Human serum albumin magnetic bionic immunoassay kit

Abstract

The invention discloses a human serum albumin magnetic bionic immunoassay kit. The kit comprises an HSA magnetic molecularly imprinted polymer, HRP-HSA and an HSA standard substance. The HSA magneticmolecularly imprinted polymer is a bionic antibody which is synthesized by adopting a sol-gel surface polymerization method and has a recognition function on HSA. The preparation method specifically comprises the following steps: preparing magnetic nano-microspheres, modifying the magnetic nano-microspheres with amino groups, forming a molecularly imprinted polymer layer through sol-gel polymerization, removing template molecules HSA combined on the molecularly imprinted polymer layer and other steps. The kit can be used for analyzing and determining the content of human serum albumin in urineand has the advantages of low cost, convenience in operation, good stability and high accuracy.

Description

technical field [0001] The invention belongs to the field of immune analysis and detection, and in particular relates to a kit for analyzing the content of human serum albumin by using an artificial antibody of human serum albumin magnetic molecular imprinting polymer. Background technique [0002] Human serum albumin (Human Serum Albumin, HSA) is the most abundant protein in human plasma, it can bind and transport endogenous and exogenous substances, has the functions of maintaining blood osmotic pressure, scavenging free radicals, inhibiting platelet aggregation and anti- Physiological functions such as blood coagulation play an important role in life activities. Albumin is a normal protein in the blood. Under physiological conditions, only a small amount of albumin is excreted in the urine. When the kidney metabolism is abnormal, the albumin content in the urine will increase. Therefore, the detection of trace albumin content in urine is commonly used. Early kidney disea...

AI Technical Summary

Problems solved by technology

Chinese patent "Preparation and Application of Targeted Enzyme Immobilization Carrier Based on Magnetic Molecular Imprinting Technology" (CN110240704A) also uses 3-aminopropyltriethoxysilane as the functional monomer and orthosilicic acid in the aqueous phase system Ethyl ester is a cross-linking monomer, which is prepared by sol-gel method to obtain Fe coated with boronic acid modification. 3 o 4 Molecularly imprinted polymer layers on magnetic nanoparticles, but the patent was not used to make biomimetic antibodies

Although the polymers formed by silane hydrolysis and polymerization have better properties, most silane reagents have poor water solubility and only have good solubility in organic solvents. Since proteins will be denatured in organic solvents, it is very important for development (screening) to prepare protein molecular imprinting. Polymer silane reagent monomer puts forward higher requirements

Images