Continuous preparation process of zanthoxylum bungeanum seed protein

Abstract

The invention provides a continuous preparation process of zanthoxylum bungeanum seed protein, which comprises the following steps: pretreating zanthoxylum bungeanum seeds, extracting grease by adopting a subcritical process, and collecting degreased zanthoxylum bungeanum seed meal; carrying out superfine grinding on zanthoxylum bungeanum seed meal, sieving, carrying out ultrasonic hedging countercurrent reaction together with alkali liquor, wherein when the ultrasonic hedging countercurrent reaction is performed, the system is alkalescent; obtaining protein slurry after the ultrasonic hedgingcountercurrent reaction; enabling the protein slurry to pass through an ultrasonic vibrating screen and then a centrifugal machine to screen out insoluble substances twice, so as to obtain protein clear liquid; and adding an acid solution into the protein clear liquid to regulate the system to be weakly acidic, separating by using a centrifuge, and carrying out freeze drying by using a freeze dryer to obtain the zanthoxylum bungeanum seed protein. The continuous preparation process combining subcritical degreasing, ultrasonic oscillation continuous extraction, weak acid settling separation and high-speed freezing separation is adopted, the finished zanthoxylum bungeanum seed protein product with good quality and high yield is obtained, the reaction time is short, and the production efficiency is high.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a continuous preparation process of prickly ash seed protein. Background technique [0002] Zanthoxylum bungeanum is the main by-product in the production of Zanthoxylum bungeanum peel, which contains 18% protein in addition to rich oil. At present, the research on the extraction process of Zanthoxylum bungeanum protein is based on laboratory data or refers to the separation process of animal protein and soybean protein. The common degreasing process of protein raw materials mostly uses solvents such as ethanol and petroleum ether for degreasing. These solvents are not only costly, but also have solvent residues that interfere with subsequent processing processes and traditional high-temperature processes that denature the prepared proteins to varying degrees. In addition, the protein separation process after degreasing is mostly intermittent processes such as alkali-soluble acid an...

AI Technical Summary

Problems solved by technology

The common degreasing process of protein raw materials mostly uses solvents such as ethanol and petroleum ether for degreasing. These solvents are not only costly, but also have solvent residues that interfere with subsequent processing processes and traditional high-temperature processes that cause denaturation of the prepared proteins to varying degrees.

In addition, the protein separation process after degreasing is mostly intermittent processes such as alkali-soluble acid analysis method, enzymatic hydrolysis method, membrane separation method, ion exchange method, ultrafiltration concentration method, etc., which require a long time for reaction and precipitation in the tank