Lactobacillus paracasei subsp. paracasei and application thereof

A kind of paracheese, Lactobacillus technology, applied in Lactobacillus, application, bacteria and other directions, to achieve high social application value, growth inhibition, high safety effects

Active Publication Date: 2020-03-13
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lactobacillus paracasei subsp. Lactobacillus paracasei subsp. paracasei ) belongs to a subspecies of the genus Lactobacillus paracasei. So far, there is no relevant research in the industry that Lactobacillus paracasei

Method used

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  • Lactobacillus paracasei subsp. paracasei and application thereof
  • Lactobacillus paracasei subsp. paracasei and application thereof
  • Lactobacillus paracasei subsp. paracasei and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0015] Example 1 Lactobacillus paracasei subsp. paracasei ( Lactobacillus paracasei subsp. paracasei ) Isolation and identification of BB4

[0016] 1. Bacteria isolation

[0017] Breast milk was obtained from a healthy woman from China, and the obtained fresh colostrum was diluted with sterile PBS solution (10 mM) in an ultra-clean bench, and the undiluted milk and the 5-fold diluted milk were dispensed separately Into BBL plate medium, incubate anaerobic at 37°C until colonies appear, use an inoculation needle to pick out bacteria with good growth on the edge, and inoculate them on a new BBL plate medium. After the newly inoculated bacteria grow into colonies , and then pick the edge of the endophyte streak culture, so repeatedly purified, until a single colony was obtained, named strain BB4.

[0018] The formula of BBL plate medium is: peptone 15.0 g / L, yeast powder 2.0 g / L, glucose 20.0 g / L, soluble starch 0.5 g / L, NaCl 5.0 g / L, cysteine ​​10.0 mL / L, tomato Leachate 4...

Example Embodiment

[0024]Example 2 Lactobacillus paracasei subsp. paracasei ( Lactobacillus paracasei subsp. paracasei ) Availability of carbohydrates by BB4

[0025] The utilization of saccharides by the strain BB4 isolated in Example 1 was detected by using the API50CHL kit (Biomerieux, France). The results (Table 1) showed that: the Lactobacillus paracasei subsp. paracasei BB4 of the present invention can utilize ribose, D-half Lactose, D-glucose, D-fructose, D-mannose, dulcitol, mannitol, sorbitol, N-acetylglucosamine, amygdalin, arbutin, esculin, ferric citrate, salicin ( salicylic alcohol), D-cellobiose, D-maltose, D-lactose, D-melobiose, D-sucrose, trehalose, inulin, D-melanose, D-raffinose, D-gentian Disaccharides, D-tagatose, D-Toulonose and gluconate.

[0026] Table 1 Application of API50CHL kit to detect the utilization of saccharides by strain BB4

[0027]

[0028] Remarks: + indicates a positive result, indicating that the strain can utilize the tested substrate; - indicate...

Example Embodiment

[0029] Example 3 Lactobacillus paracasei subsp. paracasei ( Lactobacillus paracasei subsp. paracasei ) enzymatic activity of BB4

[0030] The enzyme activity of the strain BB4 isolated in Example 1 was detected by using the APIZYM kit (Biomerieux, France). The results (Table 2) showed that: Lactobacillus paracasei subsp. Amino arylamidase, cystine arylamidase, naphthol-AS-BI-phosphohydrolase, beta-galactosidase, alpha-glucosidase, acid phosphatase, esterase and lipase-like enzymes.

[0031] Table 2 Application of APIZYM kit to detect the enzymatic activity of BB4

[0032]

[0033] Remarks: A score of 0-1 indicates a negative reaction; a score of 2 indicates a weak reaction; a score of 3-5 indicates a positive reaction; the darker the color, the higher the score.

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Abstract

The invention discloses Lactobacillus paracasei subsp. paracasei. The strain provided by the invention has a preservation name of Lactobacillus paracasei subsp. paracasei BB4, is preserved in China General Microbiological Culture Collection Center on September 10, 2018, and has a preservation number of CGMCC No. 16455. The invention also discloses an application of the Lactobacillus paracasei subsp. paracasei in preparation of vibrio vulnificus inhibition drugs, health-care foods and common foods, and an application of the Lactobacillus paracasei subsp. paracasei in degradation of azo artificially-synthesized pigments. The Lactobacillus paracasei subsp. paracasei BB4 and fermented yoghourt prepared from the Lactobacillus paracasei subsp. paracasei BB4 provided by the invention can significantly inhibit growth of pathogenic bacteria, namely vibrio vulnificus; and the strain can significantly degrade azo artificially-synthesized food colorants. Thus, the Lactobacillus paracasei subsp. paracasei BB4 provided by the invention has high social application value.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applications thereof, in particular to a strain of Lactobacillus paracasei subsp. paracasei and applications thereof. Background technique [0002] Azo-based synthetic pigments are widely used in the fields of food, medicine, and cosmetics due to their advantages such as simple synthesis process, low cost, and outstanding dyeing performance. There are many types, and the well-known ones are lemon yellow, sunset yellow, lemon yellow, amaranth, carmine and allura red. At present, many studies have found the toxic effects of azo pigments, such as abnormal growth of liver cells, affecting the expression of esterase isoenzymes, and mutagenic effects. Therefore, most azo pigments have been banned from being added to food. . According to the latest "Usage Standards for Food Additives (GB2760-2014)" promulgated by my country in 2014, 11 kinds of azo pigments such as tartrazine, sunset yell...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P31/04A23L33/135A23L5/20C12R1/225
CPCA61K35/747A61P31/04A23L33/135A23L5/28A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/30
Inventor 黄菊青林斌郑怡王琦徐庆贤钱蕾官雪芳张臣心
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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