Application of radix achyranthis bidentatae polysaccharide to preparation of medicines for preventing non-small cell lung cancer metastasis
A non-small cell lung cancer and Achyranthes knuckle polysaccharide technology, which is applied in the field of medicine, can solve the problems of inhibiting the metastasis of non-small cell lung cancer, achieve the effect of preventing tumor metastasis, improving treatment effect, and inhibiting migration
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Embodiment 1
[0026] Example 1 The inhibitory effect of Achyranthes knuckle polysaccharides on the adhesion of non-small cell lung cancer cells to the extracellular matrix.
[0027]The A549 and PC9 cells in the logarithmic growth phase were digested with trypsin and resuspended in the culture medium containing different Achyranthes bidentata polysaccharides (0, 10, 50, 100 μg / mL), and seeded at 5000 cells per well in the pre- 96-well plates coated with gelatin. Incubate in a 37°C incubator for 1 hour, remove non-adherent cells, add thiazolyl blue (MTT) to a 96-well plate at a final concentration of 1 mg / L, and incubate for 4 hours. Cell supernatant was removed, 100 μL DMSO was added, and the absorbance value at 490 nm was detected on a microplate reader, and the adhesion rate of non-small cell lung cancer cells to the extracellular matrix was calculated, and the results in Table 1 were obtained.
[0028] As shown in Table 1, in A549 and PC9 cells, the adhesion rate of cells in the control ...
Embodiment 2
[0032] Example 2 The inhibitory effect of Achyranthes knuckle polysaccharides on the adhesion of non-small cell lung cancer cells to vascular endothelial cells.
[0033] Human umbilical vein endothelial cells (HUVECs) were obtained from the umbilical cord of newborns delivered by healthy puerperas, and were isolated, identified and subcultured according to the method established in the laboratory of our inventor's unit. The HUVECs cells used in the present invention are all within 8 passages. The HUVECs cells in the logarithmic growth phase were inoculated in a 24-well plate covered with gelatin, and when the cells grew to 90%, the stimulating factor TNFα (10ng / ml) and different concentrations of Achyranthes bidentata polysaccharides (0, 10, 50, 100 μg / ml) were added. mL), after acting for 4 hours, rhodamine-123-stained A549 and PC9 cells were added to 24-well plates (approximately 15×10 4 cells) and incubated in a 37°C incubator for 1 h. Remove unattached tumor cells, and a...
Embodiment 3
[0038] Example 3 Cytotoxic effect of Achyranthes knuckle polysaccharide on non-small cell lung cancer cells and human umbilical vein endothelial cells (HUVECs).
[0039] The A549, PC9 and HUVECs cells in the logarithmic growth phase were cultured in a 96-well culture plate after trypsinization treatment, and the number of cells per well was about 5000. After culturing overnight, different concentrations of polysaccharides of Achyranthes bidentata (0, 1, 10, 50, 100 μg / mL) were added to continue culturing for 24 hours, and thiazolium blue (MTT) was added with a final concentration of 1 mg / L for 4 hours. Remove the cell supernatant, add 100 μL DMSO, and detect the absorbance at 490 nm with a microplate reader, and the results in Table 3 are obtained.
[0040] As shown in Table 3, even at the concentration of 100 μg / mL Achyranthes bidentata polysaccharide, it has almost no effect on the viability of A549, PC9 and HUVECs, verifying that Achyranthes bidentata polysaccharide has no ...
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