High-efficiency cyhalothrin hapten, activated carrier protein, complete antigen and polyclonal antibody for immunoassay

Abstract

The invention relates to the technical field of detection, and discloses an efficient cyhalothrin hapten, an activated carrier protein, a complete antigen and a polyclonal antibody, which are used forimmunoassay. The low-toxicity reagent selectively hydrolyzes the cyano group, the efficient cyhalothrin hapten can be prepared by a one-step method, and the cyano group in the efficient cyhalothrin is converted into amide. The hapten with the amide group is coupled with succinic anhydride activated carrier protein to form a complete antigen; the complete antigen is used for preparing a corresponding polyclonal antibody. The lowest detection limit of the antibody on efficient cyhalothrin is 10 micrograms per liter. The antibody has no obvious cross reaction on other pyrethroid pesticides (deltamethrin, cypermethrin, cyfluthrin and fenvalerate).

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a high-efficacy-cyhalothrin hapten, an activated carrier protein, a complete antigen and a polyclonal antibody for immune analysis. Background technique [0002] Pyrethroid insecticides are a class of synthetic insecticides that mimic natural pyrethrins and are insect neurotoxins biosynthesized from pyrethrins. So far, pyrethroids have occupied more than 18% of the global pesticide market. Lambda-cyhalothrin (as shown in formula 1 below) is one of the important components of this type of insecticide, and is widely used in agricultural production. Although its toxicity is widely believed to be low, the results of two recent studies have raised major questions about the molecule's safety in humans. For example, studies have shown that overexposure to cypropathrin may result in myalgia, acute respiratory irritant injury, and shock. On the other hand, since residues of lambda-cy...

AI Technical Summary

Problems solved by technology

In the hapten synthesis of lambda-cyhalothrin, although a variety of synthetic methods have been published, there is still room for optimization of their synthetic routes (as shown below)

For example, Gao et al. reported the multi-step synthesis of pyrethroid haptens, but this method has highly toxic reagents (some other pyrethroid hapten synthesis methods also have this problem, such as the method reported by Lee et al. in c), and the total Problem with low rate (10.4%)

Luo and colleagues reported the use of non-toxic KMnO 4 Oxidative one-step synthesis of the lambda-cyhalothrin hapten, but this method removes the key functional group (-CF 3 and -Cl), the structure of the resulting hapten is too general and thus considered nonspecific

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