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Probiotics delivery microcapsule based on lentinus edodes soluble dietary fiber and preparation method

A dietary fiber, soluble technology, applied in the direction of bacteria used in food preparation, food forming, function of food ingredients, etc., to achieve the effects of no toxic side effects, good encapsulation efficiency, and improved activity and stability

Pending Publication Date: 2020-07-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lentinus edodes soluble dietary fiber can effectively affect the growth of bacteria such as Bacteroides and Bifidobacteria in the intestinal tract of the host, but so far, there is no report on a probiotic delivery microcapsule based on lentinus edodes soluble dietary fiber and its preparation method

Method used

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  • Probiotics delivery microcapsule based on lentinus edodes soluble dietary fiber and preparation method
  • Probiotics delivery microcapsule based on lentinus edodes soluble dietary fiber and preparation method
  • Probiotics delivery microcapsule based on lentinus edodes soluble dietary fiber and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A preparation method for delivering microcapsules of probiotics based on shiitake mushroom soluble dietary fiber, comprising the steps of:

[0047] (1) After the shiitake mushrooms (Lentinus edodes) are crushed, according to the ratio of solid to liquid 1:20, add ethanol aqueous solution with a volume concentration of 75%, heat and reflux for extraction for 1.5 hours, filter, and repeat the heating and reflux extraction for 3 times, and filter the residue , the solid is dried to obtain the mushroom residue;

[0048] (2) Mix the shiitake mushroom residue with deionized water so that the water content is 30%, place it in an autoclave, and autoclave at 120°C and 0.1MPa for 30 minutes to obtain the autoclaved shiitake mushroom material; Deionized water of 20 times the mass of the mushroom material after pressure treatment, extract at 100°C for 2h, filter, and extract twice, combine the filtrate, adjust the pH to 9.0, add 0.2% alkali equivalent to the mass of the mushroom ma...

Embodiment 2

[0056] A preparation method for delivering microcapsules of probiotics based on shiitake mushroom soluble dietary fiber, comprising the steps of:

[0057] (1) After the shiitake mushrooms (Lentinus edodes) are crushed, according to the ratio of solid to liquid 1:15, add ethanol aqueous solution with a volume concentration of 80%, heat and reflux for extraction for 2 hours, filter, and repeat the heating and reflux extraction for 2 times for the residue, filter, The solid is dried to obtain the mushroom residue;

[0058] (2) Mix the shiitake mushroom residue with deionized water so that the water content is 20%, place it in an autoclave, and autoclave at 115°C and 0.1MPa for 45min to obtain the autoclaved shiitake mushroom material; The deionized water of 15 mass times of the mushroom material after the pressure treatment is extracted at 80 ° C for 2 hours, filtered, and extracted 3 times, the filtrate is combined, the pH is adjusted to be 10.5, and 0.1% of the alkali of the qu...

Embodiment 3

[0063] A preparation method for delivering microcapsules of probiotics based on shiitake mushroom soluble dietary fiber, comprising the steps of:

[0064] (1) After the shiitake mushrooms (Lentinus edodes) are crushed, according to the ratio of solid to liquid ratio of 1:25, add ethanol aqueous solution with a volume concentration of 50%, heat and reflux for extraction for 1 hour, filter, and repeat the heating and reflux extraction for 3 times, and filter the residue. The solid is dried to obtain the mushroom residue;

[0065] (2) Mix the shiitake mushroom residue with deionized water so that the water content is 25%, place it in an autoclave, and autoclave at 118°C and 0.2MPa for 15 minutes to obtain the autoclaved shiitake mushroom material; The deionized water of 25 mass times of the mushroom material after the pressure treatment was extracted at 100°C for 0.5h, filtered, extracted twice, the filtrate was combined, the pH was adjusted to 8.5, and 0.5% of the mass of the mu...

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Abstract

The invention discloses a probiotics delivery microcapsule based on lentinus edodes soluble dietary fiber and a preparation method. The method comprises the following steps: (1) after crushing lentinus edodes, carrying out extraction with an ethanol aqueous solution to obtain lentinus edodes; (2) adding water, performing autoclaved treatment, conducting water extraction, adding alkaline protease for reaction, adding alpha-amylase for reaction, and performing enzyme deactivation to obtain an enzymolysis liquid; (3) carrying out concentration, adding ethanol, and carrying out standing, centrifugation and drying to obtain lentinus edodes soluble dietary fiber; and (4) uniformly mixing a probiotics aqueous suspension and a sterile sodium alginate aqueous solution, dropwise adding the mixture into a calcium chloride aqueous solution with stirring, after stirring and hardening, performing water washing to obtain a calcium alginate microcapsule, immersing the calcium alginate microcapsule ina lentinus edodes soluble dietary fiber aqueous solution, and carrying out stirring, washing and drying to obtain the probiotics delivery microcapsule based on lentinus edodes soluble dietary fiber. The microcapsule has a high embedding rate, improves the activity and stability of probiotics, has a synergistic promotion function on human intestine health, and is simple to operate, economical and easy.

Description

technical field [0001] The invention relates to the field of probiotic microencapsulation delivery, in particular to a probiotic delivery microcapsule based on mushroom soluble dietary fiber and a preparation method thereof. Background technique [0002] Today, as people's awareness of health care continues to increase, people are more inclined to choose fermented foods and supplements containing probiotics in their daily diet to prevent physical diseases and maintain health. Probiotics are delivered orally and integrated into the gut system, where strains capable of permanent colonization will interact closely with the host mucosal or epithelial cells, the immune system, and the autogenous microbiota. They play an important role in maintaining human health, promoting healthy regulation of intestinal flora, and participating in the treatment of various diseases, such as diabetes, obesity, non-alcoholic fatty liver disease, hyperlipidemia, etc. Lactobacillus plantarum, as a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L33/21A23L31/00A23P10/30A23L5/00A23C9/13A23C9/123
CPCA23L33/135A23L33/21A23L31/00A23P10/30A23L5/00A23C9/1307A23C9/1234A23C9/1238A23V2002/00A23V2400/175A23V2400/169A23V2400/519A23V2400/533A23V2200/3204A23V2200/3202
Inventor 陈海霞薛梓涵
Owner TIANJIN UNIV
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