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Bacillus methylotrophicus LW-6 deodorization and epidemic prevention agent for livestock farm air treatment

A technology of methyl nutritional type, deodorant and anti-epidemic agent, applied in the field of microorganisms, can solve the problems of lack of efficient and safe biological deodorant, no effective treatment method, etc., and achieve the effects of stable microbial activity, ideal effect and good effect.

Pending Publication Date: 2020-05-26
陕西恒田生物农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is still a lack of efficient and safe biological deodorants, especially there is no effective treatment method for the odor of methyl mercaptan, and methylotrophic bacillus can just use these odors as nutrients for their own growth to absorb and transform and degrade

Method used

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  • Bacillus methylotrophicus LW-6 deodorization and epidemic prevention agent for livestock farm air treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The deposit registration number of the methylotrophic bacillus in Example 1 is: CGMCC No.6793, and the depository institution is: the methylotrophic bacillus of the General Microbiology Center of the China Committee for the Collection of Microorganisms.

[0025] Example 1:

[0026] 1 Inoculate the slant of the methylotrophic bacillus test tube on a plate of a specific medium for colony activation and amplification, inoculate the amplified colony plate in a specific liquid medium for activation and culture for 92 hours, and obtain an activated flora liquid ;

[0027] 2 The activated flora liquid is inoculated in a liquid fermentation medium with a specific formula at 8% by volume, and fermented and cultivated for 120 hours at 32° C. to obtain a methylotrophic bacillus fermentation broth.

[0028] 3 Methylotrophic bacillus fermented bacteria liquid is subjected to ultrafiltration concentration and multi-layer filtration under specific conditions to prepare a methylotroph...

Embodiment 2

[0034] Get the methylotrophic bacillus LW-6 deodorizing and anti-epidemic agent that embodiment 1 makes, mix evenly with water in the ratio of mass ratio 1:80, according to the spray amount of 15 milliliters per square meter, spray on livestock and poultry In the breeding shed, spray 3 times.

[0035] Before the chicks enter the shed, place 5 plates of different bacteria in different positions (four corners and middle positions) in the shed, cover them after 10 minutes, take them out, and then spray the deodorant and anti-epidemic agent in the shed. After 8 hours, put them into different plates in the same way, take them out after 10 minutes, put them in a constant temperature incubator at 32°C for 48 hours, and count the number of bacteria before and after disinfection. Calculate the bacteriostatic rate, the results are as follows: Staphylococcus aureus 72%, Escherichia coli 65%, Salmonella pullorum 82%, Pasteurella multocida 63%.

Embodiment 3

[0037] This example is used to investigate the deodorizing ability of the methylotrophic Bacillus LW-6 deodorant and anti-epidemic agent.

[0038]The methylotrophic bacillus LW-6 deodorizing and anti-epidemic agent prepared by the embodiments of the present invention 1 and 2 is mixed with water in a ratio of 1:80 by mass, and according to the spraying amount of 15 milliliters per square meter, Spray in the livestock and poultry breeding shed. Randomly take chicken manure from the farm, take 20 kg each, put it into three test boxes, spray methylotrophic Bacillus deodorizing and anti-epidemic agent every 12 hours, and detect ammonia after 48 hours gas, methyl mercaptan and hydrogen sulfide concentrations.

[0039] After testing, the deodorizing results of the methylotrophic bacillus deodorizing and anti-epidemic agent of this embodiment are as follows:

[0040]

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Abstract

The invention relates to a bacillus methylotrophicus LW-6 deodorization and epidemic prevention agent for livestock farm air treatment. The bacillus methylotrophicus LW-6 deodorization and epidemic prevention agent for livestock farm air treatment is used as a liquid spray for the first time to be applied to a livestock farm. The strain has low requirements on nutrition; stress resistance, growthand propagation speed, rapid growth and field planting can be realized after spraying to the environment, various antibacterial substances, various enzymes and the like are generated through metabolism; therefore, antagonism, inhibition or killing effects are generated on pathogenic bacteria; therefore, the purpose of air treatment in the breeding environment of disinfection, deodorization, purification and epidemic prevention is achieved, the agent has the characteristics of safety to people and livestock, less environmental pollution, strong pertinence to prevention and treatment objects andthe like, and has no side effect and good effect, so that the use of chemical deodorization and epidemic prevention agents which are always adopted at home and abroad at present can be reduced.

Description

technical field [0001] The invention relates to a methylotrophic bacillus LW-6 deodorizing and anti-epidemic agent used for air treatment in animal husbandry farms, belonging to the technical field of microorganisms. Background technique [0002] Worldwide, livestock and poultry epidemic diseases and common common diseases caused by pathogenic bacteria are one of the main reasons for the loss of breeding industry. Moreover, most of the livestock and poultry raised in modern large-scale are kept in closed pens, and the pathogenic bacteria contained in their excrement and secretions cause air odor and pathogen pollution, which induces frequent outbreaks of livestock and poultry mass diseases. For half a century, People mainly adopt chemical disinfectants to prevent and treat this type of disease and odor control, but the effect is very small. On the other hand, many chemical disinfectants have the characteristics of high toxicity, high residue, refractory degradation and seco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/58C12R1/07
CPCC12N1/20B01D53/84B01D53/58B01D2251/95B01D2258/0266Y02A50/20
Inventor 唐满仓安航安德荣
Owner 陕西恒田生物农业有限公司