High-efficiency cyhalothrin hapten, activated carrier protein, complete antigen and polyclonal antibody for immunoassay

A high-efficiency cyhalothrin and immunoassay technology, applied in the direction of immunoglobulin, immunoglobulin from serum, ovalbumin, etc., can solve the problems of non-specificity, low total yield, high toxicity, etc., and achieve strong specificity The effect of stability, easy industrial production, and high structural fidelity

Active Publication Date: 2020-06-12
OCEAN UNIV OF CHINA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the hapten synthesis of lambda-cyhalothrin, although a variety of synthetic methods have been published, there is still room for optimization of their synthetic routes (as shown below)
For example, Gao et al. reported the multi-step synthesis of pyrethroid haptens, but this method has highly toxic reagents (some other pyrethroid hapten synthesis methods also have this

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-efficiency cyhalothrin hapten, activated carrier protein, complete antigen and polyclonal antibody for immunoassay
  • High-efficiency cyhalothrin hapten, activated carrier protein, complete antigen and polyclonal antibody for immunoassay
  • High-efficiency cyhalothrin hapten, activated carrier protein, complete antigen and polyclonal antibody for immunoassay

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] The raw material is in mmol, and the one-step synthesis method specifically includes the following steps: 1.5-2.5 mmol of efficient-cyhalothrin is packed into a reaction vessel and cooled in an ice-water bath, and then 3.5-4.5 mmol of trimethyl ((trifluoromethyl )sulfonyl)silane, stirred for 1-3min until uniform; after lambda-cyhalothrin is completely dissolved, slowly add 70-80μL of water within 1.5-2.5min and stir the resulting mixture for 3-5h; then use saturated NaHCO 3 solution to neutralize the reaction, the organic reagent CH 2 Cl 2 Extract multiple times, each time with an amount of 10-20mL; mix the organic phases and use excess Na 2 SO 4 Dry and concentrate in vacuo; the product is separated using a silica gel column, and the eluent is a mixture of ethyl acetate and n-hexane with a volume ratio of (35-65):(45-55).

[0051] The preparation method of lambda-cyhalothrin complete antigen comprises the following steps:

[0052] 1) carrier protein activation: th...

specific Embodiment

[0057] Material

[0058] Reagents: silica gel (125-150 μm) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China); lambda-cyhalothrin was purchased from Wuhan Yuancheng Gongchao Technology Co., Ltd. (Wuhan, China); Deltamethrin, cypermethrin, cyfluthrin, and esfenvalerate were all obtained from the National Research Center for Standard Materials of China (Beijing, China). Skim milk powder was purchased from Reanal (Hungary). Chemicals for Hapten Synthesis and Conjugation to Carrier Proteins, Horseradish Peroxidase (HRP), Bovine Serum Albumin (BSA), Ovalbumin (OVA), and Tetramethylbenzidine (TMB) Conjugated Goat Anti-mouse IgG was purchased from Sigma-Aldrich Chemical Co. (Milwaukee, USA). α-cyano-4 hydroxycinnamic-acid (HCCA), trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich Chemical Co., Ltd. (Milwaukee, USA).

[0059] Apparatus: Enzyme-linked immunosorbent assays (ELISAs) were performed in 96-well microplates (No. 3655) purchase...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of detection, and discloses an efficient cyhalothrin hapten, an activated carrier protein, a complete antigen and a polyclonal antibody, which are used forimmunoassay. The low-toxicity reagent selectively hydrolyzes the cyano group, the efficient cyhalothrin hapten can be prepared by a one-step method, and the cyano group in the efficient cyhalothrin is converted into amide. The hapten with the amide group is coupled with succinic anhydride activated carrier protein to form a complete antigen; the complete antigen is used for preparing a corresponding polyclonal antibody. The lowest detection limit of the antibody on efficient cyhalothrin is 10 micrograms per liter. The antibody has no obvious cross reaction on other pyrethroid pesticides (deltamethrin, cypermethrin, cyfluthrin and fenvalerate).

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a high-efficacy-cyhalothrin hapten, an activated carrier protein, a complete antigen and a polyclonal antibody for immune analysis. Background technique [0002] Pyrethroid insecticides are a class of synthetic insecticides that mimic natural pyrethrins and are insect neurotoxins biosynthesized from pyrethrins. So far, pyrethroids have occupied more than 18% of the global pesticide market. Lambda-cyhalothrin (as shown in formula 1 below) is one of the important components of this type of insecticide, and is widely used in agricultural production. Although its toxicity is widely believed to be low, the results of two recent studies have raised major questions about the molecule's safety in humans. For example, studies have shown that overexposure to cypropathrin may result in myalgia, acute respiratory irritant injury, and shock. On the other hand, since residues of lambda-cy...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07C235/34C07C231/06C07K14/77C07K16/06C07K16/44
CPCC07C235/34C07K14/77C07K16/44C07K16/065C07C2601/02
Inventor 曹立民崔南隋建新林洪韩香凝孙逊
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap