Method for producing protein peptide through mixed enzymolysis of poultry feather and blood

A technology of mixed enzymatic hydrolysis and poultry feathers, which is applied in the field of protein peptide production, can solve the problems of lower digestibility and palatability, easy moisture absorption of products, imbalance of amino acids, etc., and achieve the goals of improving digestibility, difficulty in preservation, and improvement of moisture absorption Effect

Pending Publication Date: 2020-03-27
秦皇岛益尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-pressure heating hydrolysis and acid-base hydrolysis can open the disulfide bonds in keratin, the reaction process will cause the loss of many amino acids (cysteine, lysine), reduce digestibility and palatability; There are problems such as time-consuming, cumbersome process, and difficulty in maintaining product stability. There are also many reports on the method of enzymatic hydrolysis using proteases, but not only the cost is high, but also the actual application effect is not satisfactory. There is also the problem of poor palatability (such as producing Bitter amino acids); puffing method is a kind of feather meal processing technology that has been adopted in recent years, but the cutting of protein by puffing method is not directional cutting, and it is not suitable for making stable and high-end protein peptide products
Generally speaking, there are still the following problems in the utilization of poultry feathers: First, there are many processing techniques, but there is still room for improvement in the coordination and unification of product digestibility, palatability, and stability, and there is a lack of preparation of high-end feathers. secondly, there is a problem of unbalanced amino acids in a single raw material; thirdly, the products after general enzymolysis are mostly spray-dried, and the products are very easy to absorb moisture, which is not only difficult to store and transport, but also difficult to meet the needs of feed Production Process Requirements
However, a single poultry blood protein also has the problem of amino acid imbalance

Method used

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  • Method for producing protein peptide through mixed enzymolysis of poultry feather and blood
  • Method for producing protein peptide through mixed enzymolysis of poultry feather and blood
  • Method for producing protein peptide through mixed enzymolysis of poultry feather and blood

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Example 1, a method for producing protein peptides by mixed enzymatic hydrolysis using poultry feathers and blood as raw materials, the obtained product is recorded as product 1, and the steps are:

[0030] (1) Put the fresh blood collected in the livestock slaughterhouse into cold storage tanks to keep fresh; wash and dehydrate the feathers collected in the slaughterhouse to be sterilized by autoclaving on the spot.

[0031] (2) Feathers are packed into the hydrolysis tank, add water, control the substrate concentration at 10% (W / W), add sodium pyrophosphite with 1% substrate mass, control the hydrolysis pressure to 0.3Mpa, and the hydrolysis temperature at 150°C , hydrolyzed for 80 minutes, open the pressure relief valve for pressure relief and exhaust, replenish water to maintain the substrate concentration of the feather hydrolyzate;

[0032] (3) Put fresh blood into a hydrolysis tank, add water, control the substrate concentration to 10% (W / W), add sodium pyrophosphi...

Embodiment 2

[0037] Example 2, a method for producing protein peptides by mixed enzymatic hydrolysis using poultry feathers and blood as raw materials, the obtained product is recorded as product 2, and the steps are:

[0038] (1) Put the fresh blood collected in the livestock slaughterhouse into cold storage tanks to keep fresh; wash and dehydrate the feathers collected in the slaughterhouse to be sterilized by autoclaving on the spot.

[0039] (2) Feathers are packed into the hydrolysis tank, add water, control the substrate concentration at 10% (W / W), add sodium pyrophosphite with 1% substrate mass, control the hydrolysis pressure to 0.3Mpa, and the hydrolysis temperature at 150°C , hydrolyzed for 80 minutes, open the pressure relief valve for pressure relief and exhaust, replenish water to maintain the substrate concentration of the feather hydrolyzate;

[0040] (3) Put fresh blood into a hydrolysis tank, add water, control the substrate concentration to 10% (W / W), add sodium pyrophosp...

Embodiment 3

[0045] Example 3, a method for producing protein peptides by mixed enzymolysis using poultry feathers and blood as raw materials, the obtained product is recorded as product 3, and the steps are:

[0046] (1) Put the fresh blood collected in the livestock slaughterhouse into cold storage tanks to keep fresh; wash and dehydrate the feathers collected in the slaughterhouse to be sterilized by autoclaving on the spot.

[0047] (2) Feathers are packed into the hydrolysis tank, add water, control the substrate concentration at 10% (W / W), add sodium pyrophosphite with 1% substrate mass, control the hydrolysis pressure to 0.3Mpa, and the hydrolysis temperature at 150°C , hydrolyzed for 80 minutes, open the pressure relief valve for pressure relief and exhaust, replenish water to maintain the substrate concentration of the feather hydrolyzate;

[0048] (3) Put fresh blood into a hydrolysis tank, add water, control the substrate concentration to 10% (W / W), add sodium pyrophosphite with...

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PUM

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Abstract

The invention provides a method for producing protein peptide through mixing enzymolysis of poultry feather and blood as raw materials. The method is characterized by comprising the following steps ofloading feather into a hydrolysis jar, adding water, controlling the concentration of substrates, adding sodium pyrosulfite of which the mass is about 1% of the substrates, and performing hydrolysis;loading blood into the hydrolysis jar, adding water, controlling the concentration of substrates, adding the sodium pyrosulfite of which the mass is 0.5% of the substrates, and performing hydrolysis;pumping feather digest and blood digest of which the ratio on a dry basis is (2-3) to 1 into the enzymolysis jar, performing mixing, performing stirring, and performing water replenishing; and reducing the temperature to about 55 DEG C, adding sodium carbonate for adjusting PH value, adding compound protease for enzymolysis, then adding aminopeptidase, performing enzymolysis until completely 6h is achieved, putting methionine and lysine in a certain proportion to the obtained feather and blood enzymolysis liquid, raising the temperature to 80 DEG C, performing heat preservation for 30min, performing enzyme deactivation to obtain material liquid to be dried, drying the material liquid to be dried with a scraping plate, and performing crushing and granulation. The method disclosed by the invention solves a digestibility problem, a palatability problem, an amino acid imbalance problem and a product moisture absorption problem existing in processing of poultry feather powder and blood.

Description

technical field [0001] The invention relates to a method for producing protein peptides by enzymatically hydrolyzing poultry feathers and blood as raw materials. Background technique [0002] In recent years, with the rapid development of the global intensive farming industry, the shortage of feed protein resources has become more and more serious, and the utilization of some unconventional protein sources (such as feathers, poultry blood, etc.) has gradually attracted widespread attention. According to the statistics of the China Animal Husbandry and Veterinary Yearbook, my country’s slaughtered poultry reached 11.99 billion in 2015. Based on the production of 0.1kg of feathers and 0.1kg of fresh blood per bird, the annual production of feathers and blood after slaughtering poultry can reach 1.199 million tons. The amount of resources is very considerable. Moreover, the downstream process of waste recycling in poultry slaughterhouses is not mature enough, and a large amount ...

Claims

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Application Information

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IPC IPC(8): A23J1/06A23J1/10A23J3/34A23K20/147A23K50/30A23K50/60A23K50/80
CPCA23J1/06A23J1/10A23J3/345A23J3/341A23K20/147A23K50/30A23K50/60A23K50/80Y02A40/818
Inventor 张维金
Owner 秦皇岛益尔生物科技有限公司
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