High-efficiency extraction method of macadamia nut leaf total proteins

An extraction method and protein technology, applied in the field of protein extraction, can solve the problems of easy oxidative browning and low protein purity, and achieve the effect of avoiding protein degradation, wide distribution of protein size and good quality
CN111171108AActive Publication Date: 2020-05-19SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH SUBTROPICAL CROPS RES INST CHINESE ACAD OF TROPICAL AGRI SCI
Publication Date
2020-05-19

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Abstract

The invention discloses a high-efficiency extraction method of macadamia nut leaf total proteins. The method comprises the following steps: grinding and crushing fresh macadamia nut leaves by using liquid nitrogen; adding a pre-cooling cracking liquid PW1 for primary extraction; performing solid-liquid separation to obtain a first precipitate; cleaning and airing the first precipitate; adding an extracting solution PW2 for secondary extraction; performing solid-liquid separation to obtain a second supernatant; adding a pre-cooling methanol solution; performing centrifuging to obtain a second precipitate; cleaning and drying the second precipitate; adding a dissolving solution PW3 for tertiary extraction; and performing solid-liquid separation to obtain a third supernatant, namely the macadamia nut leaf total proteins. According to the invention, the fresh macadamia nut leaves are taken as materials, and the three extracting solutions are added into macadamia nut leaf powder, so that oxidation of raw materials can be effectively avoided, degradation of proteins can be avoided, the yield of the total proteins is high, purity of the extracted protein is high, the SDS-PAGE electrophoresis bands are neat and clear, and the macadamia nut leaf powder is suitable for Western Blot analysis.
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Description

technical field

[0001] The invention belongs to the technical field of protein extraction. More specifically, it relates to a high-efficiency extraction method for total protein in leaves of macadamia nuts. Background technique

[0002] Macadamia ternifolia F.Muell., also known as Queensland chestnut, macadamia nut, and macadamia walnut, belongs to the genus Macadamia of the family Longanaceae. It is an evergreen tree native to southeastern Queensland and northern New South Wales, Australia. Coastal subtropical rainforest at 25Β°-31Β° south latitude. Macadamia nuts are rich in nutrients, with an oil content of 70% to 79%, especially rich in unsaturated fatty acids, rich in protein, and rich in phosphorus, calcium, iron, vitamins, amino acids, etc., known as the "Queen of Dried Fruits" and "No. King". At present, research on macadamia at home and abroad is mainly focused on the promotion of cultivation techniques, germplasm identification, resource utilization, etc. There ar...

Claims

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