Curcumin double-layer emulsion with colon-targeted transit function and production method and application of curcumin double-layer emulsion with colon-targeted transit function

A technology of colon targeting and curcumin, which is applied in the direction of emulsion delivery, medical preparations and applications of non-active ingredients, can solve the problems of increasing curcumin bioavailability and curcumin degradation, and achieve good biocompatibility, Avoid adverse effects and enhance the effect of bioavailability

Active Publication Date: 2020-05-05
QINGDAO AGRI UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, carboxymethyl konjac glucomannan is mostly used to prepare microcapsules and microspheres to treat sewage or make plasma substitutes. There are no reports or patents on the use of carboxymethyl konjac glucomannan for the preparation of curcumin Emulsion Delivery System to Increase Curcumin Bioavailability and Slow Curcumin Degradation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Curcumin double-layer emulsion with colon-targeted transit function and production method and application of curcumin double-layer emulsion with colon-targeted transit function
  • Curcumin double-layer emulsion with colon-targeted transit function and production method and application of curcumin double-layer emulsion with colon-targeted transit function
  • Curcumin double-layer emulsion with colon-targeted transit function and production method and application of curcumin double-layer emulsion with colon-targeted transit function

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0041] 1. Preparation of materials and solutions

[0042] 1. Preparation of carboxymethyl konjac glucomannan (CMKGM): Weigh 5g konjac glucomannan and dissolve in 40mL 70% (v / v) ethanol, add 40% (w / v) NaOH solution, dissolve Afterwards, it was stirred in a water bath at 50°C for 1 h for alkalization treatment. After alkalization and swelling, according to the chloroacetic acid (MCA):NaOH molar ratio of 1:1, 1.5:1, 2:1, 2.5:1, 3:1, add the aqueous solution of chloroacetic acid, react at 50°C for 2 hours, and use 1.0mol / Adjust the pH of the solution to 7 with L HCl, collect the precipitate, wash the precipitate several times with 70% (v / v) ethanol, 95% (v / v) ethanol and absolute ethanol, and dry it with hot air at 50°C until constant weight. CMKGM with a degree of substitution of 0.28~0.74 and a potential of -26.9mV~-38.6mV was obtained.

[0043]2. Preparation of whey protein isolate solution: Accurately weigh 0.5~2.5g whey protein isolate powder, dissolve in 50mL deionized wa...

Embodiment 1

[0076] 1, the preparation process flow chart of curcumin double-layer emulsion of the present invention is shown in Figure 12 , in the present embodiment, the preparation method of curcumin bilayer emulsion comprises the following steps:

[0077] (1) Mix 10 mg / mL curcumin oil phase with 2.0% (w / v) whey protein isolate solution at a volume ratio of 1:6, adjust the pH of the solution to 7, and use a homogeneous emulsifier at 25°C Homogenize at 11000r / min for 3min to prepare curcumin O / W primary emulsion.

[0078] (2) Mix the curcumin O / W primary emulsion in step 1 with 0.4% (w / v) carboxymethyl konjac glucomannan solution according to the volume ratio of 1:1, and the carboxymethyl The degree of substitution of konjac glucomannan is 0.74, the potential is -38.6mV, the pH of the mixed solution is adjusted to 3.8, and a magnetic stirrer is used to stir at 300r / min for 30min to obtain a curcumin double layer emulsion.

[0079] 2. Experimental results

[0080] Detect the macroscop...

Embodiment 2

[0085] In the present embodiment, the preparation method of curcumin double-layer emulsion comprises the following steps:

[0086] 1. Mix 10 mg / mL curcumin oil phase with 0.5% (w / v) whey protein isolate solution at a volume ratio of 1:9, adjust the pH of the solution to 6.5, and use a homogeneous emulsifier at 25°C at 13,000 r / min homogenization for 5 minutes, and the curcumin O / W primary emulsion was prepared.

[0087] 2. Mix the curcumin O / W primary emulsion in step 1 with 0.1% (w / v) carboxymethyl konjac glucomannan solution according to the volume ratio of 1:3, and then carboxymethyl konjac The degree of substitution of glucomannan was 0.28, the potential was -26.9mV, the pH of the mixed solution was adjusted to 4, and a magnetic stirrer was used to stir at 500 r / min for 30 minutes to obtain a curcumin double-layer emulsion.

[0088] The curcumin double-layer emulsion prepared under this condition has a drug loading of 0.25 mg / g, is placed in simulated gastric juice and si...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a curcumin double-layer emulsion with a colon-targeted transit function and a production method and application of the curcumin double-layer emulsion with the colon-targeted transit function. According to the curcumin double-layer emulsion with the colon-targeted transit function and the production method and application of the curcumin double-layer emulsion with the colon-targeted transit function, a whey protein isolate solution is adopted as an emulsifier, carboxymethyl konjac glucomannan is adopted as an outer-layer polyelectrolyte, and by means of a static layer-by-layer self-assembly technology, the curcumin double-layer emulsion is produced. The production method is simple in process, by changing a system pH value, a ratio of protein to a polysaccharide, the dosage and the like, properties of the emulsion can be controlled, and the stability of the emulsion is improved. According to the produced curcumin double-layer emulsion, not only are the advantages of emulsions, which are produced by using protein and a polysaccharide as interface layers respectively, combined, but also adverse effects, caused by environments, on curcumin can be avoided; and therelease amount in simulated gastric fluid is low, while the release amount in simulated colon fluid containing beta-mannase is high, thus the produced curcumin double-layer emulsion has excellent colon-targeted transit performance, the bioavailability of the curcumin is greatly improved, and application ranges of the curcumin in the fields of food, medicines and the like are effectively extended.

Description

technical field [0001] The invention belongs to the technical field of targeted release, and in particular relates to a curcumin double-layer emulsion with colon targeted delivery function, a preparation method and application thereof. Background technique [0002] Curcumin is the active ingredient of the perennial herb turmeric. It not only has various functions such as anti-oxidation, anti-inflammation, anti-cancer, promotion of wound healing and prevention of neurodegenerative diseases, but also has a variety of physiological effects in molecular biology, such as inhibiting Colon cancer cell cycle progression, reduction of angiogenesis and induction of apoptosis, etc. Due to the strong intermolecular and intramolecular hydrogen bonds of curcumin, the solubility and dissolution rate of curcumin in water are extremely low, which makes the compatibility of curcumin with other food raw materials extremely poor during food processing, and the in vivo The bioavailability of cu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K31/12A61K47/36A61K47/42A61P17/02A61P25/00A61P29/00A61P35/00A61P39/06A23L29/10A23L29/30A23L33/105
CPCA23V2002/00A61K9/107A61K31/12A61K47/36A61K47/42A23L29/10A23L29/30A23L33/105A61P17/02A61P25/00A61P29/00A61P35/00A61P39/06A23V2250/2112A23V2250/54252A23V2200/222A23V2250/5058
Inventor 肖军霞王鲁慧黄国清张馨如
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap