobacillus paracasei subspecies BB4 of the present invention can utilize ribose, D-semi Lactose, D-glucose, D-fructose, D-mannose, dulcitol, mannitol, sorbitol, N-acetylglucosamine, amygdalin, arbutin, escin iron citrate, salicin ( Salicyl alcohol), D-cellobiose, D-maltose, D-lactose, D-mebose, D-sucrose, trehalose, inulin, D-melezitose, D-raffinose, D-gentian Disaccharides, D-tagatose, D-tulonose and gluconate.

[0026] Table 1 Application of API50CHL kit to detect the utilization of sugars by strain BB4

[0027]

[0028] Remarks: + indicates a positive result, indicating that the strain can utilize the tested substrate; - indicates a negative result, indicating th...

Embodiment 3

[0029] Embodiment 3 Lactobacillus paracasei subspecies ( Lactobacillus paracasei subsp. paracasei ) Enzymatic activity of BB4

[0030] Application APIZYM kit (Biomerieux, France) detects the enzymatic activity of the isolated bacterial strain BB4 in embodiment 1, and result (table 2) shows: Lactobacillus paracasei subspecies BB4 of the present invention can secrete leucine arylase, valerine Cystine aromatase, cystine aromatase, naphthol-AS-BI-phosphohydrolase, β-galactosidase, α-glucosidase, acid phosphatase, esterase and lipase-like enzymes.

[0031] Table 2 The results of the enzyme activity of BB4 detected by APIZYM kit

[0032]

[0033] Remarks: A score of 0-1 indicates a negative reaction; a score of 2 indicates a weak reaction; a score of 3-5 indicates a positive reaction; the darker the color, the higher the score.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Viable countaaaaaaaaaa
Login to View More

Abstract

The invention discloses Lactobacillus paracasei subsp. paracasei. The strain provided by the invention has a preservation name of Lactobacillus paracasei subsp. paracasei BB4, is preserved in China General Microbiological Culture Collection Center on September 10, 2018, and has a preservation number of CGMCC No. 16455. The invention also discloses an application of the Lactobacillus paracasei subsp. paracasei in preparation of vibrio vulnificus inhibition drugs, health-care foods and common foods, and an application of the Lactobacillus paracasei subsp. paracasei in degradation of azo artificially-synthesized pigments. The Lactobacillus paracasei subsp. paracasei BB4 and fermented yoghourt prepared from the Lactobacillus paracasei subsp. paracasei BB4 provided by the invention can significantly inhibit growth of pathogenic bacteria, namely vibrio vulnificus; and the strain can significantly degrade azo artificially-synthesized food colorants. Thus, the Lactobacillus paracasei subsp. paracasei BB4 provided by the invention has high social application value.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to a strain of Lactobacillus paracasei subsp. paracasei and applications thereof. Background technique [0002] Azo-based synthetic pigments are widely used in the fields of food, medicine, and cosmetics due to their advantages such as simple synthesis process, low cost, and outstanding dyeing performance. There are many types, and the well-known ones are lemon yellow, sunset yellow, lemon yellow, amaranth, carmine and allura red. At present, many studies have found the toxic effects of azo pigments, such as abnormal growth of liver cells, affecting the expression of esterase isoenzymes, and mutagenic effects. Therefore, most azo pigments have been banned from being added to food. . According to the latest "Usage Standards for Food Additives (GB2760-2014)" promulgated by my country in 2014, 11 kinds of azo pigments such as tartrazine, sunset yell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A61K35/747A61P31/04A23L33/135A23L5/20C12R1/225
CPCA61K35/747A61P31/04A23L33/135A23L5/28A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/30
Inventor 黄菊青林斌郑怡王琦徐庆贤钱蕾官雪芳张臣心
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com