Saponin compound or pharmaceutically acceptable salt and composition thereof as well as preparation method and application thereof
A kind of compound, saponins technology, applied in the field of natural products
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Embodiment 1
[0063] 1. Extraction and separation
[0064] Gynostemma pentaphyllum 5kg, 60% v / v ethanol aqueous solution soaking and extracting at room temperature three times, once a week, filtering, concentrating under reduced pressure to obtain the extract extract, adding appropriate amount of water to suspend, and using petroleum ether, ethyl acetate and n-butanol respectively Extract three times. The ethyl acetate extract was chromatographed on a silica gel column, and gradient eluted with a dichloromethane-methanol mixed solvent to obtain 10 fractions (fraction 1-10); wherein fraction 7 (dichloromethane:methanol volume ratio was 8:2 ) was separated and purified by reverse ODS column chromatography, using methanol: water mobile phase gradient elution (using 10% v / v methanol water, 20% v / v methanol water, 30% v / v methanol water, 60% v / v methanol aqueous solution elution), the fraction of 60% v / v methanol aqueous solution was prepared and separated by high-efficiency liquid phase metho...
Embodiment 2
[0081] 1 Materials, reagents and instruments
[0082] 1.1 Materials
[0083] Dissolve the compound of formula (I) in DMSO, prepare a stock solution with a concentration of 1mg / mL, store it in a -20°C refrigerator, and dilute to the required concentration before use.
[0084] 1.2 Reagents
[0085] DMEM (high glucose) medium (Hyclone, USA); fetal bovine serum (PBS, Hyclone, USA); double antibody (100U / mL penicillin + 10mg / mL streptomycin); Cell Counting Kit-8 kit was purchased from Dojindo company.
[0086] 1.3 Instruments
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[0089] 1.4 Reagent preparation
[0090] 1.4.1 Culture medium preparation
[0091] Take 45 mL of DMEM / High Gluose basal medium, add 5 mL of fetal bovine serum (final concentration 10%) and 500 μL double antibody solution (final concentration of penicillin 100 U / mL, final concentration of streptomycin 0.1 mg / mL), store in 4°C refrigerator for later use.
[0092] 1.4.2 Preparation of PBS buffer solution (1L)
[0093] Weigh 0.2g K...
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