Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of Bacillus subtilis bacterial strain and its application

A Bacillus subtilis and strain technology, applied in the field of microbiology, can solve the problems of limited number of reports and few Bacillus paralicheniformis, and achieve the effects of obvious bacteriostatic effect, abundant metabolites, and metabolites and diversity.

Active Publication Date: 2020-07-17
HANGZHOU BIOCOM BIOLOGICAL TECH
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are relatively few studies on Bacillus paralicheniformis at home and abroad, and the number of relevant reports is limited

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of Bacillus subtilis bacterial strain and its application
  • A kind of Bacillus subtilis bacterial strain and its application
  • A kind of Bacillus subtilis bacterial strain and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Hangzhou Baoankang Biotechnology Co., Ltd. screened out a subtilis Bacillus paralicheniformis CGMCC №.17375, which is a new strain.

[0055] The fermentation process is as follows:

[0056] (1) Primary slant culture of Bacillus paralicheniformis CGMCC №.17375

[0057] The original strain obtained by screening is inoculated on the inclined surface of the first-level test tube, and cultivated at 37 ° C for 2-4 days. The medium formula is: beef extract 0.5-1%, peptone 1-2%, sodium chloride 0.5-1%, agar Powder 1.2-1.6%, adjust pH to 7.0-7.2.

[0058] (2) Secondary slant culture of Bacillus paralicheniformis CGMCC No. 17375

[0059] The first-stage test tube slanted seeds obtained in step (1) were inoculated in batches onto the slanted surface of the second-stage eggplant-shaped culture flask, cultured at 37°C for 2-4 days, and placed in a refrigerator at 4°C for 24 hours. The medium formula is: beef extract 0.5-1%, peptone 1-2%, sodium chloride 0.5-1%, agar powder 1.6-1....

Embodiment 2

[0064] In vitro antibacterial activity detection was carried out to the solid fermentation product obtained in step (3) of Example 1:

[0065] method one:

[0066] The obtained fermented product was dissolved and diluted with sterile water, magnetically stirred at 160 rpm for 30 min on a magnetic stirrer, and centrifuged at 3000 rpm for 5 min. Bacillus paralicheniformis CGMCC No. 17375 solid fermented product was subjected to an in vitro antibacterial test using the Oxford cup method in the agar diffusion method. The selected detection indicator bacteria include Clostridium (Clostridium perfringens GIM1.307), Staphylococcus (Staphylococcusaureus D48, Staphylococcus citreus LC5), Listeria (Listeria monocytogenes) Special bacteria Listeria monocytogenes LM1) and Enterobacter (Escherichia coli DH5), Salmonella (Salmonella enterica subsp.enterica GIM1.114, Salmonella paratyphoid A GIM1.235), Bacillus Genus (Bacillus subtilis check 01#). The indicator bacteria used were all culti...

Embodiment 3

[0074] Animal test analysis is carried out to the solid fermentation product obtained in step (3) of Example 1:

[0075] The experiment randomly selected 100 healthy 24-week-old laying hens with similar body weight, and randomly assigned 50 each to the control group and the experimental group, and added 150 g / t Bacillus paralicheniformis CGMCC №.17375 solid to the diet of the experimental group. Fermentation products, the control group was fed normally. The whole experimental feeding cycle was 42d.

[0076] The experimental results are as follows:

[0077] (1) Adding Bacillus paralicheniformis CGMCC №.17375 solid fermentation product to the diet can deepen the color of eggshells, improve the smoothness of eggshells, enhance the strength of eggshells, and reduce the rate of broken eggs.

[0078] (2) Increase the egg production rate and reduce the feed-to-egg ratio.

[0079] Table 1 Effects of Bacillus paralicheniformis CGMCC №.17375 solid fermentation products on laying perf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a bacillus subtilis strain and application thereof. The preservation number of the Bacillus paralicheniformis strain is CGMCC No. 17375. It has a wide range of applications, including the preparation of livestock and poultry feed and antibacterial drugs. Fermentation products added to the feed have good effects on the production performance of laying hens, the growth of weaned piglets and the improvement of animal immunity.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a Bacillus subtilis strain and its application. Background technique [0002] my country is the world's largest producer and user of antibiotics, and is also the hardest hit area for antibiotic abuse and bacterial resistance. In the fields of food, medicine, livestock and poultry, crops, etc., problems such as overuse of antibiotics, excessive use of antibiotics and antibiotic residues are not uncommon. Especially in the field of animal husbandry, antibiotics have the functions of preventing and treating diseases, promoting growth, and saving nutrition. Farmers generally use antibiotics in preventive medicine, disease treatment, feed addition and other links. At present, about half of the antibiotics in my country are commonly used as feed additives in the livestock and poultry breeding industry every year. Due to the imperfect supervision system, driven by interests and other reaso...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/16A23K10/18A23K50/75A23K50/30A23K50/60A61K35/742A61P31/00C12R1/125
CPCA61K35/742A23K10/16A23K10/18A23K50/30A23K50/60A23K50/75A61P31/00C12N1/20C12N1/205C12R2001/125
Inventor 王云龙吴勃徐永雷王天珍王云祥
Owner HANGZHOU BIOCOM BIOLOGICAL TECH