Preparation method of cornus officinalis polypeptide and cornus officinalis polypeptide prepared by method

A technology of dogwood and enzyme preparation, which is applied in the field of natural product extraction, can solve the problems of low protein utilization rate and the like, and achieve the effects of improving effective utilization and absorption efficiency, enhancing body immunity and high protein content.

Active Publication Date: 2020-05-08
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an enzyme preparation for extracting polypeptides from Cornus officinalis, the enzyme preparation can hydrolyze the protein of Cornus officinalis into polypeptides, so as to solve the problem of low protein utilization rate in cornus officinalis in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 is used for the preparation of the enzyme preparation that extracts polypeptide from Cornus officinalis

[0023] 1.1 The enzyme preparation includes compound enzyme A and compound enzyme B, wherein compound enzyme A includes 0.05 parts by weight of phytase, 1 part by weight of cellulase, and 0.1 parts by weight of pepsin; compound enzyme B includes 0.2 parts by weight of papain, 0.1 parts by weight of trypsin parts by weight, flavor protease 0.3 parts by weight.

[0024] 1.2 The enzyme preparation includes compound enzyme A and compound enzyme B, wherein compound enzyme A includes 0.2 parts by weight of phytase, 0.05 parts by weight of cellulase, and 1.5 parts by weight of pepsin; compound enzyme B includes 1 part by weight of papain, 1 part by weight of trypsin parts by weight, flavor protease 0.5 parts by weight.

[0025] 1.3 The enzyme preparation includes compound enzyme A and compound enzyme B, wherein compound enzyme A includes 0.8 parts by weight of...

Embodiment 2

[0029] (1) Wash the Cornus officinalis with clean water to remove the core, dry it, crush it, and sieve it through a 100-mesh sieve for later use;

[0030] (2) Add Cornus officinalis powder and 9% sodium chloride solution at a ratio of 1:8 (g:mL) to liquid, and adjust the pH value of the solution to 9.0 with sodium hydroxide to prepare Cornus officinalis slurry;

[0031] (3) stirring and reacting the Cornus officinalis slurry at 55° C. for 2.5 hours, and then filtering and collecting the supernatant;

[0032](4) Regulate the pH value of the supernatant to be 4.5, add food-grade chitosan, and centrifuge after the solution is clarified to obtain the crude protein of Cornus officinalis;

[0033] (5) Wash the cornus crude protein three times with deionized water, then dissolve it with deionized water, adjust the pH value of the cornus crude protein solution to 4.0, add mass concentrations of 0.5% phytase, 2.0% cellulase and 0.25% The pepsin was hydrolyzed for 2 hours at 35°C (the...

Embodiment 3

[0038] (1) Wash the Cornus officinalis with clean water to remove the core, dry it, crush it, and sieve it through a 100-mesh sieve for later use;

[0039] (2) Add Cornus officinalis powder and 7% sodium chloride solution at a ratio of 1:6 (g:mL) to liquid, and adjust the pH value of the solution to 8.0 with sodium hydroxide to prepare Cornus officinalis slurry;

[0040] (3) Stirring and reacting the Cornus officinalis slurry at 70° C. for 4 hours, then filtering and collecting the supernatant;

[0041] (4) Regulating the pH value of the supernatant to 5.0, adding food-grade chitosan, clarifying and centrifuging to obtain the cornus crude protein;

[0042] (5) Wash the crude protein of Corni officinalis three times with deionized water, then dissolve it, adjust the pH value of the crude protein solution of Corni officinalis to 5.5, add 1.0% phytase, 1.5% cellulase and 0.5% pepsin45 Hydrolysis at ℃ for 3 hours (the concentrations of above-mentioned phytase, cellulase and pepsi...

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Abstract

The invention belongs to the field of extraction of natural products, and specifically relates to a preparation method of cornus officinalis polypeptide and the cornus officinalis polypeptide preparedby the method. The cornus officinalis polypeptide of the present invention is mainly prepared by enzymatic hydrolysis of cornus officinalis protein with an enzyme preparation. The enzyme preparationfor extracting polypeptide from cornus officinalis comprises a complex enzyme A and a complex enzyme B. The complex enzyme A comprises phytase, cellulase and pepsase. The complex enzyme B comprises alkaline protease and flavourzyme. The preparation method of the cornus officinalis polypeptide provided by the invention has simple preparation process and low glycoprotein content. The prepared cornusofficinalis polypeptide has the advantages of high protein content, easy dissolution, low viscosity, high acid-base stability and easy absorption.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and in particular relates to a preparation method of cornus officinalis polypeptide and the prepared cornus officinalis polypeptide. Background technique [0002] Cornus Officinalis Sieb.et Zucc., as a kind of Chinese herbal medicine, has the effects of invigorating the stomach, nourishing the liver and kidney, curing anemia, lumbago, and weakness of the nerves and heart. The main production areas in my country are western Henan, Zhejiang and other places. Cornus officinalis is rich in nutrients and a variety of essential amino acids. It is a rare medicinal and edible plant. However, the macromolecular plant protein in Cornus officinalis is covered by cellulose, which has low solubility, high viscosity and certain antigenicity. The absorption and digestion efficiency of human body is far lower than that of animal protein, which greatly limits the development of Cornus officinalis pulp ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/76C12N9/64C12N9/50C12N9/42C12N9/16C12P21/06A23L33/18
CPCC12N9/6481C12N9/6427C12N9/63C12N9/50C12N9/2437C12N9/16C12P21/06A23L33/18C12Y304/21004C12Y301/03008C12Y301/03026C12Y302/01074C12Y304/22002A23V2002/00A23V2200/324A23V2200/30A23V2250/55Y02P60/87
Inventor 张鑫李彦松王鹏
Owner NANYANG NORMAL UNIV
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