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Lactobacillus plantarum effect of preventing and treating decayed teeth

A Lactobacillus plantarum, dental caries technology, applied in the field of microorganisms, can solve the problems of weakening, inability to resist plaque, tooth pigmentation and other problems

Pending Publication Date: 2020-04-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional brushing and cleaning methods cannot fight stubborn plaque, and children are often limited by their will
When using ordinary Chinese herbal medicine products for prevention, although it may inhibit the cariogenic flora, it will also weaken other dominant flora that maintain oral balance. The use of Chinese herbal medicine products will also cause the risk of tooth pigmentation

Method used

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  • Lactobacillus plantarum effect of preventing and treating decayed teeth
  • Lactobacillus plantarum effect of preventing and treating decayed teeth
  • Lactobacillus plantarum effect of preventing and treating decayed teeth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Animal experiments on the use of Lactobacillus plantarum CCFM8724 in the prevention and treatment of dental caries

[0044] Experimental animals and strains:

[0045] SPF-grade Wistar rats, male, 3 weeks old, weighing 40-50 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (production license number SCXK (Beijing) 2012-0001).

[0046] Streptococcus mutans was Streptococcus mutans ATCC 25175, which was purchased from CGMCC, China General Microorganism Collection Center. Candida albicans was isolated from the oral cavity of volunteers.

[0047] Lactobacillus plantarum CCFM634 (CGMCC No.9740), as another strain of Lactobacillus plantarum with a better effect of inhibiting bibacterial biofilm in vitro (24h-mediated inhibition of biofilm amount> 60%), was also used in animal experiments for comparison. For the experiment of inhibiting the biofilm of double bacteria, the mediation group was to add 50 μL of Streptococcus mutans a...

Embodiment 2

[0088] Embodiment 2 prepares the bacteria powder containing Lactobacillus plantarum CCFM8724 live bacteria

[0089] Lactobacillus plantarum CCFM8724 was added to the MRS liquid fermenter with 10% inoculum and cultivated for 12 hours, the pH of the fermentation process was controlled to 5.4-5.8, the fermentation was terminated and the temperature was lowered to below 20°C, and the bacteria were collected by centrifugal equipment. Add lyoprotectant (4% glycerin, 25% skimmed milk powder, 4% ascorbic acid, 1.5% sodium carbonate) at 1:1 ratio, and directly add the protective agent to the bacteria sludge to balance the emulsification, and the emulsification time is 30 minutes. The emulsion added with the bacteria slime was placed in a freeze dryer at -40°C and 4Pa for 48 hours, and the freeze-dried product was subjected to low-intensity crushing and sieving to obtain a high-activity probiotic freeze-dried powder.

Embodiment 3

[0090] Embodiment 3 prepares the yogurt containing Lactobacillus plantarum CCFM8724 live bacteria

[0091] Milk powder, inulin, stevia, and water are mixed according to the weight ratio: 20:5:5:75, homogenized, and made into fermentation raw materials; sterilized by ultra-high temperature sterilization at 121°C for 300s, then cooled to 42°C, inoculated and fermented Bacteria Lactobacillus bulgaricus and Streptococcus thermophilus mixed bacteria powder, the inoculation amount of fermentation bacteria is 0.3% of the total weight of fermentation raw materials; after 12 hours of fermentation at 42°C, it is prepared; the fermentation product is cooled to 37°C, and Lactobacillus plantarum CCFM8724 is added Freeze-dried bacteria powder, the feeding amount is 10 9 CFU / ml yogurt, stirred, canned, stored at 4°C for 2 days and naturally cooked to make probiotic yogurt.

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Abstract

The invention discloses lactobacillus plantarum with an effect of preventing and treating decayed teeth, which belongs to the technical field of microorganisms. According to the invention, a rat decayed tooth model is established; the serum biochemical indexes, oral flora, tooth demineralization and decayed tooth conditions of mice in a blank control group, a model group, an intervention group anda prevention group are compared, the lactobacillus plantarum is determined that the lactobacillus plantarum CCFM8724 has the effect of preventing and treating decayed teeth, the application effect ofthe lactobacillus plantarum CCFM8724 is superior to that of a traditional anti-caries drug chlorhexidine, and the lactobacillus plantarum CCFM8724 has broad market application prospects. Meanwhile, the lactobacillus plantarum CCFM8724 can be applied to probiotic functional foods and daily chemical products such as bacterial powder, yoghourt, chewable tablets, chewing gum, toothpaste and mouthwash, and is expected to open a new functional market for preventing and treating decayed teeth by probiotics.

Description

technical field [0001] The invention relates to a kind of probiotics for preventing and treating dental caries caused by the infection of Streptococcus mutans (hereinafter referred to as variable chain) and Candida albicans (hereinafter referred to as Candida albicans), especially a strain of Lactobacillus plantarum with the effect of preventing dental caries, which belongs to the technical field of microorganisms . Background technique [0002] Caries is a bacterial infectious disease that occurs on enamel and dentin, and the formation of dental plaque biofilm is a prerequisite for the occurrence of caries. Mature plaque biofilm is a three-dimensional ecological environment formed by oral bacteria embedded in an insoluble polysaccharide-based matrix, rich in voids and piping systems, and has a certain thickness. Once the ecological imbalance is caused by changes in the host, diet, microbial growth and other factors, the cariogenic microorganisms in the dental plaque biofil...

Claims

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Application Information

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IPC IPC(8): A61K35/747A61P1/02A61P31/04A61P31/10A23C9/123A23C9/13A23G4/12A23K10/18A23L33/135
CPCA61K35/747A61P1/02A61P31/04A61P31/10A23C9/1238A23C9/1307A23G4/123A23K10/18A23L33/135A23V2400/123A23V2400/249A23V2400/169
Inventor 陈卫张秋香秦苏佳张灏赵建新
Owner JIANGNAN UNIV
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